Analysis of the mechanisms of cell motility using living cells and cell-free systems.
| Project/Area Number |
61480019
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | The Open University of Japan (1987-1988)
Tokyo Institute of Technology (1986) |
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Principal Investigator |
HIRAMOTO Yukio The University of the Air, Professor, 教養学部, 教授 (50011440)
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| Co-Investigator(Kenkyū-buntansha) |
浜口 みやこ 東京工業大学, 理学部, 技官 (60192275)
HAMAGUCHI Yukihisa (HAMAGUCHI Miyako) Tokyo Institute of Technology, Associate Professor, 理学部, 助教授 (70016161)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Xenopus eggs / Sea urchin eggs / Mitotic apparatus / Mitotic spindle / Cvto-mechanical properties / カルシウム / 原形質の細胞外単離 / 原形質の力学的性質 / 遠心顕微鏡 / 細胞運動 / 細胞分裂 / 水晶体 / 原形質 / 星状体 |
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| Research Abstract |
1. Experimental systems for keeping organelle activities outside the cell. Cytoplasm at the clear zone of centrifuged xenopus eggs was isolated in paraffin oil and used it as a medium for keeping activities of organelles isolated from living cells. Cytoplasm of lens cells of fish eggs was tried to use for the same purpose.
2. Micromanipulation of the mitotic apparatus and its mechanical properties. Effects of deforming and severing the mitotic apparatus on the chromosome movement in sea urchin eggs were analyzed by micromanipulation. The results suggested that the motive force for anaphase chromosome movement is located at or near the moving chromosomes. The measuement of mechanical properties of the mitotic apparatus revealed that the equatorial region of the spindle is more fragile than the remaining regicons of the spindle.
3. Quantitative measurement of the spindle birefringence. A novel method was developed to measure total birefringence within a microscopic area having heterogeneous birefringence, and it was applied to measure the birefringence of the spindles in normal and Colcemid-treated sea urchin eggs.
4. Measurement of mechanical properties of the cytoplasm. Movements of gold particles, oil droplets or the nucleus in the cytoplasm by centrifugal force were observed in sea urchin and starfish eggs with a centrifuge microscope. It was found that the distribution and nature of the cytoskeleton in the cytoplasm. are closely related to the movement of the particles etc. Causal relations betweenthe position of the cleavage furrow and that of the mitotic apparatus were analyzed by moving the mitotic apparatus in the cell by centrifugation at various stages of mitosis.
5. Roles of calcium ions during cellular activity. Spatial and temporal changes in intracellular calcium ion concentration during fertilization in fish eggs, protoplasmic streaming of slime mold and movement of amoebae were determined by measuring the luminescence of aequorin-loaded cells.
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Report
(4 results)
Research Products
(21 results)
