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Functions of lipophilic quinones in photosynthetic reaction sytems of photosynthesis

Research Project

Project/Area Number 61480009
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionUniversity of Tokyo

Principal Investigator

KATOH Sakae  College of Arts and Sciences, University of Tokyo, 教養学部, 教授 (50011515)

Co-Investigator(Kenkyū-buntansha) SATOH Kazuhiko  College of Arts and Sciences, University of Tokyo, 教養学部, 助手 (00090522)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1987: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1986: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsPastoquinone / Photosystem II / Oxygen evolving preparation / Q_B-protein / DBMIB / Benzoquinone / Photoaffinity labeling / シネココッカス / ビタミン【K_1】 / 系【II】反応中心複合体 / 電子伝達体 / 系I反応中心複合体 / 光標識実験
Research Abstract

Functions of plastoquinone associated with PS II Preparations isolated from the thermophilic cyanobacterium Sybechococcus sp. were studied
1. Photoaffinty labeling of various PS II preparations clearly demonstrated for the first time that a phenol-type herbicide binds to the 28 KDa Q_B-protein and the 47 KDa chlorophyll-carrying protein has a plastoquinone binding site
2. Numbers and functions pf plastoquinone molecules associated with PS II preparetions were determined. Three quinone molecules present in the oxygen evolving preparations function as Q_A and Q_G and possibly on the water side of the reaction center of PS II
3. Chemical composition of the highly purified oxygen evolving comolexes was analyzed. The results show that two plastoquinone molecules, which function as Q_A and on the water side of PS II,are needed for oxygen evolution
4. A unigue mode of benzoquinone reduction was found in the oxygen evolving prepqrations. High rate of oxygen evolution was obtained with lipophilic benzoquinones, while less lipophilic molecules were poor electron acceptors. DCMU strongly inhibited reduction of all the electron acceptors examined. In contrast, DBMIB suppressed competitively reduction of benzoquinones but not that of ferricyanide and dichlorophenolindophenol. This suggests that benzoquinones bind to the Q_B-site, by replacing plastoquinone, and accept electrons directly from Q_A. The arrhenius plot of benzoquinone reduction suggests that a change in the conformation of the Q_B-protein occurs at 19゜C
5. Duroquinol oxidation by PS I in the cyanobacterial cells was also inhibited competitively by DBMIB, suggesting a common inhibition mechanism of DBMIB between the ozygen evolving complexes and the cytochrome b_6-f complexes.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Yuichro Takahashi: Biochimica et biophysica Acta. 848. 183-192 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kazuhiko Satoh: Biochimica et Biophysica Acta. 851. 202-208 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masaru Nanba: Biochimica et Biophysica Acta. 851. 484-490 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takashi Ohno: Biochimica et Biophysica Acta. 852. 1-8 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yuichiro Takahashi: Photosynthesis Research. 11. 29-36 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yuki Tanaka: Biochimica et Biophysica Acta.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yuichiro Takahashi: "Numbers and functions of Plastoquinone molecules associated with Photosystem II preparations from Synechococcus sp." Biochimica et Biophysica Acta. 848. 183-192 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kazuhiko Satoh: "Jerbicide and plastoquinone-binding proteins of Photosystem II reaction center complexes from the thermophilic cyanobacterium Synechococcus sp." Biochimica et Biophysica Acta. 851. 202-208 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Masaru Nanbe: "The site and mechanism of duroquinol oxidation by the cytochrome b_6-f complex in Synechococcus sp." Biochimica et Biophysica Acta. 851. 484-490 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takashi Ohno: "Chemical composition of qurified oxygen evolving complexes from the thermophilic cyanobacterium Synechococcus sp." Biochimica et Biophysica Acta. 852. 1-8 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yuichiro Takahashi: "Effect of theptane-ectraction on the stability of subunit organization of photosystem I reaction center complexes." photosynthetic Research. 11. 29-36 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yuichiro Takahashi: Eicchimica et Biophysica Acta. 848. 183-192 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kazuhiko Satoh: Biochimica et Biopnysica Acta. 581. 202-208 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Masaru Nanba: Biochimica et Biophysica Acta. 851. 484-490 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takashi Ohno: Biochim.Biophys.Acta. 852. 1-8 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Isao Enami: Plant ξ Cell Physiology. 27. 1395-1405 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yuichiro Takahashi: Photosynthesis Research. 11. 29-36 (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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