| Project/Area Number |
61470141
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
畜産化学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
ADACHI Susumu College of Agriculture, Tohoku University, 農学部, 教授 (10005580)
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| Co-Investigator(Kenkyū-buntansha) |
山路 厚雄 東北大学, 農学部, 教務職員
SUGAWARA Hiroshi College of Agriculture, Tohoku University, 農学部, 教務職員 (80089797)
YAMAJI Atsuo College of Agriculture, Tohoku University
戸羽 隆宏 東北大学, 農学部, 助手 (10108483)
須山 享三 東北大学, 農学部, 助手 (70005635)
伊藤 敬敏 東北大学, 農学部, 助教授 (80005610)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Kefiran / Kefir / Capsular polysaccharide / Gelforming polysaccharide / 乳用乳酸菌 / 〓膜多糖 / ケフィール粒 / ケフイラン |
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| Research Abstract |
Lactobacillus kefiranofaciens sp. nov. is described. Four strains of this species isolated from kefir grains withthe modified KPL medium are slime-forming, homofermentative, rod-shaped lactic acid bacteria. They differ from all the validly descibed homofermentative species of the genus Lactobacillus in their carbohydrate fermentation pattern. Their guanine-plus-cytosine content of deoxyribonucleic acids obtained from six other Lactobacillus species do not show significant relatedness to a representative strain of the new species. The type strain of L. kefiranofaciens sp. nov. is WT-2B(ATCC 43761).
Comparative studies of polysaccharides from kefir grains were performed by gel filtration, sugar composition analysis and methylation analysis. The characteristics of the polysaccharides from kefir grains were similar to those of an encasuled monofermentative Lactobacillus species, but different from that of L. kefir. It is concluded that an encapsulad homofermentative Lactobacillus-species is mainly responsible for polysaccharide production in the grains.
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