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Histochemical studies of wood formation and decomposition using microspectropgotometry.

Research Project

Project/Area Number 61440016
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 林産学
Research InstitutionHokkaido University

Principal Investigator

FUKAZAWA Kazumi  Fac. of Agri. Hokkaido Univ. Professor, 農学部, 教授 (40001408)

Co-Investigator(Kenkyū-buntansha) UJIIE Masao  Fac. of Agri. Hokkaido Univ. Professor, 農学部付属演習林, 教授 (20001422)
TERASAWA Minoru  Fac. of Agri. Hokkaido Univ. Professor, 農学部, 教授 (50003124)
SASAYA Takashi  Fac. of Agri. Hokkaido Univ. Professor, 農学部, 教授 (80001410)
TAKABE Keiji  Fac. of Agri. Hokkaido Univ. Instructor, 農学部, 助手 (70183449)
OHTANI Jun  Fac. of Agri. Hokkaido Univ. Assoc. Professor, 農学部, 助教授 (30001465)
香山 彊  北海道大学, 農学部, 教授 (60101135)
今川 一志  北海道大学, 農学部, 助手 (30002059)
Project Period (FY) 1986 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1987: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1986: ¥19,000,000 (Direct Cost: ¥19,000,000)
KeywordsWoody Cell wall / Microspectrophotometry / Lignin / Ultraviolet microscope / Fluorescence microscope / Wood chemical component / 木材成分 / 組織化学 / 堆積 / 分解 / セルロース / ヘミセルロース
Research Abstract

Results during the 4 years of this project were printed for a report of 209 pares and 10 chapters. The following is a summary of the report. 1. A microspectrophotometer (Carl Zeiss UMSP 80) was installed during the term of project. 2. Experimental methods of histochemical studies of wood substances using the UMSP 80 were investigated and made a manual for researchers (Chap.1-3.), which is very urgent problem for boundary studies between wood anatomy and chemistry. A part of the text will be published as one section in a book of "Methods in lignin chemistry" in print in U.S.A. 3. Deposition of cell wall components and heterogeneity in formation of lignin were investigated into following xylems ; (1) differentiating xylem in Hardwoods, (2) compression wood, (3) an increment of Buna (Chap.4). 4. Heterogeneity of lignin in tropical hardwoods differed from temperate hardwoods in many respects (Chap.5). 5. Cytological studies using UMSP was effective for the investigation of cell wall occurring in protoplast and callus (Chap.6). 6. Delignification processes from the cell wall of wood fiber were observed by the UMSP, and a series of this studies by Kayama et al. was awarded a Japanese TAPPI prize (Chap.7). 7. An analysis of colored substances in heartwood and wound tissue was made preliminarily (Chap.8). 8. Lignins in fiber screleids of Karamatsu was analyzed by histochemical methods (Chap. 9). 9. Distribution of inorganic constituents in a tree was discussed histochemically (Chap.10).

Report

(5 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (57 results)

All Other

All Publications (57 results)

