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Photosynthetic Genes and Their Expression

Research Project

Project/Area Number 61304004
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionOkazaki National Research Institutes National Institute for Basic Biology

Principal Investigator

MURATA Norio  Okazaki Natl. Res. Inst., Natl. Inst. Basic Biol., Prof., 基礎生物学研究所, 教授 (90011569)

Co-Investigator(Kenkyū-buntansha) OHYAMA Kanji  Kyoto Univ., Fac. Agr., Associate Prof., 農学部, 助教授 (40135546)
IZUI Katsura  Kyoto Univ., Fac. Sci., Associate Prof., 理学部, 助教授 (20025414)
KOBAYASHI Hirokazu  Nagoya Univ., Radioisotope Center, Research Associate, アイントープ統合センター, 助手 (80170348)
TAKABE Teruhiro  Meijo Univ., Fac. Sci. & Tech., Associate Prof., 理工学部, 助教授 (80097766)
WATANABE Akira  Nagoya Univ., Fac. Agr., Prof., 農学部, 教授 (70023471)
小保方 潤一  北海道大学, 遺伝子実験施設, 助手 (50185667)
佐々木 幸子  京都大学, 農学部, 助手 (00026519)
高宮 建一郎  九州大学, 理学部, 助教授 (80037259)
和田 敬四郎  大阪大学, 理学部, 助教授 (70028174)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1986: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsPhotosynthetic gene / photosystem I complex / photosystem II complex / cytochrome b / c complex / Rubisco / 光化学系I複合体 / PEPカルボギシラーゼ / チトクロームbーc複合体 / RuBisCO / 光化学系【II】複合体 / チトクロームb-C複合体 / 葉緑体ゲノムDNA
Research Abstract

A low-molecular-mass subunit of photosystem II complex was purified and its partial amino acid seguence was determined. It was found that its gene is encoded by the choloroplast genome, and the gene was termed as psnK. Precursors of the extrinsic photosystem II proteins, 33-kDa and 23-kDa proteins were synthesized from their cDNAs by in vitro transcription and translation system, and their first precessing sited were determined with the stromal processing enzyme activity. The gene for the extrinsic 33-kDa protein of a cyanobacterium was expressed in E. coli, and the processing site of this protein after the expression in E. coli was determined.
2. Action spectra for the light-induced transcription of the Rubisco small subunit and large subunit genes (rbcS and rbcL) were measured. the result suggests that the transcription of rbcS is induced by phytochrome Pfr and that the transcription of rbcL is induced also by phytochrome Pfr, but in addition, by a blue light-absorbing pigment. It was disclosed that methylation of plastid DNA regulates the transcription of rbcL in the non-photosynthetic cells of higher plants. A new Rubisco gene was cloned from a photosynthetic bacterium Chromatium vinosum, and it was found that this gene, but not the previously cloned gene, is expressed in the bacterium.
3. The cDNA and genomic DNA for maize PEP carboxylase were cloned and their nucleotide sequences were determined. The cDNA for aspartate aminoacyltransferase from Panicum maximam was cloned and its nucleotide sequence was determined.
4. The cDNA for a 15.5 kDa subunit of tobacco photosystem I complex was cloned and its nucleotide sequence was determined.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (83 results)

All Other

All Publications (83 results)

