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Regulation of initiation of chromosomal replication in bacteria and a study on its regulation.

Research Project

Project/Area Number 61440092
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionOsaka University, Medical School

Principal Investigator

YOSHIKAWA Hiroshi  Osaka Univ., Medical School, Professor, 医学部, 教授 (70019876)

Co-Investigator(Kenkyū-buntansha) MORIYA Shigeki  Osaka Univ., Medical School, Assistant, 医学部, 助手 (40191051)
OGASAWARA Naotake  Osaka Univ., Medical School, Lecturer, 医学部, 講師 (10110553)
Project Period (FY) 1986 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥32,200,000 (Direct Cost: ¥32,200,000)
Fiscal Year 1989: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1988: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1987: ¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 1986: ¥17,000,000 (Direct Cost: ¥17,000,000)
KeywordsDnaA protein / DnaA-box / Bacillus subtilis / Pseudomonas putida / Micrococcus luteus / Mycopurasuma capricorum / Chromosomal replication / DnaAーbox / シュードモナス / 染色体の複製開始 / シュードモナス菌 / dnaA遺伝子 / oriC / 細菌染色体 / 複製開始 / DnaA遺伝子 / DnaA・Box配列 / 大腸菌 / P.putida / 塩基配列
Research Abstract

1. Conservation of DnaA protein and DnaA-box in bacteria and their evolution (Yoshikawa): (1) Conservation of the combination of dnaA gene and DnaA-boxes was confirmed in Pseudomonas and Micrococcus in addition to E. coli and B. subtilis, suggesting its central role in initiation of chromosomal replication in bacteria. (2) We succeeded in cloning a dnaA homologue from Mycoplasma, cells containing the smallest genome, suggesting ubiquitous presence of DnaA protein in enbacteria. (3) A clone containing a possible dnaA homologue was obtained from yeast, a lower eukaryote.
2. Function of DnaA protein and regulation of its expression (Moriya): (1) Involvement of DnaA protein and DnaA-box in initiation of replication and its regulation was demonstrated using a dnaA-ts mutant cell of B. subthis. (2) DnaA protein was purified, and the mode of its specific binding to DnaA-box was analyzed in vitro. (3) An anti-DnaA antibody was prepared to determine cellular content of DnaA which determines the initiation frequency under certain physiological conditions. (4) In steady state growth, other factors seem to be limiting in the initiation in addition to DnaA.
3. Structure and function of replication machinery in B. subtilis (Ogasawara): (1) Structure of B. subtilis dnaG and dnaH genes were determined to identify them as homologous wtih E. coli dnaN and dnaZX, respectively. They code for two subunits of polymerase III. (2) Structure of a second gene for initiation of replication, dnaB, was determined. Its product protein was purified, and an antibody was prepared. So far, DNA binding activity of the protein was not detected in vitro.

Report

(5 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (60 results)

All Other

All Publications (60 results)

