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STUDY ON METHOD FOR AMINO ACID SEQUENCE OF SUB-PICO-MOLE PROTEIN

Research Project

Project/Area Number 61470160
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionSCIENCE UNIVERSITY OF TOKYO

Principal Investigator

TSUGITA Akira  DEPT. LIFE SCI., RES. INST. SCI. TECH. SCI. UNIV. TOKYO, PROF., 総合研究所生命科学研究部門, 教授 (00028284)

Co-Investigator(Kenkyū-buntansha) SATAKE Kazuo  DEPT. CHEM. FAC. SCI. SCT. UNIV. TOKYO, PROF., 理学部I部化学科, 教授 (20096704)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsN-terminal analysis / Sensitization with aminofluoresein / アミノフロレセイン / 蛍光末端分析 / 超微量アミノ酸配列 / 蛋白質N末端配列決定法 / 高感度化 / 0.1fモル蛍光アミノ誘導体 / ATZアミノ酸とアミノフロレセンの反応 / Bセル転写賦活因子 / DNAー蛋白複合体の配列決定法 / レーザ螢光検出 / 球型シーケンサ / SDSゲネ泳動の二重ゲル / 揮揆性緩衝液のゲル泳動 / C未酵素分解(微量)法の開発 / 500fモル / 螢光アミン誘導体
Research Abstract

N-terminal sequencing of sub-micro quantities of protein is one of the most demanded projects in the fields of cellular and molecular biology. We have developed a method for sensitizing ATZ-amino acids which are intermediate products of Edman degradation. Several sensitizing reagents involving, radioactive amines such as ^<125>I-iodohistamine and fluorescent amines were tested for their reaction efficiency, sensitivities of the products and their ease of handling. The most suitable reagent, aminofluorescein (AF), was chosen for further investigation. Commercial AF reagents were purified by high pressure liquid chromatography (HPLC). The reaction kinetics between AF and ATZ-Leu was surveyed for the optimum ratio of reagent, reaction temperature, and reaction time. ATZ-derivatives of 20 amino acids were tested for their reaction yields and the stability of the 20 different derivatives. Except for Glu, Arg and His(45%:, all amino acids gave guantitative yields and all 20 products were found to be stable. The AF derivatives showed an increase of more than 10 times sensitivity at pH 8 than at pH 5.0. this lead to the use of an alkalineinsensitive column for HPLC separation at pH 8. all 20 amino acid af derivatives were separated by HPLC and the sensitivity of the product was about 0.u fmole. The method was applied to a commercial protein sequencer with minor modifications to the program. Sequencing of 100f- 1 mpol of protein have been achieved for a standard protein and several unknown proteins, each 7 - 10 steps. 3 unexpected peaks were observed which derived from residual phenylisothiocyanate, trifluoroacetic acid and an impurity in AF. These are substantially reduced by changing the program and purifying the reagent. For further sensitization of the sequencer, several lines of fundamental experiments have been performed to established a new sequencer.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (109 results)

All Other

All Publications (109 results)

