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Structure and Function of Photosensory Pigments in Unicellular Organs.

Research Project

Project/Area Number 61304069
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionSapporo Medical College

Principal Investigator

TSUDA Motoyuki  Sapporo Medical College, 医学部, 助教授 (60045458)

Co-Investigator(Kenkyū-buntansha) WATANABE Masakatsu  National Institute for Basic Biology, 大型スペクトル室, 助手 (40124226)
KOYAMA Yasushi  Kansei Gakuin University, 理学部, 教授 (90079666)
SHICHIDA Yoshinori  Kyoto University, 理学部, 助手 (60127090)
KOUYAMA Tsutomu  The Institute of Physical and Chemical Research, 生物物理研究室, 研究員 (30170210)
KOBAYASHI Takayoshi  University of Tokyo, 理学部, 助教授 (60087509)
高橋 哲郎  北海道大学, 薬学部, 助手 (90133769)
大野 宏毅  自治医科大学, 医学部, 講師 (30049085)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1986: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsSensory Rhodopsin / Phoborhodopsin / Photoreceptor / Halobacteria / 走光性 / ハロバクテリア / 光感覚 / 光化学反応サイクル / 光閃光分解
Research Abstract

Rhodopsin, visual pigment in animal retina, is composed of retinal and seven helices protein. This retinal protein was also found in archibacteria, halobacterium halobium. Bacteriorhodopsin and halorhodopsin were light driven ion pumps. Third pigment, which was found by Tsuda's group and Spudich's group, was characterized as a photoreceptor for phototaxis. In addition to sR, phoborhodopsin (pR) was found as a fourth pigment in holobacteria, which is a receptor for photorequlsion (Kamo, Takahashi). It was found that another unicellular orgam, Clamydomonas contains rhodopsinlike pigment which reacted with antibody against frog rhodopsin (Shinozawa). Photochemical process of sensory rhodopsin was studied by laser flash photolysis and new intermediate sR_L was found in in addition to sR_K and sR_M (Kobayashi, Tsuda). Photocycle of phoborhodopsin was studied by low temperature spectroscopy and P520 was found to form below -50゜C. Conformation of retinal in these pigments was studied by chemical extraction (Tsujimoto) and resonance Raman spectroscopy (Koyama) and shown that photochemical event in these pigments are photoisomerization between all trans retinal and 13゜cis retinal. In order to sutdy interaction between retinal and protein in these pigments, spin labeled retinal (Kusumi) and fluorescence labeled retinal (Kouyama) were used. Phototransduction in phototaxitic unicellular species is not known. It was shown that sensory rhodopsin did not transport proton (Ohno). However, G-protein was found in these organs (Watanabe).