  • [Publications] Ogasawara,N.: "A Bacillus subtilis dnaG mutant harbours a mutation in a gene homologous to the dnaN gene of Escherivhia coli." Gene. 45. 227-231 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara,N.: "Nucleotide sequence and organization of dnaB gene and neighbouring genes on the Bacillus subtilis chromosome." Nucleic Acids Res.14. 9989-9999 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara,N.: "Genes and their organization in the replication origin region of the Bacillus subtilis chromosome: Comparison with the Escherichia coli chromosome." Adv.Biophys.21. 19-33 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara,N.: "Organization of genes and regulatory sequences in the origin region of the Bacillus subtilis chromosome: Remarkable homology with Escherichia coli." Bacillus molecular genetics and biotechnology applications(ed.Hoch,J.and Ganesan,A.T.)Academic Press Inc.335-352 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小笠原直毅: "原核生物の染色体複製開始の調節機構." 細胞工学. 6. 6-15 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 吉川寛: "染色体複製開始領域の共通構造." 代謝. 24. 467-477 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Andrup,L.: "Interaction of the Bacillus subtilis DnaA-like protein with the Escherichia coli DnaA protein." J.Bacteriol.170. 1333-1338 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Moriya,S.: "Regulation of initiation of chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome." EMBO J.7. 2911-2917 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Moriya,S.: "DnaA-boxes in the oriC region of the Bacillus subtilis chromosome are involved in regulation of initiation of chromosomal replication." Genetics and biotechnology of Bacilli,2.(eds.Ganesan,A.T.and Hoch,J.A.)Academic Press Inc.275-280 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fujita,M.Q.: "Structure of dnaA region of Pseudomonas putida: Conservation among three bacteria,Bacillus subtilis,Escherichia coli and P.putida," Mol.Gen.Genet.215. 381-387 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 吉川寛: "DNA複製" DNA複製とその調節(吉川寛、関口睦夫、内田久雄編)丸善. 1-6 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小笠原直毅: "細菌染色体の複製調節" DNA複製とその調節(吉川寛、関口睦夫、内田久雄編)丸善. 117-137 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小笠原直毅: "染色体複製開始の制御;枯草菌DnaA蛋白質の機能とその発現の制御" 蛋白質・核酸・酵素. 34. 1135-1148 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 吉川寛: "DNA複製から細胞複製へ" 病態生理. 9. 161-163 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara,N.: "Comparative anatomy of oriC of eubacteria." The bacterial chromosome(eds.Rilley,M.and Drlica,D.)Am.Soc.Microbiol.287-295 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukuoka,T.: "Purification and characterization of an initiation protein for chrmosomal replication,DnaA,in Bacillus subtilis." J.Biochem.(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fujita,M.Q.: "Structure of dnaA region of the Micrococcus luteus chromosome: Comparison among eubacteria." Gene(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Moriya,S.: "Isolation of a dnaA mutant defective in initiation of replication in Bacillus subtilis: Amount of DnaA protein determines cell's initiation potential." EMBO J.(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara, N., Moriya, S., Mazza, G. and Yoshikawa, H.: "A Bacillus subtilis dnaG mutant harbors a mutation in a gene homologous to the dnaN gene of Escherichia coli." Gene. 45. 227-231 ((1986))

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara, N., Moriya, S., Mazza, P. G. and Yoshikawa, H.: "Nucleotide sequence and organization of dnaB gene and neighboring genes on the Bacillus subtilis chromosome." Nucleic Acids Res., 14, 9989-9999 (1986).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara, N. and Yoshikawa, H.: "Genes and their organization in the replication origin region of the Bacillus subtilis chromosome: Comparison with the Escherichia coli chromosome." Adv. Biophys., 21, 19-33 (1986).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara, N., Moriya, S. and Yoshikawa, H.: "Organization of genes and regulatory sequences in the origin region of the Bacillus subtilis chromosome: Remarkable homology with Escherichia coli." Bacillus molecular genetics and biotechnology applications (ed. by Hoch, J. and Ganesan, A. T.) Academic Press, 335-352 (1986).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Andrup, L., Atlung, T., Ogasawara, N., Yoshikawa, H. and Hansen, F.G.: "Interaction of the Bacillus subtilis DnaA-like protein with the Escherichia coli DnaA protein." J. Bacteriol., 170, 1333-1338 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Moriya, S., Fukuoka, T., Ogasawara, N. and Yoshikawa, H.: "Regulation of initiation of the chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome." EMBO J., 7, 2911-2917 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Moriya, S., Fukuoka, T., Ogasawara, N. and Yoshikawa, H.: "DnaA-boxes in the oriC region of the Bacillus subtilis chromosome are involved in regulation of initiation of chromosomal replication." Genetics and biotechnology of Bacilli, 2 (ed. by Ganesan, A. T. and Hoch, J. A.) Academic Press, 275-180 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fujita, M. Q., Yoshikawa, H. and Ogasawara, N.: "Structure of dnaA region of Pseudomonas putida: Conservation among three bacteria, Bacillus subtilis, Escherichia coli and P. putida." Mol. Gen. Genet., 215, 381-387 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara, N. Fujita, M. Q., Moriya, S., Fukuoka, T., Hirano, M. and Yoshikawa, H.: "Comparative anatomy of oriC of eubacteria." The bacterial chromosome (ed. by Riley, M. and Drlica, D.) Am. Soc. Microbiol., 287-295 (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukuoka, T., Moriya, S., Yoshikawa, H. and Ogasawara, N.: "Purification and characterization of an initiation protein for chromosomal replication, DnaA, in Bacillus subtilis." J. Biochem., (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fujita, M. Q., Yoshikawa, H. and Ogasawara, N.: "Structure of dnaA region of the Micrococcus luteus: Comparison among eubacteria." Gene, (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Moriya, S., Kato, K., Yoshikawa, H. and Ogasawara, N.: "Isolation of a dnaA mutant defective in initiation of replication in Bacillus subtilis: Amount of DnaA protein determines cell's initiation potential." EMBO J., (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ogasawara,N.: "A bacillus subtilis dnaG mutant harbours a mutation in a gene homologous to the dnaN gene of Escherivhia coli." Gene. 45. 227-231 (1986)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ogasawara,N.: "Nucleotide sequence and organization of dnaB gene and neighbouring genes on the Bacillus subtilis chromosome." Nucleic Acids Res.14. 9989-9999 (1986)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ogasawara,N.: "Genes and their organization in the replication origin region of the Bacillus subtilis chromosme:Comparison with the Escherichia coli chromosome." Adv.Biophys.21. 19-33 (1986)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ogasawara,N.: "Organization of genes and regulatory sequences in the origin region of the Bacillus subtilis chromosome:Remarkable homology with Esherichia coli." Bacillus molecular genetics and biotechnology applications.(ed.Hoch,J.and Ganesan,A.T.)Academic Press Inc.335-352 (1986)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小笠原直毅: "原核生物の染色体複製開始の調節機構." 細胞工学. 6. 6-15 (1987)