  • [Publications] K.Maeda;et al.: Eur.J.Biochem.154. 197-203 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Nozu;et al.: Plant Sci.44. 47-51 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] F.Gabrielli;et al.: Bull.Mol.Biol.Med.11. 57-66 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] F.Gabrielli:et al.: Mol.Cell.Biochem.71. 129-134 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] N.T.Tong;et al.: Biochem.Biophys.Acta.874. 296-304 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.W.Mewes;et al.: Biol.Chem.Hoppe Seyler. 368. 206 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: J.Prot.Chem.6. 121-130 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: Prot.Seq Data Anal.1. 99-102 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: J.Biochem.102. 1593-1597 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Kamo;et al.: J.Biochem.102. 243-246 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita: Proceeding of ROC-JAPAN symposium of biotechnology. 11. 74 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] P.Schrmann:et al.: Experientia. 44. A44 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Yokoi;et al.: J.Amer.Soc.Brewing Chem.46. 99-103 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: J.Biochem.103. 339-401 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.Nagashima;et al.: Prot.Seq.Data Anal.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Kamo;et al.: Prot.Seq.Data Anal.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Kamo;et al.: Prot.Seq.Data Anal.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Satake;et al.: Prot.Seg.Data Anal.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Jacq;et al.: J.Bact.in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Kamada;et al.: Chem.Pharm.Bull.46. 99-103 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: J.Prot.Chem.7. 316 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Kamo;et al.: Peptide Chemistry. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Nozu;et al.: Prot.Seg.Data Anal.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: J.Prot.Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 次田晧: 化学と生物. 26. 330-337 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 次田晧: 化学と生物. 26. 404-411 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 次田晧: 化学と生物. 26. 456-464 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] P.Shurmann;et al.: "Thioredoxin and Glutaredoxin Systems:Structure and Function Relationships between primary structure and function of Chloroplast thioredoxin" Raven Press, 31-38 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita: "Advances in Biophysics" Japan Scientific Societies press,Elsevier, 81-113 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: "Proteins:structure and function Approaches for microsequencing" Plenum Pub., 315-322 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: "Purefecation and characterization of micro quantities of protein Protein Structure Function Relationship Elsevier Pub.BV ed." Pergamon Press, 309-313 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: "Approaches for Microsequencing Proteins:structure and function" Plenum Pub., 315-322 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: "Strategies for the manipulation and sequencing of micro quantities of protein The Seventh seminar on Science and Technology;Mol.Biol."" 43-55 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Tsugita;et al.: "Protein structure-function relationship" Karachi Univ.Press, 6 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 次田晧: "タンパク質・ペプチドの高速液体クロマトグラフィー 超高感度ATZ-アミノ酸の誘導化" 化学同人, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Maeda + 4: "Further characterization and amino acid sequence of m-type thioredoxins from spinach chloroplasts" Eur. J. Biochem.154. 197-203 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y. Nozu + 1: "The amino acid sequence of cucumber mottle mosaic virus" Plant Sci.44. 47-51 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] F. Gabrielli + 1: "H1A and H1B histones of normal and cancer human cells; amino acid compositions of H1A and H1B histones purified by polyacrylamide gel electrophoresis" Bull. Mol. Biol. Med.11. 57-66 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] F. Gabrielli + 1: "H10 histiones of normal and cancer human cells" Mol. Cell. Biochem.71. 129-134 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] N.T. Tong + 2: "Purification and characterization of high molecular-mass forms of Achromobacter collagenase" Biochem. Biophys. Acta.874. 296-304 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.W. Mewes et al.: "Rapid hydrolysis of proteins and peptide by HCl-TFA vapor" Biol. Chem. Hoppe Seyler. 368. 206 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: "Approaches for sub-microsequencing" J. Prot. Chem.6. 121-130 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: "The amino acid sequence of crystalline sheets: a proteolytic fragment of the major head protein (gp23^*) of bacteriophage" Prot. Seq. Data Anal.1. 99-102 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: "Vapor-phase acid (HCl and TFA) hydrolysis of peptide and protein" J. Biochem.102. 1593-1597 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Kamo et al.: "Carboxypeptidase digestion in the presence of detergent" J. Biochem.102. 243-246 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita: "Approaches for sub-microsequencing" Proceeding of ROC-JAPAN symposium of biotechnology. 11. 74 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] P. Schrmann et al.: "The primary structure of spinach chloroplast thioredoxin F" Experientia. 44. 44 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Yokoi et al.: "Characterization of major proteins and peptides in beer" J. Amer. Soc. Brewing Chem.46. 99-103 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: "Sensitization of amino acid derivatives obtained from Edman degradation amino terminal sequencing with radioactive labelled iodohistamine" J. Biochem.103. 399-401 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H. Nagashima et al.: "Amino acid sequence of ferredoxin isolated from Cyanidium caldarium strain RK-1" Prot. Seq. Data Anal.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Kamo et al.: "Amino acid sequences of ferredoxins from rice cultivar, Japonica and Indica" Prot. Seq. Data Anal.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Satake et al.: "Hemerythrin from Lingula unguis consists of two different subunits, and" Prot. Seq. Data Anal.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: "Sensitization of Edman amino acid derivatives using the fluorescent reagent, 4-aminofluorescein" J. Biochem.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Jacq et al.: "Characterization of 12 KDa membrane protein" J. Bact.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Kamada et al.: "Generation of alpha and beta-kallikreins from porcine pancreatic prokallikrein by the action of trypsin" Chem. Pharm. Bull.46. 99-103 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: "Micro manipulation and micro-sequencing of proteins" J. Prot. Chem.7. 316 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Kamo et al.: "Sensitization of amino terminal sequencing of peptide" Peptide Chemistry. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y. Nozu et al.: "The amino acid sequence of plastocyanine from rice (japonica)" Prot. Seq. Data Anal.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: "Micro manupulation and micro sequencing of proteins" J. Prot. Chem.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita: "Analysis in submicroquantities protein 1. Purification of protein" Kagaku to Seibutsu. 26. 330-337 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita: "Analysis in submicroquantity protein 2. Amino acid composition" Kagaku to Seibutsu. 26. 403-411 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita: "Analysis in submicroquantity protein 3. Amino terminal sequencing" Kagaku to Seibutsu. 26. 456-464 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] P. Shurmann + 4: Theioredoxin and Glutaredoxin Systems: Structure and Function Relationships between primary structure and function of chloroplast thioredoxin. Raven Press New York, 31-38 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita: Advances in Biophysics. Japan Scientific Societies press, Elsevier, 81-113 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: Proteins: structure and function Approaches for microsequencing. Plenum Pub. Co. New York, 315-322 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: Purification and characterization of micro quantities of protein protein Structure Function Relationship Elsevier Pub. BV ed. by Zaidi, Z.H.Pergamon Press, 309-313 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: Approaches for Microsequencing Proteins: structure and function. Plenum Pub. Co. New York, 315-322 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: Strategies for the manipulation and sequencing of micro quantities of protein "The Seventh seminar on Science and Technology; Mol. Biol.". 43-55 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita et al.: Protein structure - function relationship. Karachi Univ. Press, 6 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Tsugita: Delivatization of Sub-micro quantity of ATZ-Amino Acid. Kagaku-Dojin HPLC of Protein and Peptide eds by T. Ikenaka and F. Sakiyama,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Yokio 他: J.Amer.Soc.Brewing.Chem.46. 99-103 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Tsugita 他: J.Biochem.103. 399-401 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.Nagashima 他: Prot.Seq.Data Anal.

