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Reversibility and irreversibility of shock organ and its preservation for organ transplantation

Research Project

Project/Area Number 61440067
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

MIYAZAKI Masao  Kyoto Prefectural University of Medicine, Dept. of Anesthesiology. Prof., 医学部, 教授 (30079933)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Satoru  Kyoto Prefectural University of Medicine, Dept. of Assistant, 医学部, 助手 (90167578)
HOSOKAWA Toyoshi  Kyoto Prefectural University of Medicine, Dept. of Assist. Prof., 医学部, 講師 (80165555)
OKUDA Chieko  Kyoto Prefectural University of Medicine, Dept. of Assist. Prof., 医学部, 講師 (70079937)
柴 禄郎 (柴 祿郎)  京都府立医科大学, 医学部, 助手 (70178896)
大畑 恵美子  京都府立医科大学, 麻酔学教室, 助手 (70191947)
李 光喜  京都府立医科大学, 麻酔学教室, 助手 (50167053)
光藤 努  京都府立医科大学, 麻酔学教室, 講師 (20128721)
Project Period (FY) 1986 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥22,100,000 (Direct Cost: ¥22,100,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥16,000,000 (Direct Cost: ¥16,000,000)
KeywordsShock / Irreversibility / Transplantation / MOF / Ischemia / Reperfusion / TRH / Push-pull method / コリン・アセチルトランスフェラ-ゼ / 脳内糖値 / 脳内乳酸値 / 脳内芳香族アミノ酸 / Dichloroacetate / 腎機能 / 免疫抑制 / ウリナスタチン / モノクローナル抗体 / 二重染色法 / Thyrotropin-releasing hormone / 麻酔薬 / 臓器可逆性 / ショック / ニフェディピン / ニカルジピン / ATP / DCA / 腎血液量 / 虚血心 / 心原性ショック / イソフルレン / PG【I_2】 / 冠血流量 / 出血性ショック
Research Abstract

1. The purpose of the research The problems related to brain death and following organ transplantation are the states of other organs such as heart, kidney, liver, and endocrine and metabolic systems. Decision of brain death itself must be resurveyed from the stand point of brain metabolism and endocrine activity. If multiple organ failure occurred with brain death, transplantation of such failured organ will be meaningless. Investigation of shock organ, development of MOF,and brain endocrine system and metabolism during pre&ih brain death is the purpose of this project.
2. Results (1) Quantitative evaluation of irreversibility of shock Quantitative evaluation of irreversibility of shock in each organs and systems were done by animal experiments and clinical survey of shock cases. Quantitative representation is shown on each categories and indices such as clinical circulatory system, myocardial functions, blood gases, acid-base balances, metabolism, electrolytes, endocrine systems, and … More reticuloendotlierial system. Those figures are displayed on the table, and this will be very useful indices to evaluate the status of each organs and whole body condition. (2) Studies on reversibility and irreversibility of each organs, such as brain, heart, and kidney The effects of ischemia and postischemic reperfusion on brain cholinergic system of the rat and the effects of hyperoxia on central cholinergic system were investigated measuring activities of choline acetyltransferase, ACHE, and muscarinic ACh receptor. Suppression by ischemia and increase by hyperoxia were noted. Thyrotropin-releasing hormone (TRH) was investigated in detail on its cardiovascular effects and the role in hemorrhagic and endotoxin shock. The mechanism of its actions is summarized as follows. (1) The cardiovascular stimulating actions induced by centrally administered TRH are increase of sympathetic stimulation. (2) Presser effects of TRH are biptiasic and fast and continuous effects were noted. (3) Cholinergic, GABAergic, and dopaminergic central pathways are involved in the Presser action of TRH. (4) TRH increased in septum after hemorrhage, and high concentration of K increase TRH release dose-dependently. Stimulation of hemorrhage causes membrane depolarization of TRH contained nerve terminal and thus release of TRH increases. The effects of PGI_2 on coronary circulation in cardiogenic shock was studied together with the effect of PGE_1. Effect of arterial hypotension on canine circulating blood volume was calculated. The effects of nifedipine, nicardipine, ATP, and dichloroacetate on ischemic kidney were examined and their various effects were measured on renal blood flow and renal functions. Immunological and aminoacids measurement were also done to define reversibility. Less

Report

(5 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (55 results)

All Other

All Publications (55 results)

