| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Research Abstract |
1) Lypid analogous compounds having nucleicacid bases or nucleosides, such as N,N-distearoyloxyethyl-3-(thymine-1-yl)propionamide, 2',3'-di-O-palmitoyluridine-5'-phosphocholine, 2',3'-di-O-stearoyl-5-fluorouridine, and 2',3'-di-O-stearoyl-adenosin e-5h-phosphocholine, were synthesized and the formation of lyposomes, their gel-liquid crystalline phase transition temperature, and their enthalpy change in phase transition were studied. They formed monomolecular membranes, but not multilayer membrane, because of their poor hydrophobicity. Monomolecular layer of lypids having uridine showed an interaction with nucleotides and polynucleotides dissolved in water.
2) Uridine derivatives having two long chain alkyl esters which contain polymerizable diacetylene unit were synthesized.
3) Dicarboxylic acid derivative of nucleic acid bases were polycondensed with diamine in the presence of polymers having nucleic acid bases as side groups. When the base have complementary interaction, a significant selectivity of base pairs were observed as increase of the rate and enriched contents of complementary bases.
4) Dicarboxylic acid derivatives and long chain alkyl esters of linked two nucleic acid bases were synthesized.
5) Large ring lypid derivatives, such as 2-hexadecanoyl-sn-glycero(3) dotriacontamethy-lene(3)-2-hexadecanoyl-sn-glycero(1)phosphocholine and 2-acetyl-3-(cholinephospho)-sn-glycero(1)dotriacontamethylene(1)-2-acetyl sn-glycero(3)phosphocholine were synthesized, and their mrmbrane formation was surveyed.
6) Hydrolysys of lyposome by phospholypase A_2 was studied.
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