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Studies on sensorineural hearing loss using animal models

Research Project

Project/Area Number 61440072
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionUniversity of Tokyo

Principal Investigator

NOMURA Yasuya  Dept. of Otolaryngology, University of Tokyo Professor, 医学部, 教授 (30009948)

Co-Investigator(Kenkyū-buntansha) SUGASAWA Masashi  Dept. of Otolaryngology University of Tokyo Assistant, 医学部, 助手 (00179110)
KITAMURA Ken  Dept. of Otolaryngology University of Tokyo Assistant Professor, 医学部, 講師 (90010470)
MIZUNO Masahiro  Dept. of Otolaryngology University of Tokyo Associate Professor, 医学部, 助教授 (10049059)
KURATA Takeshi  Dept. of Pathology National Institute of Health Director, 病理部, 部長 (50012779)
船井 洋光  東京大学, 医学部, 講師 (90142249)
原 誠  東京大学, 医学部, 助手 (10173072)
Project Period (FY) 1986 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥29,000,000 (Direct Cost: ¥29,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1987: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1986: ¥16,000,000 (Direct Cost: ¥16,000,000)
Keywordsanimal model / perilymphatic fistula / vestibular function / Argon laser / otolithic organs / viral labyrinthis / electrophysiology / morphology / 内リンパ水腫 / コルチリンパ水腫 / 岬角電気刺激検査 / ウイルス / 突発性難聴 / レーザー / モデル動物 / 内耳破壊 / 聴神経単一活動電位
Research Abstract

A small amount of perilymph was suctioned from the scala tympani through the round window. Morphological findings are identical to those found in animals in which artificial perilymph was injected into the subarachnoid space in order to produce experimental perilymphatic fistula. Perilymph suctioning through the round window can be used as an animal model of perilymphatic fistula (PLF).
Using the animal model, changes of compound action potentials (CAP), cochlear microphonics (CM) and summating potentials (SP) were recorded. Two animals out of 17 showed slight elevation of thresholds of CM and CAP without change of SP polarity. No significant morphological changes were found in these animals. Three animals developed reversal of SP polarity. One of them became deaf after showing a high amplitude SP. Bulging of Reissner's membrane was observed in this animal. SP disappeared in the remaining 12 animals. Various changes were observed in the temporal bone of animals, such as, rupture, collap … More se of Reissner's membrane and loss of hair cells. It is concluded that rapid withdrawal of perilymph can produce pathological changes in the membranous labyrinth.
Vestibular function was examined in animals with PLF (n=67). Five hours after suctioning of perilymph (4-10 mul) from the scala tympani through the round window, the suctioned animals showed spontaneous nystagmus to the normal side (89.5%). The nystagmus disappeared within 24 hours. One week after surgery, 24.6% of the animals showed positional nystagmus to the lesion side. There was no ice-water caloric response in the lesion side (73.9%).
Application of argon laser to the inner eat will play an important role in treatment of inner ear diseases. Argon laser was irradiated to the otolithic organs of guinea pigs and cynomolgus monkeys after stapedectmy. After irradiation the stapes was replaced. It was found that a single irradiaon of 1.5w x 0.5s to the maculae utriculi and sacculi resulted in complete disappearance of the otolith, sensory cells and nerve fibers. Hearing was preserved in these animals. Caloric response to ice-water showed that the lateral ampulla was normal.
We have developed a multi-purposes evoked response recording system using a personal computer. Parameters, such as, sound pressure of stimulus (0-155 dB), frequency, wave forms (tone burst, click), and sampling time (10 msec-1 sec) can be easily controlled. Using the system it is now possible to measure and record rapid to slow evoked responses.
Surgical closure of perilymphatic fistula is required. As a material for this purpose, we use an recently found material "Beschitin". The material dissolves itself within the vestibule without marked tissue reaction. Less