  • [Publications] MIYAZAKI,Masao et al: "Pathophysiology of circulatory shock:An overview" Magnesium. 8. 163-178 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MIYAZAKI,Masao et al: "Three approaches to pathophysiology of shock" Journal of Anesthesia. 2. 258-271 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] OKUDA,Chieko et al: "Determination of endogenous and systemically administered thyrotropin-releasing hormone(TRH)in push-pull perfusate of the fourth ventricle of conscious and pentobarbital-anestheized rats." Ann.N.Y.Acad.Sci.553. 514-516 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] OKUDA,Chieko et al: "Involvement of thyrotropin-releasing hormone in central regulation of cardiovascular system after bleeding in conscious rats." Brain Research. 474. 399-402 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] HASHIMOTO,Satoru et al: "Effect of adenosine triphosphate on canine renal circulation." J.Cardiovascular Pharmacology. 11. 204-208 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MIZOBE,Toshiki et al: "Changes in brain thyrotropin-releasing hormone in reversible and irreversible hemorrhagic shock in the rat." Circulatory shock. 26. 245-256 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MIYAZAKI,Masao: "Influence and use of anesthetics on the cardiovascular system during shock and trauma.In:Cardiovascular actions of anesthetics and drugs used in anesthesia.Vol.2" Karger,Basel,Switzerland, 294 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 宮崎正夫: "研修医のための蘇生法のおける最新薬物療法" 克誠堂, 184 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MIYAZAKI, Masao: "Influence and use of anesthetics on the cardiovascular system during shock and trauma." Cardiovascular actions of anesthetics and drugs used in anesthesia.Volume 2. Karger, Basel, Switzerland. 294 222-243 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MIYAZAKI, Masao et al: "Pathophysiology of circulatory shock: An overview." Magnesium. 8(3-4). 163-178 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] OKUDA, Chieko et al: "Determination of endogenous and systematically administered thyrotropin-releasing hormone (TRH) in push-pull perfusate of the fourth ventricle of conscious and pentobarbital-anesthetized rats." Ann. N.Y. Acad. Sci., 553: 514-516, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MIYAZAKI, Masao et al: "Three approaches to pathophysiology of shock." J. Anesthesia, 2(2):258-271, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] OKUDA, Chieko et al: "Involvement of thyrotropin-releasing hormone in central regulation of the cardiovascular system after bleeding in conscious rats." Brain research, 474(2): 399-402, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] OKUDA, Chieko et al: "Cardiovascular effect of intravenously administered thyrotropin-releasing hormone and its concentration in push-pull perfusion of the fourth ventricle in conscious and pentobarbital-anesthetized rats." Life Science, 42(11): 1181-1188, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] OKUDA, Chieko et al: "Effect of hypothermia on thyrotropin-releasing hormone content in the rat brain." Pharmacol. Biochem. behav., 30(4): 941-944, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] HASHIMOTO, Satoru et al: "Effect of adenosine triphosphate on canine renal circulation." J. Cardiovascular Pharmacol. 11(2): 204-208, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] TANAKA, Hiroshi et al: "Suppression of the Presser effect of centrally administered thyrotropin-releasing hormone under halothane, pentobarbital and flunitrazepam anaesthesia." Acta Anaesthesiol. Scand. 32(7): 535-540, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MIZOBE, Toshiki et al: "Changes in brain thyrotropin-releasing hormone in reversible and irreversible hemorrhagic shock in the rat." Circulatory shock 26(3): 245-256, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] SHIGEMI, Kenji: "Vascular responses to hypercapnia in anesthetized dogs." J. Anesthesia, 2(1): 1-7, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] HOSOKAWA, Toyoshi: "Effects of anesthesia and surgery on cell-mediated immunity -The application of two color analysis to classify functional lymphocyte subpopulation." Japanese Anaesthesia Journal's Review 2(4): 207-209, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] YUKIOKA,Naohiko: "Factors affecting renal hemodynamics in hypotensive anesthesia and shock." J. Kyoto Pref. Univ. Med. 97(4): 443-452, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] OKUDA, Chieko et al: "The involvement of central cholinergic mechanisms in cardiovascular responses in intracerebroventricular and intravenous administration of thyrotropin-releasing hormone." Life Science, 40(13): 1293-1299, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MITSUFUJI, Tsutomu et al: "The effects of isoflurane and sevoflurane on renal circulations." J. Kyoto Pref. Univ. Med. 96(4): 417-423, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MIYAZAKI, Masao et al: "Organ transplantation and reversibility and irreversibility of shock organ - With its prevention (Short middle report of Grant-in Aid for Scientific Research (A) 1986-1989 (IN Japanese)" Pathophysiology 8(2): 175-178, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] MIYAZAKI, Masao et al: Humoral effects of ATP, nicardipine and propranolol in trimethaphan induced hypotension and comparison of nicardipine and nifedipine. Proc. 4th Int. Sympo. on Endocrinology in Anesthesia and Surgery.Elsevier, Amsterdam, Netherlands, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] HOSOKAWA, Toyoshi et al: Interaction between immunological change and serum cortisol concentration in patients undergoing major and minor surgery.Elsevier, Amsterdam, Netherlands, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyazaki,Masao et al: "Pathophysiology of circulatory shock:An overview" Magnesium. 8. 163-178 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Miyazaki,Masao et al: "Three approaches to pathophysiology of shock" Journal of Anesthesia. 2. 258-271 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Miyazaki,Masao et al: "Humoral effects of ATP,nicardipine and propranolol in trimethaphan induced hypotension and comparison of nicardipine and nifedipine" Proc.4th Int.Sympo.on endocrinology in anesthesia and surgery. 4. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Okuda,Chieko et al: "Determination of endogenous and systemically administered thyrotropin-releasing hormone(TRH)in push-pull perfusate of the fourth ventricle of conscious and pentobarbital-anesthetized rats." Ann.N.Y.Acad.Sci.553. 514-516 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hosokawa,Toyoshi et al: "Interaction between immunological change and serum cortisol concentration in patients undergoing major and minor surgery" Proc.4th Int.Sympo.on endocrinology in anesthesia and surgery. 4. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大畑恵美子: "実験的腎虚血におけるDichloroacetateの腎保護作用" 京都府立医科大学雑誌. 98. 1177-1189 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 宮崎正夫: "研修医のための蘇生法における最新薬物療法" 克誠堂, 184 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 宮崎正夫: "図説救急医学講座4.ショックと急性臓器不全(杉本侃編)" メジカルビュ-社, 260( 12) (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大畑恵美子,柴祿郎,李光喜,宮崎正夫: Shock. 3. 88-89 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 堀義幸,細川豊史,宮崎正夫: 麻酔. 37. S344 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 細川豊史,堀義幸,中川美穂,宮崎正夫: 麻酔. 37. S346 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 佐和貞治,田中宏,奥田千恵子,溝部俊樹,出村久子,宮崎正夫: 麻酔. 37. S39 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 奥田千恵子,溝部俊樹,田中宏,佐和貞治,出村久子,宮崎正夫: 麻酔. 37. S226 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Toshiki Mizobe;Chieko Okuda: Circrlatory Shock. 26. 245-256 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宮崎正夫: "昭和62年度研究報告集 心大血管手術中およびショック時の心機能と腎肝血液動態の関係(軽度心原性ショックを伴う実験的虚血心の冠循環に対するイソフルレンとブロスタグランジンI_2の影響)" 国立循環器病センター, 352 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] MIYAZAKI, Masao: Journal of Anesthesia. 2.