  • [Publications] Takabe,K,M.Fujita,H.Harada and H.Saiki: "Lignification process in Cryptomeria(Cryptomeria Japonica D.Don)tracheid:Electron microscopic observation of lignin skeleton of differenciating xylem." Res.Bull.College Exp.For.Hokkaido Univ.43. 783-766 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takabe,K,and H.Harada: "Polysaccharide deposition during tracheid wall formation in Cryptomeria." Mokuzai Gakkaishi. 32. 763-769 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Terashima,N.,K.Fukushima,S.Tsuchiya and K.Takabe: "Heterogeneity in formation of lignin.Vll.An Autoradiographic study on the formation of guaiacyl and syringyl lignin in Poplar." J.Wood Chem.Technol.6. 495-504 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Terashima,N.,K.Fukushima and K.Takabe: "Heterogeneity in formation of lignin.Vlll.An Autoradiographic study on the formation of guaiacyl and syringrl lignin in Magnolia Kobus DC." Holzforschung. 40(Suppl). 101-105 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takabe K.and K.Fukazawa: "Cell wall formation of compression wood in Abies sachalinensis." Proceedings of IUFRO-IAWA Meeting“From cell wall formation to timber behaviour"Paris. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kojima Y.,S.R.Yoon and T.Kayama: "Improvement of physical properties of hardwood CTMP by ozone treatment." Proceedings of the International Seminar on Non conventional Pulping and Bleaching,Hamburg. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小沢修二,笹谷宜志: "トドマツの抽出成分(第4報)トドマツ材からのラクト-ル環を有する新シクロリグナン" 木材学会誌. 34. 169-175 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小島康夫,尹承洛,香山彊ほか: "広葉樹CTMPの製造に関する研究(第1報)サルファイト系前処理CTMPの物理的特性" 紙パ技術協会誌. 42. 49-60 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小島康夫,尹承洛,香山彊: "広葉樹CTMPの製造に関する研究(第3報)CTMP-O_3プロセスにより製造したパルプの特性評価" 紙パ技術協会誌. 42. 53-62 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 深沢和三: "顕微分光による細胞壁の検索" 木材学会誌. 34. 641-646 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kojima Y.,S.R.Yoon and T.Kayama: "Delignification from the cell wall of wood fibers with ozone." Mokuzai Gakkaishi. 34. 697-701 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 尹承洛,小島康夫,香山彊: "広葉樹CTMPのオゾン処理後の抽出処理によるパルプ特性の変化" 日本木材学会北海道支部講演集. 20. 45-50 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Terashima,N.,K.Fukushima,Y.Sano and K.Takabe: "Heterogeneiry in formation of lignin X.Visuarization of lignification process in differentiating xylem of pine by microautoradiography." Holzforschung. 42. 347-350 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 尹承洛,小島康夫,香山彊: "高収率パルプの紫外線顕微鏡観察" 北大農演研報. 46. 405-424 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 呉晋,深沢和三,大谷諄: "UV顕微分光法による数種雲南材のリグニン分布" 日本木材学会北海道支部講演集. 21. 61-64 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kudoh H.,S.Takikawa and M.Ujiie: "Rooting of Hlba(Thujopsis dolabrata var.hondai)cutting in different cultural beds and growth of their rooted cutting in plantation" J.Jpn.For.Soc.71. 200-203 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takabe,K.,K.Fukazawa and H.Harada: "Deposition of cell wall components in conifer tracheid,In“Plant wall polymers"N.G.Lewis and M.G.Plaice ed." American Chemical Society Symposium Series.399,Ame.Chem.Soc.,Washington DC, 47-66 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukazawa K.: "Ultraviolet microscopy.In“Methods in lignin chemistry"C.W.Dence and S.Y.Lin ed." Springer Verlag.Berlin, inpress (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takabe, K., M. Fujita, H. Harada and H. Saiki: "Lignification process in Cryptomeria tracheid: Electron microscopic observation of lignin skeleton of differentiating xylem." Res. Bull. College Exp. For. Hokkaido Univ.43. 783-799 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takabe, K., and H. Harada: "Polysaccharide deposition during tracheid wall formation in Cryptomeria." Mokuzai Gakkaishi, 32:763-769(1986).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Terashima. N., K. Fukushima, S. Tuchiya and K. Takabe: "Heterogeneity in formation of lignin. VII. An autoradiographic study on the formation of guaiacyl and syringyl lignin in Poplar." J. Wood Chem. Technol., 6:495-504(1986).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Terashima. N., K. Fukushima and K. Takabe: "Heterogeneity in formation of lignin. VIII. An autoradiographic study on the formation of guaiacyl and syringyl lignin in Magnolia Kobus DC." Holzforschung, 40(Suppl) ;101-105(1986).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takabe K. and K. Fukazawa: "Cell wall formation of compression wood in Abies sachalinensis." Proceedings of IUFRO-IAWA Meeting "From cell wall formation to timber behavior" Paris, (1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kojima Y., S. R. Yoon and T. Kayama: "Improvement of physical properties of hardwoods CTMP by ozone treatment." Proceedings of the International Seminar on Nonconventional Pulping and Bleaching, Hamburg, (1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ozawa S. and T. Sasaya: "Extractives of Todomatsu Abies sachalinensis Masters IV. New cyclolignans containing a lactol ring from the wood of Abies sachalinensis." Mokuzai Gakkaishi, 34(2)169-175(1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kojima