| Project/Area Number |
61304019
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
蚕糸学
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| Research Institution | Shinshu University |
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Principal Investigator |
YANAGISAWA YUKIO Shinshu University, 繊維学部, 助教授 (70021160)
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| Co-Investigator(Kenkyū-buntansha) |
OHNISHI TOSHIO Kyoto University of Industrial Arts and Textile Fibers, 繊維学部, 助教授 (20027874)
大山 勝夫 農水省, 農業生物資源研究所・細胞育種部, 部長
OSHIGANE KENGO Shinshu University, 繊維学部, 助教授 (40021159)
KUNO KATSUJI Tokyo University of Agriculture and Technology, 農学部, 助教授 (70092484)
HOMMA SHIN Tokyo University of Agriculture and Technology, 農学部, 教授 (70014941)
OHOYAMA KATUO National Institute of Agrobiological Resources
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 1988: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1987: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1986: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | Elongation of mulberry branch, Inorganic matter / Accumulation / Embryogenesis / Preservation / tissue culture / Protoplast fusion of mulberry and paper-mulberry / サイトカイニン / 集積 / 日伸長量 / コルヒチン培養芽 / 桑と椿の電気融合 / ゼアチン含量 / 気温 / 桑の生長 / 成分の移行率 / 胚様体 / 未熟胚培養 / 不定芽形成 / 融合率 / 生長パターン / 品種間差異 / 無機元素 / 葯培養 / 腋芽培養 / 液体培養 / 桑プロトプラスト / 内生生長物質 |
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| Research Abstract |
The results obtained from studies on the title of project are summarized as follows. 1) Homma made a comperison of mulberry growth in three latitudes: Murayama city, Yamagata; Sekishiro cho^^-, Ibaraki; and Togane city, Chiba. Elongation of mulberry plants in Chiba tend to larger, Ibaraki next and that in Yamagata the least. 2) The mulberry varieties of different age of tree were used to study on the rise and fall of inorganic matters in leaves. Accumulation of N and K in leaves became more in elder plant. The most rich leaves in Fe were observed in the variety Riso (KUNO). 3) Anthers culture that used diploid and hexaploid plants, formed its plantlet through the process of embryogensis in the hexaploid of the one plant. Triploid plant bud culture that was treated with colchicine in vitro, found to be capable in part producing the hexaploid (2n=84) and mixoploid plants, respectively (OSHIGANE). 4) Development of the preservation technique for genetic resource mulberry was tried use of tissue culture. The result showed that it was possible propagation of mulberry, which was culture on MS medium BA added. Preservation by shoot tip culture can be preserved over 4th months on MS medium (OHOYAMA). 5) By improving the conventional enzyme solutions for the isolation of protoplasts, the increase in the yield of protoplasts was studied. And the fusion of protopasts. From the "mulberry x mulberry" and "mulberry x paper-mulberry" in polyethylene glycol and electrin method were studied (OHNISHI). 6) Yanagisawa analyzed the seasonal variation of cytokinin content in mulberry of three varieties : Kenmochi, Ichinose, and Rohachi. Some difference of the Z, ZR, and 2iP contents among the three varieties seem to be those ecotype. Z content in winter bud showed close correlation to dormancy of that.
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