    • Related Report
      1987 Annual Research Report
  • [Publications] SUGAI, Yoriko, MIYAZAKI, Masao: Acta Anaesthesiologica Scandinavica. 31. 224-226 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] SUGAI, Yoriko, MIYAZAKI, Masao: Europian Journal of Anaesthesia. 4. 161-166 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] HASHIMOTO, Satoru, MIYAZAKI, Masao: Circulatory Control - Cardiovascular Anesthesiology. 8 SUP. 278-279 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] LEE, Mitsuyoshi, MIYAZAKI, Masao: Circulatory Control - Cardiovascular Anesthesiology. 8 SUP. 270-271 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 李 光喜: 麻酔. 36. 933-939 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 宮崎 正夫: "昭和61年度 研究報告集 心大血管手術中およびショック時の心機能と腎肝血液動態の関係(ショック対照人為低血圧の研究" 国立循環器病センター, 621 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 李光喜: 麻酔. 36. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 光藤努: 京都府立医科大学雑誌. 95. 457-462 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 光藤努: 京都府立医科大学雑誌. 95. 463-469 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 奥田千恵子,溝部俊樹,宮崎正夫: 病態生理. 6. 111-116 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 奥田千恵子: Shock. 1. 37-52 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Chieko OKUDA;Masao MIYAZAKI,et al: J.Pharmacobio-Dyn.9. 98 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Masao Miyazaki: "Cardiovascular Actions of Anesthetics and Drugs Used in Anesthesia.vol.2.Influence and Use of Anesthetics on the Cardiovascular System during Shock and Trauma" Karger,Basel,Switzerland, 294(21) (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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