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Cycloheximide-resicetance gene isolated from Candida maltosa : Mechanism of action and its utilization

Research Project

Project/Area Number 61470129
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research InstitutionFaculty of Agriculture, The University of Tokyo

Principal Investigator

TAKAGI Masamichi  Faculty of Agriculture, The University of Tokyo, 農学部, 助教授 (50018339)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥3,600,000 (Direct Cost: ¥3,600,000)
KeywordsCandida maltosa / Saccharomyces cerevisiae / cycloheximide / gene cloning / protein syntesis / ribosome modification / Saccharomyses cerevisiae / リボソーム / 抗生物質耐性 / 耐性化遺伝子 / クローニング
Research Abstract

1. We isolated a gene designated the RIM-C gene (ribosome-modification by cycloheximide) from the genome of Candida maltosa by using a host-vector system of Sachharomyces cerevisiae, which is sensitive to cycloheximide (CYH). When this gene was introduced to S. cerevisiae, its ribosomes were modified to be resistant to CYH.
2. We determined the nucleotide sequence of DNA region containing the RIM-C gene.
3. By using the dot-hybridization technique, we found that the modified ribosomes did not contain a RNA product of the gene. An RNA product of this gene was found to contain poly(A)-tail. These data indicate that the gene encode a mRNA and not a new species of rRNA.
4. By using the two-dimensional gel electrophoresis, we did not found a new ribosomal protein in the modified ribosomes. It is suggested that the gene does not code for a new ribosomal protein, but codes for a modification enzymes of a component of ribosomes.
5. We determined the transcription strand of the gene and the transcription start-point.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 高木正道他: J. Gen. Appl. Microbiol. 31. 267-275 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 高木正道: J. Bacteriol. 168. 417-419 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 高木正道: J. Bacteriol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masamichi,Takagi et al.: "Induction of cycloheximide resistance in Candida maltosa by modifying the ribosomes" J. Gen. Appl. Microbiol.31. 267-275 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masamichi,Takagi et al.: "Cloning in Saccharomyces cerevisiae of a cycloheximide resistance gene from the Candida maltosa which modifies ribosomes" J. Bacteriol.168. 417-419 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masamichi,Takagi et al.: "Transcription analysis of the RIM-C (ribosome-modification by cycloheximde) gene isolated from Candida maltosa" J. Bacteriol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 高木正道 他: J.Gen.Appl.Microbiol.31. 267-275 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 高木正道 他: J.Bacteriol.168. 471-419 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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