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ASSESS OF DEVELOPMENTAL HAZARDS DUE TO ENVIROMENTAL FACTORS AT THE STAGES FROM PREFERTILIZATION TO IMPLANTATION

Research Project

Project/Area Number 61304051
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionNagoya City University

Principal Investigator

YAGAMI Yoshiaki  Nagoya City University, Professor, 医学部, 教授 (20079974)

Co-Investigator(Kenkyū-buntansha) SUZUMORI Kaoru  Nagoya City University, Associate Professor, 医学部, 助教授 (80117829)
HIROI Masahiko  Yamagata University, Professor, 医学部, 教授 (60018364)
SHIMIZU Tetsuya  Asahikawa Medical College, Professor, 医学部, 教授 (20000979)
MIZUNO Masahiko  Tokyo University, Professor, 医学部, 教授 (10010175)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥16,000,000 (Direct Cost: ¥16,000,000)
Fiscal Year 1987: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1986: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsEpidermal growth factor(EGF) / Oocyte maturation / Follicular fluid / Gamete intra-fallopian trasfer(GIFT) / Oocyte / Sister Chromatid Exchange(SCE) / in vitro culture / Cryopersevation Microelectrode / Intracellular pH / X-irradiation / CDDP / Chromosome / Early embryo / 染色体異常 / 卵割遅延 / 受精周辺期 / 生殖細胞 / 受精卵の凍結保存法 / 卵成熟抑制因子 / 亜鉛
Research Abstract

