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Studies on immunology and molecular biology of host-parasite interactions

Research Project

Project/Area Number 61304037
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionChiba University

Principal Investigator

KOJIMA Somei  Chiba University School of Medicine, 医学部, 教授 (00009622)

Co-Investigator(Kenkyū-buntansha) WATANABE Naohiro  Jikei University School of Medicine, 助教授 (00057019)
SENDO Fujiro  Yamagata University School of Medicine, 医学部, 教授 (80091833)
YOSHIMURA Kentaro  Akita University School of Medicine, 医学部, 教授 (90053058)
SUGANE Kazuo  Shinshu University School of Medicine, 医学部, 教授 (50112488)
TANABE Kazuyuki  Osaka City University School of Medicine, 医学部, 助手 (40047410)
新家 荘平  兵庫医科大学, 教授 (10029770)
新村 宗敏  千葉大学, 医学部, 助教授 (60059095)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥19,000,000 (Direct Cost: ¥19,000,000)
Fiscal Year 1988: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1987: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1986: ¥9,000,000 (Direct Cost: ¥9,000,000)
KeywordsHost-parasite relationship / antigenic variation / parasite antigen genes / monoclonal IgE / protective immunity / 免疫変調 / 宿主 / 寄生虫相互作用
Research Abstract

To better understand host-parasite interactions, immunological studies were carried out on cellular and molecular levels. 1) Adaptation of protozoan parasites in hosts and their antigenic variation: Plasmodium falciparum was found to poliferate in human eryhrocytes without being affected by levels of Ma^+ or K^+, although the parasite died easily by treatment with Ca^<2+> modulators. A 25kDa molecule was found to be specific to all stages of trypanosoma cruzi, while antigcnic variation of T.gambiense occurred in vitro in the abscence of monoclonal antibodies about 100 times more frequently than in vivo. 2) Analysis and gene cloning of parasite antigens: The complete nucleotide sequence of a surface antigen (p190) of P.falciparum merozoites was determined and comparisons of the gene from various strains revealed that the p190 gene consisted of several blocks of conserved, semi-conserved and variable sequneces, the last of which were not widely polymorphic but fell into two types. Using … More mRNA of Trichinella spiralis larvae, 97% of cDNA coding an antigenic molecule of 48kDa was obtained. A mouse monoclonal IgE antibody was prepared against a 97kDa surface molecule of Schistosoma iaponicum. Results of chemical analysis suggested that the molecule was a glycoprotein containing a complex of sugar chains bound to asparagine. 3) Disturbance of host immune mechanisms by parasitic infections: Increase of OKT4- and OKla-1- positive cells was observed in patients with strongyloidiasis in association with decrease of OKT8-positive cells. Proliferative responses of lymphocytes to mitogens significantly reduced in these patients if compared to the responses in parasite-free controls. Schistosome infections lead to reduction in IL-2 production and responsiveness to IL-1 and IL-2, in addition to polyclonal b cell activation. It was also suggested that eosinophils had receptors to chemotactic factors derived from young adult worms or first stage larvae of Angiostrongylus cantonensis. Furthermore, IgE-bearing B cells increased in number after helminth infections in association with increase of quanitity of the surface IgE. Less

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Tanabe,K.et al.: J.Mol.Biol.195. 273-287 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kojima,S.et al.: J.Immunology. 139. 2044-2049 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita,T.et al.: Immunology. 62. 215-221 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Watanabe,N.et al.: Proc.Natl.Acad.Sci.USA. 85. 4460-4462 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tachibana,H.et al.: parasitol.Res.74. 409-414 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tnabe,K.,et al.: "Allelic dimorphism of a surface antigen gene of the malaria parasite Plasmodium falciparum." J. Mol. Biol.195. 273-287 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kojima,S.,et al.: "Production and properties of a mouse monoclonal IgE antibody to Schistosoma japonnicum." J. Immunology. 139. 2044-2049 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamashita, T.,et al.: "Studies on the immunological disturbance in murine Schistosomiasis japonica from the viewpoint of the interleukin cascade reaction." Immunology. 62. 215-221 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Watanabe,N.,et al.: "Protective immunity and eosinophilia in IgE-deficient SJA/9 mice infected with Nippostrongylus brasiliensis and Trichinella spiralis." Proc. Natl. Acad. Sci. USA. 85. 4460-4462 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tachibana, H.,et al.: "Serodiagnosis of Chagas' disease using monoclonal antibody against Trypanosoma cruzi -specific Mr 25000 antigen." Parasitol. Res.74. 409-414 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tanabe,K.,;Mackay,M.,;Goman,M.;Scaife,J.G.: J.Mol.Biol.195. 273-287 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kojima,S.,;Niimura,M.;Kanazawa,T.: Journal of Immunology. 139. 2044-2049 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamashita,T.,;Watanabe,T.;Sendo,F.: Immunology. 62. 215-221 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Watanabe,N.,;Katakura,K.,;Kobayashi,A.,;Okumura,K.;Ovary,Z.: Proc.Natl.Acad.Sci.USA. 85. 4460-4462 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tachibana,H.,;Nagakura,K.;Kaneda,Y.: Parasitol. Res.74. 409-414 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tanabe,K.;Mackay,M.;Goman,M.;Scaife,J.G.: Journal of Molecular Biology. 195. 273-287 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kaneda,Y.;Tachibana,H.;Goutsu,T.: Journal of Parasitology. 73. 658-661 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Watanabe,N.;Kobayashi,A.;Miyajima,H.;Hirano,T.;Ovary,Z.: Journal of Immunological Methods. 96. 41-45 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Azuma,M.;Hirano,T.;Miyajima,H.;Watanabe,N.;Yagita,H.;Enomoto,S.;Furusawa,S.;Ovary,Z. et al.: Journal of Immunology. 139. 2538-2544 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sugaya,H.;Yoshimura,K.: Parasite immunology. 10. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kojima,S.;Niimura,M.;Kanazawa,T.: Journal of Immunology. 139. 2044-2049 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Izumo,A.;Tanabe,K.;Takada,S.: Trans.Roy.Soc.Trop.Med.Hyg.80. 1007-1008 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Izumo,A.;Tanabe,K.;Kato,M.: Trons.Roy.Soc.Trop.Med.Hyg.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Sugane,K;Matsuura,T;Maekawa,H.: J.Helminth.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Ishida,K.;Yoshimura,K.: Z.Parasitenkd.72. 661-671 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Makino,M.;Fujiwara,M.;Watanabe,H.: J.Clin.Lab.Immunol.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kojima,S.;Niimura,M.;Kanazawa,T.: Manuscript in preparation.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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