| Project/Area Number |
61440036
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Chiba University |
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Principal Investigator |
TANIGUCHI Masaru Chiba Univ. Sch. Med., Professor, 医学部, 教授 (80110310)
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| Co-Investigator(Kenkyū-buntansha) |
TAGAWA Masatoshi Chiba Univ. Sch. Med., Research Associate, 医学部, 助手 (20171572)
TOKUHISA Takeshi Kobe Univ. Sch. Med., Professor, 医学部, 教授 (20134364)
KANNO Masamoto Chiba Univ. Scho. Med., Research Associate (40161393)
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| Project Period (FY) |
1986 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥27,100,000 (Direct Cost: ¥27,100,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1987: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1986: ¥12,000,000 (Direct Cost: ¥12,000,000)
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| Keywords | Suppresser T cell receptor / T cell repertoire / Homogenous TCR usage / Valpha14 TCR gene family / TCRalpha-chain / Self reactive repertoire / 免疫系ネットワ-ク / 抑制T細胞リセプター / α鎖遺伝子 / トランスジェニック・マウス / 免疫応答調節 / 抑制T細胞レセプター / V_α遺伝子ゲノム塩基配列 / Antiーsense RNA / 抗原レセプター / 内部抗原 / キメラ・マウス |
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| Research Abstract |
We investigated T cell receptor (TCR) alpha-chain genes on keyhole limpet hemocyanin (KLH) specific suppresser T cells and obtained the following results.
1. New Valpha14 gene family was identified and isolated. 2. By using Valpha14 probe, Southern blot analysis showed that laboratory inbred mouse strains are divided into 3 groups by EcoRI digestion: C57BL group with 11.2 kb EcoRI fragment, most of other mouse strains with 2.0 Kb EcoRI fragment and DBA group with both 11.2 kb and 2.0 kb. Wild mice from Asia are similar to C57BL whereas European wild mice show the pattem similar to those with 2.0 kb or 2.0 + 11.2 kb. Thus, Valpha14 is a useful probe identify mouse origin. 3. Valpha14+alpha-chain associated with Jalpha281 and one nucleotide N-region is dominated in normal spleen and thymus. 4. The frequency of V14J281 alpha-chain in spleen is 1.5% of total alpha-chain, suggesting that this homogenous TCR alpha-chain is considered to be due to the positive selection. 5. The ligand of this alpha-chain seems to be an undefined self molecule which possesses cross-reactive epitome to KLH, and is expressed in mouse subspecies manner. 6. The selection of T cells bearing this alpha-chain is occurred both in thymus and extrathymic sites.
The above results suggest that regulatory T cells belong to the autoreactive T cell repertoire and maintain self tolerance as a fail-safe mechanisms.
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