Y., S. R. Yoon, T. Kayama, M. Horino and M. Takeda: "A study of production of CTMP from hardwood Part 1. Physical properties of CTMP produced from sulfitepenetrated hardwoods chips." Japan TAPPI 42(3)49-60(1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kojima Y., S. R. Yoon and T. Kayama: "A study of production of CTMP from hardwood Part 3. Characterization of pulps produced by CTMP-O_3 process." Japan TAPPI 42(10)53-62(1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukazawa K.: "Topochemistry of cell wall with a Microspectrophotometer." Mokuzai Gakkaishi, 34(8)641-646(1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kojima Y., S. R. Yoon and T. Kayama: "Delignification from the cell wall of wood fibers with ozone." Mokuzai Gakkaishi, 34(8)697-701(1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoon S. R., Y. Kojima and T. Kayama: "Changes of the pulp quality by extraction treatment after ozonation of CTMP from hardwoods." Proceedings of the Hokkaido Branch of the Japan Wood Res. Soc. 20:45-50(1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Terashima, N., K. Fukushima, Y. Sano and K. Takabe: "Heterogeneity in formation of lignin. X. Visualization of lignification process in differentiating xylem of pine by microautoradiography." Holzforschung, 42:347-350(1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoon S. R., Y. Kojima and T. Kayama: "Ultraviolet microscopic studies on fibers in high yield pulps." Res. Bull. of College Exp. For. Hokkaido Univ., 46(2)405-424(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Wu J., K. Fukazawa and J. Ohtani: "Lignin analysis in some tropical hardwoods in Yunnan using ultraviolet microscopy." Proc. Hokkaido Branch, Japan Wood Res. Soc., 21:61-64(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kudoh H., S. Takikawa and M. Ujiie: "Rooting of Hiba (Thujopsis dolabrata var. hondai) cuttings in different cultural beds and growth of their rooted cuttings in plantation." J. Japan For. Soc. 71(5)200-203(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takabe K., K. Fukazawa and H. Harada: "Deposition of cell wall components in conifer tracheids." "Plant wall polymers" N. G. Lewis and M. G. Plaice ed., American Chem. Soc. Symposium Series 399, Ame. Chem. Soc. Washington. 47-66 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukazawa K.: "Ultraviolet microscopy." "Methods in lignin chemistry" C. W. Dennce and S. Y. Lin ed. Springer Verlag. Berlin. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 尹承洛,小島康夫,香山彊: "広葉樹CTMPのオゾン処理後の抽出処理によるパルプ特性の変化" 日本木材学会北海道支部講演集. 20. 45-50 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Terashima,N.,K.Fukushima,Y.Sano and K.Takabe: "Heterogeneity in formation of lignin.X.Visuarization of lignification process in differentiating xylem of pine by microautoradiography." Holzforschung. 42. 347-350 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 尹承洛,小島康夫,狭山彊: "高収率パルプの紫外線顕微鏡観察" 北大農演研報. 46. 405-424 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 呉晋,深沢和三,大谷諄: "UV顕微分光法による数種雲南材のリグニン分布" 日本木材学会北海道支部構演集. 21. 61-64 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kudoh H.,S.Takikawa and M.Ujiie: "Rooting of Hlba(Thujopsis dolabrate var.hondai)cuttings in different cultural beds and growth of their rooted cuttings in plantation." J.Jpn.For.Soc.71. 200-203 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takabe,K.,K.Fukazawa and H.Harada: "Deposition of cell wall components in conifer tracheid,In“Plant wall polymers"N.G.Lewis and M.G.Plaice ed." American Chemical Society Symposium Series 399,Ame.Chem.Soc.,Washington DC, 47-66 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fukazawa K.: "Ultraviolet microscopy.In“Methods in lignin chemistry"C.W.Dence and S.Y.Lin ed." Springer Verlag.Berlin, inpress (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小島康夫,尹承洛,香山彊: 木材学会誌. 34. 697-701 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 小島康夫,尹承洛,香山彊: 紙パ技協誌. 42. 53-62 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 高部圭司,深沢和三,寺島典二,福島和彦: 第32回リグニン討論会講演要旨集. 1-4 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Keiji Takabe; Kazumi Fukazawa: IUFRO-IAWA Meeting ″From Cell wall Formation to Timber Behaviour″. (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 小島康夫,〓承洛,香山彊,堀野政司,竹田昌史: 紙パ技協誌. 42. 275-286 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Y. Kojima; S. R. Yoon; T. Kayama: Presented at the International Seminar on Nonconventional Pulping and Bleaching, Hamburg. (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 小島康夫,〓承洛,香山彊: 木材学会誌.

    • Related Report
      1987 Annual Research Report
  • [Publications] 小澤修二,笹谷宜志: 木材学会誌. 34. 169-175 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 高部圭司,深沢和三: 第31回リグニン討論会講演要旨集. 1-4 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 竹田昌文,寺沢実,香山彊: 第31回リグニン討論会講演要旨集. 5-8 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 高部圭司,宮内孝治,深沢和三: 第37回日本木材学会大会発表. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 三浦清,西口恭彦,香山彊,藤川清三: 第37回日本木材学会大会発表. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 今川一志: 第37回日本木材学会大会発表. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 尹承洛,小島康夫,香山彊: 第37回日本木材学会大会発表. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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