| Budget Amount *help |
¥51,000,000 (Direct Cost: ¥51,000,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1985: ¥50,000,000 (Direct Cost: ¥50,000,000)
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| Research Abstract |
A time-resolved microspectrophotometer has been constructed by using Nd:YAG and dye pulse lasers as light source, spectrograph for spectrometry, and synchroscan streak camera for accumulation and processing of fluorescence decay. Focussing laser beam onto a small spot area of the specimen was done by using a fluorescence microscope. This instrument permits us to measure fluorescence with resolutions in three different dimensions, time, space, and wavelength, the ultimate goal being 20 ps, submicrometers, and several nanometers, respectively. With this instrument, we have already made the time-resolved fluorescence measurements on three basic systems; 1) proteins, bovine serum albumin labeled with IAEDANS in water-glycerol, 2) liposomes containing 12AS or 8AN, and 3) erythrocyte ghostcells with DTAF-labeled band 3 proteins. The laser pulses were focused on a spot of 1.6-2 um and the fluorescence signals were accumulated for 7-50 s with the synchroscan streak camera. Fluorescence decay curves of good S/N ratios were obtained and the characteristic decay times were estimated.
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