Report

(5 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Nomura Y,Hara M,Okuno T: "Application of argon laser to the inner ear" Acta Otolaryngol(Stockh). 105. 439-444 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nomura Y,Hara M,Kurata T: "Experimental herpes simplex virus and cytomegalovirus labyrinthitis" Acta Otolaryngol(Stockh)Suppl. 457. 57-66 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Okuno T,Nomura Y,Hara M: "Partial labyrinthectomy with argon laser" Acta Otolaryngol(Stockh)Suppl. 456. 111-116 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Funai T,Hara M,Nomura Y: "An electrophysiologic study of experimental perilymphatic fistula" Am J Otolaryngol. 9. 244-255 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nomura Y: "Human temporal bone studies in Japan" Ann Otol Rhinol Laryngol Suppl 143. 98. 15-17 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hara M,Nomura Y,Saito K: "Histopathological study of the perilymph-suctioned labyrinth" Ann Otol Rhinol Laryngol(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nomura Y,Hara M,Okuno T,Yagi M,Kurata T,Nariuchi H: "Meniere's Disease" Kugler & Ghedini, 568 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nomura Y, Hara M, Okuno T: "Application of argon laser to the inner ear." Acta Otolaryngol (Stockh). 105. 439-444 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nomura Y, Hara M, Kurata T: "Experimental herpes simplex virus and cytomegalovirus labyrinthitis." Acta Otolaryngol (Stockh) Suppl 457: 57-66, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Okuno T, Nomura Y, Hara M: "Partial labyrinthectomy with argon laser." Acta Otolaryngol (Stockh) Suppl 456: 111-116, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Funai T, Hara M, Nomura Y: "An electrophysiologic study of experimental perilymphatic fistula." Am J Otolaryngol 9: 244-255, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nomura Y: "Human temporal bone studies in Japan." Ann Otol Rhinol Laryngol 98; Suppl: 15-17, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hara M, Nomura Y, Saito K: "Histopathological study of the perilymph-suctioned labyrinth." Ann Otol Rhinol Laryngol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nomura Y, Hara M, Okuno T, Yagi M, Kurata T, Nariuchi H: Meniere's Disease.Kugler & Ghedini, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nomura Y.,Hara M.,Okuno T.: "Application of argon laser to the inner ear" Acta Otolaryngol(Stockh). 105. 439-444 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nomura Y.,Hara M.,Kurata T.: "Experimental herpes simplex virus and cytomegalovirus labyrinthitis" Acta Otolaryngol(Stockh)Suppl. 457. 57-66 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Okuno T.,Nomura Y.,Hara M.: "Partial labyrinthectomy with argon laser" Acta Otolaryngol(Stockh)Suppl. 456. 111-116 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Funai T.,Hara M.,Nomura Y.: "An electrophysiologic study of experimental perilymphatic fistula" Am J Otolaryngol. 9. 244-255 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nomura Y.: "Human temporal bone studies in Japan" Ann Otol Rhinol Laryngol Suppl 143. 98. 15-17 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hara M.,Nomura Y.,Saito K.: "Histopathological study of the perilymph-suctioned labyrinth" Ann Otol Rhinol Larygol.

    • Related Report
      1989 Annual Research Report
  • [Publications] Nomura Y.,Hara M.,Okuno T.,Yagi M.,Kurata T.,Nariuchi H.: "Meniere's Disease" Kugler 〓 Ghedini, 568 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Okuno,T;Nomura,Y;Hara,M: Acta Otolaryngol. Suppl456. 111-116 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Funai,H;Hara,M;Nomura,Y: Am J Otolaryngol. 9. 244-255 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Funai,H;Hara,M;Nomura,Y: Acta Otolaryngol. Suppl456. 92-97 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 野村恭也,奥野妙子,原誠,八木昌人,倉田毅: Ear Research Japan. 20. (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nomura,Y;Harada,T;Hara,M: ORL. 150. 201-211 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nomura Y; Hara M; Okuno t: Acta Otolaryngology. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nomura Y; Hara M; Kurata T: Acta Otolaryngology. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nomura y; Harada i; Hara M: ORL. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 奥野妙子,野村恭也,原誠: Ear Research Japan. 19. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 菅沢正,仙波哲雄,室伏利久,野村恭也,森浩一: Ear Research Japan. 19. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 原誠: 耳鼻咽喉科展望. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nomura Y;Hara M: Am J Otolaryngol. 7. 267-275 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 原誠,野村恭也,船井洋光: Ear Research Japan. 18. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 船井洋光,原誠,野村恭也: Ear Research Japan. 18. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Nomura Y;Hara M;Funai H;Okuno T: Acta Otolaryngology. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Funai H;Hara M;Nomura Y: Am J Otolaryngol.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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