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Molecular Mechnisms of Meristematic Tissue-Specific Expression of Plnat Histone Genes Introduced by a Ti-Plasmid Vector, Especially concerning Cis-Acting Control Elements for the Initiation and Termination of

Research Project

Project/Area Number 61480007
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionHokkaido University

Principal Investigator

IWABUCHI Masaki  Faculty of Science, Hokkaido University, 理学部, 助教授 (30000839)

Co-Investigator(Kenkyū-buntansha) TABATA Tetsuya  Faculty of Science, Hokkaido University, 理学部, 助手 (10183865)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywords@heat histone genes / Ti-plasmid vector / Gene transfer / S phase-specific gene expression / Cis-acting control element / Trans-acting control factor / 転写調節シスエレメント / オクタマー配列 / DNA結合タンパク
Research Abstract

During the last two years, in order to study the molecular mechanisms of the transcriptional regulation of the genes which are specifically experssed at the S phase during cell cycle, we have examined the cis- and trans-acting Control elements of the histone genes using the cloned wheat histone H3 and H4 genes. To examine the transcriptional activity of the histone gene, the Ti-plasmid mediated gene transfer system was used; the wheat histone genes were introduced into sunflower hypocotyl cells via Ti-plasmid and the transcripts of the wheat genes in transformed cells were measured by the S1 assay.
The analysis of the transcriptional activities of the 5' deletion mutants of the H3 and H4 genes revealed that some cis-elements promoting a high efficiency of the transcription are located between the positions -668 and 191 and that the hexameric sequence just upstream from the octameric sequence which exists in all plant histone genes examined so far seemd to have some connection with the transcriptional regulation. On the other hand, the octameric sequence was not shown to be a motif to regulate the transcription of the histone genes.
Several experiments using the gel retardation assay, methylation interference assay and DNase I footprint method have demonstrated that there exist nuclear protein(s) specifically binding to the hexameric sequence in wheat cells. The nuclear protein Which was tentatively termed HBP-1 are more abundantly contained in germinating than dormant germs. Furthermore, in wheat seedling in which the cell cycle was partially synchronized by aphidicoline treatment, HBP-1 was persent in the highest concentration in the nuclear extract from an S phase-abundant cell population. These data have suggested that the hexameric sequence and HBP-1 are cis- and trans-acting control elements, respectively, involved in cell cycle-dependent transcription of the wheat histone genes.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Tabata, Tetsuya: Plant & Cell Phsiol.27. 929-933 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tabata, Tetsuya: Plant & Cell Physiol.28. 73-82 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mikami, Koji: FEBS Letters. 223. 273-278 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岩淵雅樹: 細胞工学. 5. 465-477 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岩淵雅樹: 蛋白質核酸酵素(別冊). 30. 207-216 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岩淵雅樹: 現代化学(増刊). (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 山口彦之(編集): "最新植物バイオテクノロジー要覧" R&Dプラニング社, (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tabata,Tetsuya: "Characteristic Sequences in the 3' Flanking Region of Wheat Histone Genes." Plant & Cell physiol.27. 929-933 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tabata,Tetsuya: "An Accurate Transcription of Wheat Histone Genes in Sunflower Cells." Plant & Cell Physiol.28. 73-82 (19887)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Mikami,Koji: "Nuclear Protein(s) Binding to the Conserved DNA Hexameric Spquence Postulated to Regulate Transcription of Wheat Histone Genes." FEBS Letters. 223. 273-278 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Iwabuchi,Masaki: "The Structure and Transcription of Wheat Histone Genes (In Japanease)." Saibokogaku. 5. 465-477 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Iwabuchi,Masaki: "Cell Cycle-Dependent Gene Expression System in Plants (In Japanease)." Tanpakushitsu Kakusan Koso. 30. 207-216 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tabata,Tetsuya: Plant Cell physiol.27. 929-933 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tabata,Tetsuya: Plant Cell Physiol.28. 73-82 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岩渕雅樹: 細胞工学. 5. 465-477 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岩渕雅樹: 蛋白質核酸酵素(別冊). (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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