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Controls of gene expression in lower eukaryotes

Research Project

Project/Area Number 61440014
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

TSUCHIYA Eiko (1988)  Fac. Engineering, Hiroshima Univ., Research Assistant, 工学部, 助手 (90127671)

福井 作蔵 (1986-1987)  広島大学, 工学部, 教授 (60013299)

Co-Investigator(Kenkyū-buntansha) FUKUI Sakuzo  Fac. Engineering, Hiroshima Univ., Professor Emelitus, 工学部, 名誉教授 (60013299)
KAMIRYO Tatsuyuki  Fac. Integrated Art and Science, Hiroshima Univ., Associate Professor, 総合科学部, 助教授 (50025649)
MIYAJAWA Tokichi  Fac. Engineering, Hiroshima Univ., Professor, 工学部, 教授 (10116676)
TOH-E Akio  Fac. Engineering, Hiroshima Univ., Professor, 工学部, 教授 (90029249)
土屋 英子  広島大学, 工学部, 助手 (90127671)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥26,000,000 (Direct Cost: ¥26,000,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1986: ¥21,000,000 (Direct Cost: ¥21,000,000)
KeywordsSporulation / 2 um DNA / Peroxisome / Transmembrane signaling / Ca^<2+> / calmodulin depandent protein kinase / Cell division cycle / DNA replication / 核膜の物質輸送 / 下等真核生物 / オルガネラ形成 / 核膜ATPase / 性分化 / トランスメンブレンシグナリング機構 / DNAの核内輸送
Research Abstract

In the aim of analyzing the controls of gene expression during the cell proliferation and differentiation of yeasts, we accomplished this project and obtained the following findings.
1. Fukui cloned the glucoamylase gene (SGA) specifically expressed during the sporulation of Saccharomyces cerevisiae and determined its DNA sequence.
2.Toh-e isolated 4 novel 2 mum-DNA-like plasmids (pSB2, pSB3, pSR1 and pSM1) from Zygosaccharomyces yeasts and determined the DNA sequences involved in autonomous replication, stable maintenance and recombination of the plasmids. A host-vector system for Zygosaccharomyces rouxii was developed by using pSR1.
3. Kamiryo cloned 9 genes encoding peroxisomal proteins (POXs and OLE) and found that POX2 and POX4, and POX5 and OLE3 formed clusters in Candida tropicalis genom.
4. Miyakawa revealed the involvement of Ca in the transmembrane-signaling system of a sex pheromene rhodotorucin A in Rhodosporidium toruloides a cells as a second messenger. He postulated the possible mechanism for the signal transduction of the pheromone including trigger peptidase, a pheromone receptor, Ca ATPase and Ca /calmodulin dependent protein kinase.
5. Tsuchiya and Miyakawa found that the oscillation of intracellular-free Ca concentration play a regulatory role on the progress of cell division cycle in S. cerevisiae.
6. Miyakawa and Fukui cloned the gene encoding rhodotorucin A and determined its DNA sequence.
7. Tsuchiya established the monoclonal antibody against the yeast nuclear proteins and identified a 70-Kd protein involved in the yeast DNA replication. P70 was found to have a molecular structure resembled to a heat shock protein, hsp70.
8. Tsuchiya and Fukui developed in vitro DNA uptake system of isolated yeast nuclei and found the requirement of ATPase and protein kinase in the trans-nuclear-membrane transport of plasmid DNA. They also identified a nuclear ATPase sensitive to WGA and suggested the involvement of the ATPase in nuclear transport system.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (65 results)

All Other

All Publications (65 results)