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Kamo 他: Prot.Seq.Data Anal.

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Satake 他: Prot.Seq.Data Anal.

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Tsugita 他: J.Biochem.

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Jacq 他: J.Bact.

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Kamada 他: Chem.Pharm.Bull. 46. 99-103 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Tsugita 他: J.Prot.Chem.7. 316 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Kamo 他: Peptide Chemistry. (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Nozu 他: Prot.Seq.Data Anal.

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Tsugita 他: J.Prot.Chem.

    • Related Report
      1988 Annual Research Report
  • [Publications] 次田晧: 化学と生物. 26. 330-337 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 次田晧: 化学と生物. 26. 404-411 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 次田晧: 26. 1988. (456-464)

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Tsugita 他: "Purification and characterization of micro quantities of protein.Protien Structure Function Relationship Elsevier Pub.BV ed." Pergamon Press, 309-313 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Tsugita 他: "Approaches for Microsequencing Proteins:structure and function" Plenum Pub.Co.New York, 315-322 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Tsugita 他: "Strategies for the manipulation and sequencing of micro quantities of protein."The Seventh seminar on Science and Technology;Mol.Biol."" 43-55 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Tsugita 他: "Protein structure-function relationship" Karachi Univ.Press, 6 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 次田晧: "タンパク質・ペプチドの高速液体クロマトグラフィー超高感度ATZ-アミノ酸の誘導化" 化学同人, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] A.Tsugita: J.Prot.Chem. 6. 124-130 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] H.W.Mewes: Hoppe-Seyler's Z.Biochem. 367. 368 (1986)

    • Related Report
      1987 Annual Research Report
  • [Publications] A.Tsugita: J.Biochem. 102. 1593-1597 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] M.Kamo: J.Biochem. 102. 243-246 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] A.Tshugita: J.Biochem. 103. NO.3 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] A.Tsugita: Prot.Seq.Data Anal. 1. NO.2 (1987)

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  • [Publications] A.Tsugita: Proc.Roc-Japan. Ser1. 74 (1987)

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  • [Publications] S.Yokoi: J.Amer.Soc.Brewing Chem. (1988)

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  • [Publications] A.Tsugita: "(Approaches for microsequencing)Proc.Symposium of American Protein Chemist" Plenum Pub.Co New York, (1988)

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  • [Publications] A.Tsugita: "(Developements in Protein Microsequence)Advances in Biophysics,vol.23,23" Japan Scientific societies press Elsevier, 81-1 (1987)

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  • [Publications] K.Maeda: Eur.J.Biochem. 154. 197-203 (1986)

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  • [Publications] Y.Nozu: Plant Sci.44. 47-51 (1986)

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  • [Publications] F.Gabrielli: Bull.Mol.Biol.& Med.11. 56-66 (1986)

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  • [Publications] F.Gabrielli: Mol.Cell.Biol.71. 129-134 (1986)

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  • [Publications] N.T.Tong: Biochem.Biophys.Acta. 874. 296-304 (1986)

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  • [Publications] A.Tsugita: Protein Chemistry. 6. 121-133 (1987)

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  • [Publications] S.Yokoi: J.Amer.Soc.brewing chem.(1987)

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  • [Publications] A.Tsugita: "Approaches for microsequencing Proceeding of Symposium of Americn Proteinchemist ed by J.J.L′Italien" Plenum Pub.Co.New York, (1987)

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Published: 1987-03-31   Modified: 2016-04-21  

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