    • Related Report
      1989 Annual Research Report
  • [Publications] 吉川寛: "染色体複製開始領域の共通構造." 代謝. 24. 467-477 (1987)

    • Related Report
      1989 Annual Research Report
  • [Publications] Andrup,L.: "Interaction of the Bacillus subtilis DnaA-like protein with the Escherichia coli DnaA protein." J.Bacteriol.170. 1333-1338 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Moriya,S.: "Regulation of initiation of chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome." EMBO J.7. 2911-2917 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Moriya,S.: "DnaA-boxes in the oriC region of the Bacillus subtilis chromosome are involved in regulation of initiation of chromosomal replication." Genetics and biotechnology of Bacilli,2.(eds.Ganesan,A.T.and Hoch,J.A.)Academic Press Inc.275-280 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fujita,M.Q.: "Structure of dnaA region of Pseudomonas putida:Conservation among three bacteria,Bacillus subtilis,Escherichia coli and P.putida." Mol.Gen.Genet.215. 381-387 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 吉川寛: "DNA複製." DNA複製とその調節(吉川寛、関口睦夫、内田久雄編)丸善. 1-6 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小笠原直毅: "細菌染色体の複製調節." DNA複製とその調節(吉川寛、関口睦夫、内田久雄編)丸善. 117-137 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小笠原直毅: "染色体複製開始の制御;枯草菌DnaA蛋白質の機能とその発現の制御." 蛋白質・核酸・酵素. 34. 1135-1148 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 吉川寛: "DNA複製から細胞複製へ." 病態生理. 9. 161-163 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ogasawara,N.: "Comparative anatomy of oriC of eubacteria." The bacterial chromosome(eds.Rilley,M.and Drlica,D.)Am.Soc.Microbiol.287-295 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fukuoka,T.: "Purification and characterization of an initiation protein for chrmosomal replication,DnaA,in Bacillus subtilis." J.Biochem.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fujita,M.Q.: "Structue of dnaA region of the Micrococcus luteus chromosome:Comparison among eubacteria." Gene. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Moriya,S.: "Isolation of a dnaA mutant defective in initiation of replication in Bacillus subtilis:Amount of DnaA protein determines cell's initiation potential." EMBO J.1990.

    • Related Report
      1989 Annual Research Report
  • [Publications] Andrup,L.: Journal of Bacteriology. 170. 1333-1338 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Moriay,S.: The EMBO journal. 7. 2911-2917 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fujita,M.: Molecular and General Genetics. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shigeki Moriya: Genetics and Biotechnology of Bacilli.2. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 吉川 寛: 代謝. 24. 467-477 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 小笠原 直毅: 細胞工学. 6. 6-15 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Naotaka Ogasawara: Gene. 45. 227-231 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Naotaka Ogasawara: Nucleic Acids Research. 14. 9989-9999 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 小笠原直毅: 細胞工学. 6. 6-15 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 吉川寛: 遺伝. 40. 5-13 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Naotaka Ogaswara: "Bacillus Molecular Genetics And Biotechomology Applycations" Academic Press Inc., 497 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 吉川寛: "バイオサイエンスのための新しい分子遺伝学" 南江堂, 257 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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