  • [Publications] Murata,N.: FEBS Lett.235. 283-288 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyao,M.: Biochim.Biophys.Acta. 936. 465-474 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamazaki,M.: Plant Cell physiol.27. 443-452 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shinohara,K.: Arch.Biochem.Biophys.260. 452-460 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Minami,E.: Plant Cell Physiol.29. 1303-1309 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Oh-oka,H.: FEBS Lett.218. 52-54 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Wada,K.: Biochem.J.252. 571-575 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanaka,S.: Photosyn.Res.17. 255-266 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanaka,S.: Biochemistry. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takabe,T.: Plant Cell Physiol.30. 85-90 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takabe,T.: J.Biochem.105. 98-102 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Iba,K.: J.Biochem.102. 1511-1518 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takamiya,K.: J.Biochem.103. 755-758 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takabe,T.: Plant physiol.88. 1120-1124 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasaki,Y.: FEBS Lett.239. 199-202 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshda,K.: Photochem.Photobiol.印刷中. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Macherel,D.: FEBS Lett.201. 315-320 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kobayashi,H.: Aqric.Biol.Chem.52. 277-279 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ngernprasirtsiri,J.: Proc.Natl.Acad.Sci.USA. 85. 4750-4754 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ngernprasirtsiri,J.: Plant physiol.88. 16-20 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Izui,K.: Nucl. Acids Res.14. 1615-1628 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yanagisawa,S.: FEBS Lett.229. 107-110 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Numazawa,T.: Arch.Biochem.Biophys.(1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohyama,K.: Thends Biochem. Sci.13. 19-22 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanaka,M.: Mol.Gen.Genet.209. 427-431 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Murata, N.: "Identification of a new gene in the chloroplast genome encoding a low-molecular-mass polypeptide of photosystem II complex." FEBS Lett.235. 283-288 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyao, M.: "Partial degradation of the extrinsic 23-kDa protein of the oxygen-evolving photosystem II complex of spinach." Biochim. Biophys. Acta. 936. 465-474 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamazaki, M.: "Nitrogen regulated accumulation of mRNA and protein for photosynthetic carbon assimilating enzymes in maize." Plant Cell Physiol.27. 443-452 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shinohara, K.: "Synthesis and assembly of H^+-ATPase complex by isolated "rough" thylakoids." Arch. Biochem. Biophys.260. 452-460 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Minami, E.: "Localization and properties of transcripts of psbA and rbcL genes in the stroma of spinach chloroplast." Plant Cell Physiol.29. 1303-1309 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Oh-oka, H.: "The 8 kDa polypeptide in photosystem I is a probable candidate of an iron-sulfur center protein coded by the chloroplast gene frxA." FEBS Lett.218. 52-54 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Wada, K.: "Properties and structure of the soluble ferredoxin from Synechococcus 6301 (Anacystis nidulans). Relationship to gene sequences." Biochem. J.252. 571-575 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanaka, S.: "The status of cysteine residues in the extrinsic 33 kDa protein of spinach photosystem II complexes" Photosyn. Res.17. 255-266 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanaka, S.: "Extrinsic 33 kDa protein of spinach oxygen-evolving complexes: Kinetic studies of folding and disulfide reduction." Biochemistry. in press. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takabe, T.: "Electron transfer between plastocyanin and P700 in various types of photosystem O complex." Plant Cell Physiol.30. 85-90 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takabe, T.: "Kinetic studies on a cross-linked complex between plastocyanin and cytochrome f." J. Biochem.105. 98-102 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Iba, K.: "Structural gene of cytochrome b-562 from the cytochrome b-c_1 complex of Rhodobacter sphaeroides." J. Biochem.102. 1511-1518 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takamiya, K.: "Enzymatic properties of ubiquinol:cytochrome c_2 oxidoreductase in situ in Rhodopseudomonas palustris membranes." J. Biochem.103. 755-758 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takabe, T.: "CO_2 fixation rate and RuBisCO content increase in the halotolerant cyanobacterium, Aphanothece halophytica, grown in high salinities." Plant Physiol.88. 1120-1124 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasaki, Y.: "Action spectra for photogene expression in etiolated pea seedlings." FEBS Lett.239. 199-202 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshida, K.: "Fluence-response relationship for photogene expression in etiolated pea seedlings." Photochem. Photobiol.in press. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Macherel, D.: "Expression of amyloplast DNA in suspension-cultured cells of sycamore (Acer pseudoplatanus L.)." FEBS Lett.201. 315-320 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kobayashi, H.: "A rapid DNA sequencing procedure: Unidirectional deletion of DNA fragments and use of reverse transcriptase in sequencing reaction." Agric. Biol. Chem.52. 277-279 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ngernprasirtsiri, J.: "DNA methylation as a mechanism of transcriptional regulation in nonphotosynthetic plastids in plant cells." Proc. Natl. Acad. Sci. USA. 85. 4750-4754 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ngernprasirtsiri, J.: "NDA methylation occurred around lowly expressed gene of plastid DNA during tomato fruit development." Plant Physiol.88. 16-20 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Izui, K.: "Cloning and sequence analysis of cDNA encoding active phosphoenolpyruvate carboxylase of the C_4-pathway from maize." Nucl. Acids. Res.14. 1615-1628 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yanagisawa, S.: "Further analysis of cDNA clones for maize phosphoenolpyruvate carboxylase involved in C_4 photosynthesis: Nucleotide sequence of entire open reading frame and evidence for polyadenylation of mRNA at multiple sites in vivo." FEBS Lett.229. 107-110 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Numazawa, T.: "Aspartate aminotransferase from Panicum maximum Jacq. var. trichoglume Eyles, a C_4 plant: Purification, molecular properties and preparation of antibody." Arch. Biochem. Biophys.in press. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohyama, K.: "Newly identified groups of genes in chloroplast." Trends Biochem. Sci.13. 19-22 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanaka, M.: "Rapid splicing and stepwise processing of a transcript from the psbB operon in tobacco chloroplasts: Determination of the intron sites in petB and petD." Mol. Gen. Genet.209. 427-431 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Murata,N.: FEBS Lett.235. 283-288 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Miyao,M.: Biochim.Biophys.Acta. 936. 465-474 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shinohara,K.: Arch.Biochem.Biophys.260. 452-460 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Minami,E.: Plant Cell Physiol.29. 1303-1309 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tanaka,S.: Photosyn.Res.17. 255-266 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tanaka,S.: Biochemistry. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takabe,T.: Plant Cell Physiol.30. 85-90 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takabe,T.: J.Biochem.105. 98-102 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takamiya,K.: J.Biochem.103. 755-758 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takabe,T.: Plant Physiol.88. 1120-1124 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sasaki,Y.: FEBS Lett.239. 199-202 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshida,K.: Photobiol.Photobiochem.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kobayashi,H.: Agric.Biol.Chem. 52. 277-279 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ngernprasirtsiri,J.: Proc.Natl.Acad.Sci.USA. 85. 4750-4754 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ngernprasirtsiri,J.: Plant Physiol.88. 16-20 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ngernprasirtsiri,J.: J.Biol.Chem.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Viale,A.M.: J.Bacteriol.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yanagisawa,S.: FEBS Lett.229. 107-110 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Numazawa,T.: Arch.Biochem.Biophys.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohyama,K.: Trends Biochem.Sci.13. 19-22 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tanaka,M.: Mol.Gen.Genet.209. 427-431 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shinohara,K.;Minami,E.;Watanabe,A.: Arch.Biochem.Biophys.260. 452-460 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Koh Iba;Ken-ichirou Morohashi;Toshiyuki Miyata;Ken-ichiro Takamiya: Journal of Biochemistry. 102. 1511-1518 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Y.Sasaki;Y.Nakamura;R.Matsuno: Plant Mol.Biol.8. 375-382 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] H.Kobayashi;L.Bogorad;C.D.Miles: Plant Physiol.85. 757-767 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kanji Ohyama et al: Trends in Biochemical Sciences. 13. 19-22 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Obokata,J.: Plant Physiology. 84. 535-540 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] E.Minami;K.Shinozaki;T.Kuwabara;A.Watanabe: Archives of Biochemistry and Biophysics. 244. 517-527 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Ohyama他: Nature. 322. 572-574 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Shinozaki他: Plant Molecular Biology. 4. 110-147 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Kuwabara;T.Murata;M.Miyao;N.Murata: Biochimica et Biophysica Acta. 850. 146-155 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Oh-oka;S.Tanaka;K.Wada;T.Kuwabara;N.Murata: FEBS Letters. 197. 63-66 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Takabe;T.Takabe;T.Akazawa: Plant Physiology. 81. 60-66 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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