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Studies on Paratropomyosin which Contributes to Meat Tenderization

Research Project

Project/Area Number 61470140
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 畜産化学
Research InstitutionHokkaido University

Principal Investigator

TAKAHASHI Koui  Faculty of Agriculture, Hokkaido University, 農学部, 助教授 (40001432)

Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsTenderization of Meat / Aging of Meat / Paratropomyosin / Actin-Myosin Interaction / Weakening of Rigor Linkages / 硬直結合の脆弱化 / サルコメアの伸長 / パラトロポミオシンの精製 / 硬直結合
Research Abstract

1. Paratropomyosin inhibited the Mg-ATPase activity and enhanced K-ATPase activity of myofibrils stoichiometrically, and its maximal binding to actin was estimated to occur at a molar ratio of 1:13. These results indicate that paratropomyosin is able to bind to thin filaments of myofibrils, and that due to its greater affinity for the myosin binding site on actin, paratropomyosin competes for the binding site and helps weaken rigor linkages formed between actin and myosin.
2. Intact myofibrils stained with fluorescent antibodies against paratropomyosin showed that paratropomyosin was exclusively located at the A-I junction of sarcomeres. In stretched myofibrils (3.7 mum in sarcomere length), the approximate width of the fluorescent stripes and their relation to the A band remained constant. During postmortem storage of muscles, on the other hand, paratropomyosin was translocated from its original position at the A-I junction onto thin filaments. The translocation of paratropomyosin was … More successfully induced with a calcium ion concentration of 0.1 mM in the presence of protease inhibitors.
3. The rigor tension of glycerinated fibers was reduced to about 65% of the initial value within 10 min after the addition of purified paratropomyosin, whereas it remained constant for at least 3.5 hr in control fibers. Paratropomyosin showed a stronger effect on the increase in sarcomere length of passively stretched fibers, which developed weaker tensions. The most successful rigor solution contained 0.2-0.25 M KC1, pH 5.5, at 5-10゜C. Under these conditions, which coincide well those of postmortem muscles, the sarcomere length was easily increased from 2.4 to 3.6 mum, if rigor-contracted fibers were passively stretched after the addition of purified paratropomyosin. We therefore conclude that in postrigor muscles, paratropomyosin is released from the A-I junction region following the increase in the sarcoplasmic calcium ion concentration to 0.1 mM, and then binds to thin filaments, which results in weakening of rigor linkages formed between actin and myosin. Less

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] 高橋興威: 肉の科学. 26. 93-101 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Takahashi: J.Biochem.102. 1187-1192 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Hattori: J.Biochem.103. 809-814 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Yamanoue: J.Biochem.103. 843-847 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 高橋興威: 生物科学. 40. 140-417 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Takahashi: J.Biochem.105. 149-151 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koui Takahashi: J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 中江利孝 編: "乳・肉・卵の科学" 弘学出版, 295 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koui Takahashi: "Meat Tenderization during Postmortem Ageing" Niku no Kagaku. 26. 93-101 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koui Takahashi: "Paratropomyosin, a New Myofibrillar Protein, Weakens Rigor Linkages Formed between Actin and Myosin" J. Biochem.102. 1187-1192 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Akihito Hattori: "Localization of Paratropomyosin in Skeletal Muscle Myofibrils and Its Translocation during Postmortem Storage of Muscles" J. Biochem.103. 809-814 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Minoru Yamanoue: "Effect of Paratropomyosin on the Increase in Sarcomere Length of Rigor-Shortened Skeletal Muscles" J. Biochem.103. 843-847 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koui Takahashi: "Recent Studies on Skeletal Muscles" Seibutsukagaku. 40. 140-147 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Masayuki Takahashi: "Ca^<2+>-Sensitive Transition in the Molecular Conformation of Molluscan Muscle Myosins" J. Biochem.105. 149-151 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koui Takahashi: " -Actinin is a Component of the Z Filament, a Structural Backbone of Skeletal Muscle Z-Disks" J. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koui Takahashi: Meat and Meat Products in "Science of Milk, Meat and Egg". Kogaku-Shuppan Inc.,52-77 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Hattori: J.Biochem.103. 809-814 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Yamanoue: J.Biochem. 103. 843-847 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 高橋興威: 生物科学. 40. 140-147 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Takahashi: J.Biochem. 105. 149-151 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Takahashi: J.Biochem.

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Wakahashi: J.Biochem.102. 1187-1192 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] A.Hattori: J.Biochem.

    • Related Report
      1987 Annual Research Report
  • [Publications] M.Yamanoue: J.Biochem.

    • Related Report
      1987 Annual Research Report
  • [Publications] 高橋興威: 肉の科学. 26. 93-101 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Takahashi: J.Biochemistry.

    • Related Report
      1986 Annual Research Report
  • [Publications] M.Yamanoue: J.Biochemistry.

    • Related Report
      1986 Annual Research Report
  • [Publications] 中江利孝 編著: "乳・肉・卵の科学" 弘学出版, 295 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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