1)Epidermal growth factor-like substance was present in human follicular fluid at a mean concentration about 1 ng/ml. The level of EGF-like substance was correlated with the degree of oocyte maturation and progesterone concentration in the follicle. Involvement of EGF in oocyte maturation in vitro was investigated in rats. EGF increased the accumulation of progesterone in preovulatory follicle and oocyte maturation was stimulated by EGF in dose-dependent manner. Thus, it is suggested that EGF may be concerned with oocyte maturation.
2)The methods of cryopreservation and dilution of the unfertilized and fertilized eggs of ICR mouse have been established. The intracellular pH changes in hamster embryos at various stages of development was examined using a microelectrode technique. Slight increase of intracelllular pH in embryo occurred at 2 cell stage and each successive stage.
3)The effects of culture conditions on oocytes and early stage embryos were examined by sister chromatid exchange(SCE) which was a sensitive indicator for cartinogenicity and mutagenecity. The supplement of serum or EDTA decreased SCE. The supplement of sex steroids, cryopreservation of embryos, or silicon container did not have a effect on SCE. The supplement of zinc, which is a co-factor of enzymes, increased SCE. As the initial stage of embryo cultured in vitro advanced, SCE decreased.
4)The effects of X-irradiation and the anti-cancer drug(CDDP) on fertilization and early embryogenesis was investigated with the use of Chinese Hamster oocytes.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] 矢野哲 他: 第39回日本産科婦人科学会. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 矢野哲 他: 第60回日本内分泌学会. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 堤治 他: 第40回日本産科婦人科学会. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 清水哲也.高田久士.石川睦男: 産婦人科の実際. 36. 607-613 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 高田 久士: 日本産科婦人科学会雑誌. 40. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤英和,佐藤文彦,斉藤隆和,楊美貴,広中正彦: 産婦人科の世界. 39. 297-301 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 佐藤文彦.斉藤英和.斉藤隆和.楊美貴.広井正彦: 産婦人科の世界. 39. 429-434 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤英和,佐藤文彦,平山寿雄,斉藤隆和,荒木慶彦,楊美貴,広井正彦: 哺乳動物卵子研究会誌. 4. 39-40 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤 英和: 第4回日本受精着床学会. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 佐藤文彦.斉藤英和.広井正彦: 第31回日本不妊学会. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤英和,佐藤文彦,楊美貴,斉藤隆和,川越慎之助,広井正彦: 第31回日本不妊学会. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Saito; F.Sato; M.Yoh; T.Saito; M.Hiroi: 42nd Annual Meeting of the Americal Fertility Society Sep. 27-Oct. 2 1986. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 佐藤文彦,斉藤英和,平山寿雄,楊美貴,広井正彦: 第32回日本不妊学会. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 勅使河原啓市 今泉克英 岡田節男 鈴森薫 八神喜昭: 第4回日本受精着床学会. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] K.Imaizumi: Asia Oceania J. of Obstet. & Gynecol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岡田節男,鈴森薫,八神喜昭,勅使河原啓市,今泉克英: 第40回日本産科婦人科学会. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 佐藤文彦,斉藤英和,広井正彦: 第39回日本産科婦人科学会. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤英和,佐藤文彦,広井正彦: 第39回日本産科婦人科学会. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Saito; F.Sato; Y.Araki; T.Saito; M.Yoh; M.Hiroi: Fifth World Congress on in vitro fertilization and embryonic Transfer. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Saito; F.Sato; T.Hirayama Y,Araki; T.Saito; M.Yoh M.Hiroi: 43nd Annual Meeting of the American Fertility Society. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] F. Sato: Kyoto International Symposium on Human in vitro Fertilization. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斉藤英和.佐藤文彦.平山寿雄.斉藤隆和.楊美貴.広井正彦: 第5回日本受精着床学会. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yano T, et al.: "Effects of epidermal growth factor on oocyte maturation in vitro." Proceedings of Japan Society of Obstetrics and Gynecology 39th Annual Meeting. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yano, T. et al.: "Involvement of C-kinase in oocyte maturation" Proceedings of Japan Society of Endocrinology 60th Annual Meeting. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tsutsumi, O. et al.: "Epidermal growth factor-like substance in human follicular fluid and oocyte maturation" Proceedings of Japan Society of Obstetrics and Gynecology 40th Annual Meeting. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shimizu, T., Takada, H., and Ishikawa, M.: "Cryopreservation of unfertilized egg" Obstetrical and Gynecological Practice. 36. 607-613 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takada, H.: "Effects of Cryoprotectants and Sucrose Dilution on the Survival of Unfetilized and Fertilized Mouse Eggs after Freezing and Thawing." Acta Obstetrica et Gynecologica Japonica.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Saito, H., Sato, F., Saito, T., Yoh, M., and Hiroi, M.: "Sister Chromatid Exchange of Egg" World Obstet. Gynecol.39. 297-301 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Sato, F., Saito, H., Saito, T., Yoh, M., and Hiroi, M.: "Cryopreservation of Fertilized Oocyte" World Obstet. Gynecol.39. 429-434 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Saito, H. Sato, F., Hirayama, T., Saito, T., Arai, Y., Yoh, M., and Hiroi, M.: "Effects of EDTA on Embryo Growth in vitro" J. Mammarian Ova Research. 4. 39-40 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Imaizumi, K.: "Effects of X-irradiation during Peri-nidation Stage of Gestation -Chromosome Analysis, Cell Viability and Embryo Survival in the Chinese Hamster-" Asia-Oceania J. Obstet. & Gynecol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 矢野哲,北川浩明,堤治,佐藤和雅,水野正彦: (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 矢野哲,提治,綾部琢哉,佐藤和雅,水野正彦: (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 矢野哲,提治,綾部琢哉,佐藤和雅,水野正彦: 哺乳動物卵子研究会誌. 3. 103-113 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 清水哲也,高田久士,石川睦男: 産婦人科の実際. 36. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 高田久士,浅川竹仁,石川睦男,清水哲也: 日本産科婦人科学会誌(講演抄録). 39. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Hidekazu Saito: Fertility & Sterility.

    • Related Report
      1986 Annual Research Report
  • [Publications] 勅使河原啓市,今泉克英,岡田節夫,鈴森薫,八神喜昭: (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Katsuhide Imaizumi: Asia- Oceania J. Obstet & Gynecol.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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