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] 高橋哲郎、津田基之: 蛋白質・核酸・酵素. 34(4). 452-461 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohtani,H.;Kobayashi,T.;and Tsuda,M.: Biophys.J.53(4). 493-496 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tomioka,H.;Takahashi,T.;Kamo,N.;Kobatake,Y.: Biochem.Biophys.Res.Commun.139. 389-395 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shichida,Y.;Imamoto,Y.;Yoshizawa,T.;Takahashi,T.;Tomioka,H.;Kamo,N.;Kobatake,Y.: FEBS Letters. 236. 333-336 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mukai,Y.;Koyama,Y.;Hirata,Y.;Mataga,N.: J.Phys.Chem.92. 4649-4653 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hasunuma,K.;Furukawa,K.;Funadera,K.;Kubota,M.;Watanabe,M.: Photochem.Photobiol.46. 531-535 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuda,M.: "Pressure Effect on Three Retinal Proteins in Halobacterium halobium.In Biophysical Studies of Retinal Proteins," University of Illinois Press,Champaign, 304 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kobayshi,T.;Ohtani,H.;Tsuda,M.: "Photocycles of Sensory Rhodopsin.In Primary Process in Photobiology," Springer-Verlag,Berlin, 243 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohtani, H.; Kobayashi, T.; Tsuda, M.: "Branching Photocycle of Sensory Rhodopsin in Halobacterium halobium." Biophys. J.53(4). 493-496 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tomioka, H.; Takahashi, T.; Kamo, N.; Kobatake, Y.: "Flash Spectrophotometric Identification of a Fourth Rhodopsin-like Pigment in Halobacterium halobium" Biochem. Biophys. Res. Commun.139. 389-395 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shichida, Y.; Imamoto, Y.; Yoshizawa, T.; Takahashi, T.; Tomioka, H.; Kamo, N.; Kobatake, Y.: "Low-Temperature Spectrophotometry of Phoborhodopsin." FEBS Letters. 236. 333-336 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mukai, Y.; Koyama, Y.; Hirata, Y.; Mataga, N.: "Configurational Changes of Retinal in the Triplet State: Picosecond Time-Resolved Absorption Spectroscopy on the 7-cis, 11-cis, and 13-cis Isomers and High-Performance Liquid Chromatography Analysis of Photoisomerization." J. Phys. Chem.92. 4649-4653 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hasunuma, K.; Furukawa, K.; Funadera, K.; Kubota, M.; Watanabe, M.: "Partial Characterization and Light-Induced Regulation of GTP-binding Proteins in Lemna Paucicostata." Photochem. Photobiol.46. 531-535 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuda, M.: Pressure effect of Three Retinal Proteins in Halobacterium halobium In Biophysical Studies of Retinal Proteins.University of Illinois Press, Champign, 38-43 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 高橋哲郎、津田基之: 蛋白質・核酸・酵素. 34(4). 452-461 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohtani,H.;Kobayashi,T.;Tsuda,M.: Biophys.J.53(4). 493-496 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tomioka,H.;Takahashi,T.;Kamo,N.;Kobatake,Y.: Biochem.Biophys.Res.Commun.139. 389-395 (1986)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shichida,Y.;Imamoto,Y.;Yoshizawa,T.;Takahashi,T.;Tomioka,H.;Kamo,N.;Kobatake,Y.: FEBS Letters. 236. 333-336 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Mukai,Y.;Koyama,Y.;Hirata,Y.;Mataga,N.: J.Phys.Chem.92. 4649-4653 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hasunuma,K.;Furukawa,K.;Funadera,K.;Kubota,M.;Watanabe,M.: Photochem.Photobiol.46. 531-535 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tsuba,M.: "Pressure Effect on Three Retinal Proteins in Halobacterium halobium.In Biophysical Studies of Retinal Proteins." University of Illinois Press,Champaign, 304- (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kobayashi,T,;Ohtani,H,;Tsuda,M.: "Photocycles of Sensory Rhodopsin.In Primary Process in Photobiology." Springer-Verlag., 243 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohtani,H.;Kobayashi,T.;Tsuda,M.: Biophys.J.53. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Takahashi,T.;Yorimitsu,S.;Tsujimoto,K.;Kamo,N.;Kobatake,Y.: Biochem.Biophys.Res.Commun.,. Submit.

    • Related Report
      1987 Annual Research Report
  • [Publications] Ariki,M.;Shichida,Y.;Yoshizawa,T.: FEBS Letters. 225. 255-258 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Hirata,H.;Mataga,N.;Mukai,Y.;Koyama,Y.: Chem.Phys.Lett.134. 166-170 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Hasunuma,K.;Furukawa,K.;Funadera,K.;Kubota,M.;Watanabe,M.: Photochem.Photobiol.46. 531-535 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Shinozawa,T.;Teramoto,Y.;Matsusaka,H.;Horiuchi,K.: Biochimie.

    • Related Report
      1987 Annual Research Report
  • [Publications] Tsuda,M.edt.by Ebrey,et al.: "Pressure Effect on Three Retinal Proteins in Halobacterium halibium.In Biophysical Studies of Retinal Proteins" University of Illinois Press,Champaign, 304 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kobayashi,T.;Ohtani,H.;Tsuda,M.edit by Kobayashi,T.: "Photocycles of Sensory Rhodopsin.In Primary Process in Photobiology" Springer-Verlag,Berlin, 241 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ohtani,H.;Kobayashi,T.;Tsuda,M.: Biophys.J.in press. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Ohtani,H.;Kobayashi,T.;Tsuda,M.: Photobiochem.Photobiophys.in press. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tomioka,H.;Takahashi,T.;Kamo,N.;Kobatake,Y.: Biochem.Biophys.Res.Commun.139. 389-395 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tomioka,H.;Takahashi,T.;Kamo,N.;Kobatake,Y.: Biochim.Biophys.Acta.884. 578-584 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Otomo,J.;Ohno,K.;Takeuchi,Y.;Ikegami,A.: Biophys.J.50. 205-211 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tsujimoto,K.;Yorimitsu,S.;Ohashi,M;Takahashi,T.;Tsuda,M.: Chem.Lett.,. in press. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tsuda,M.: "Pressure Effect on Three Retinal Proteins in Halobacterium halobium.In Biophysical Studies of Retinal Proteins.(edit by Nakanishi)" University of Illinois Press,Champaign, (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Govindjee,R.;Dancshazy,Z.;Tsuda,M.: "Photochemistry of Tyrosine-64 Nitrated Bacteriorhodopsin.In Biophysical Studies of Retinal Proteins.(edit by Nakanishi)" University of Illinois Press,Champaign, (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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