  • [Publications] I.Utatsu: J.Gen Microbiol.132. 1359-1365 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Kikuchi: Mol.Cell.Biol.6. 4053-4059 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Okazaki: Proc.Natl.Acad.Sci.USA. 83. 1232-1236 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Miyakawa: J.Gen.Microbiol.132. 1453-1457 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Miyakawa: J.Gen.Microbio.133. 439-443 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Miyakawa: J.Bacteriol.169. 1626-1631 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] I.Utatsu: J.Bacteriol.169. 5537-5545 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] I.Utatsu: Yeast. 4. 179-185 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Tachikawa: Agric.Biol.Chem.51. 1209-1210 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Miyakawa: J.Bacteriol.169. 1626-1631 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.K.Jeong: Eur.J.Biochem.169. 511-515 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] E.Tsuchiya: J.Bacteriol.170. 547-551 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Ushio: J.Ferment.Technol.66. 481-488 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Kamiryo: Yeast. 4. 277-285 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Miyakawa: J.Bacteriol.171. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] E.Tsuchiya: FEBS Letters.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 赤田倫治: 化学と生物. 26. 640-647 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 宮川都吉: 日本農芸化学会誌. 62. 1802-1804 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 宮川都吉: 化学と生物. 27. 20-27 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 上領達之: "酵母のバイオテクノロジー" 学会出版センター, (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 東江昭夫: "バイオ研究データブック酵母 編" 医学書院, (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 東江昭夫: "バイオテクノロジーレビュー" シー・エム・シー出版, (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 宮川都吉: "酵母の細胞工学と育種" 学会出版センター, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] I. Utatsu: "Functions encoded by the yeast plasmid pSB3 isolated from Zygosaccharomyces rouxii IFO 1730" J. Gen. Microbiol.132. 1359-1365 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y. Kikuchi: "A nuclear gene of Saccharomyces cerevisiae needed for stable maintenance of plasmids" Mol. Cell. Biol.6. 4053-4059 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Okazaki: "Two acyl-coenzyme A oxidases in peroxisomes of the yeast Candida tropicalis: Primary structures deduced from genetic DNA sequence" Proc. Natl. Acad. Sci., USA. 83. 1232-1236 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Miyakawa: "Involvement of Ca^<2+>/calmodulin in the sexual differentiation induced by mating pheromone rhodotorucine A in Rhodosporidium toruloides" J. Gen. Microbiol.132. 1453-1457 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Miyakawa: "Involvement of cell-surface proteins in sexual cell-cell interactions of Tremella mesenterica, a heterobasidiomycetous fungus" J. Gen. Microbiol.133. 439-443 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Miyakawa: "Purification and characterization of a Ca^<2+>-dependent emebranne peptidase Involved in the signaling of mating pheromone in Rhodosporidium toruloides" J. Bacteriol.169. 1626-1631 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] I. Utatsu: "Yeast plasmids resembling 2 um DNA: regional similarities and diversities at the molecular level" J. Bacteriol.169. 5537-5545 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] I. Utatsu: "Possible gene interchante between plasmid and chromosome in yeast" Yeast. 4. 179-185 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Tachikawa: "A rapid and transient increase of cellular Ca^<2+> in response to mating pheromone in Saccharomyces cerevisiae" Agric. Biol. Chem.51. 1209-1210 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y. K. Jeong: "Interaction with phospholipids of a membrane thiol peptidase that is essential for the signal transduction of mating pheromone in R. toruloides" Eur. J. Biochem.169. 511-515 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] E. Tsuchiya: "Characterization of a DNA uptake reaction through the nuclear membrane of isolated yeast nculei" J. Bacteriol.170. 547-551 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Ushio: "Construction of a hast-vector system in the osmophilic haploid yeast Zygosaccharomyces rouxii" J. Ferment. Technol.66. 481-488 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Kamiryo: "Expression and transport of Candida tropicalis peroxisomal acyl-CoA oxidase in the yeast Candida maltosa" Yeast. 4. 277-285 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Miyakawa: "Saccharomyces cerevisiae protein kinase dependent on Ca^<2+> and calmodulin" J. Bacteriol.171. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] E. Tsuchiya: "Identification of a wheat-germ-agglutinin sensitive ATPase in yeast nculei" FEBS Letters.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Ushio: J.Ferment.Technol.66. 481-488 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.kamiryo: Yeast. 4. 277-285 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Miyakawa: J.Bacteriol.171. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] E.Tsuchiya: FEBS Letters.

    • Related Report
      1988 Annual Research Report
  • [Publications] 赤田倫治: 化学と生物. 26. 640-647 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宮川都吉: 日本農芸化学会誌. 62. 1802-1804 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宮川都吉: 化学の生物. 27. 20-27 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 上領達之: "酵母のバイオテクノロジー" 学会出版センター, (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 東江昭夫: "バイオ研究データブック 酵母 編" 医学書院, (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 東江昭夫: "バイオテクノロジーレビュー" シー・エム・シー出版, (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宮川都吉: "酵母の細胞工学の育種" 学会出版センター, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] I. Utatsu: J. Bacteriol.169. 5537-5545 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] I. Utatsu: Yeast. 4. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] T. Tachikawa: Agric. Biol. Chem.51. 1209-1210 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] T. Miyakawa: J. Bacteriol.169. 1626-163 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Y. K. Jeong: Eur. J. Biochem.169. 511-515 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] E. Tsuchiya: J. Bacteriol.170. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] T. Kamiryo: J. Bacteriol.

    • Related Report
      1987 Annual Research Report
  • [Publications] M. Nakagawa: J. Bacteriol.

    • Related Report
      1987 Annual Research Report
  • [Publications] I.Utatsu: J.Gen.Microbiol.132. 1359-1365 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Y.Kikuchi: Mol.Cell.Biol.6. 4053-4059 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Okazaki: Proc.Natl.Acad.Sci.USA. 83. 1232-1236 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Miyakawa: J.Gen.Microbiol.132. 1453-1457 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Miyakawa: J.Gen.Microbio.133. 439-443 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Miyakawa: J.Bacteriol.169. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Tachikawa: J.Bacteriol.

    • Related Report
      1986 Annual Research Report
  • [Publications] E.Tsuchiya: Mol.Cell.Biol.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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