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Analysis of the mechanisms of cell motility using living cells and cell-free systems.

Research Project

Project/Area Number 61480019
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionThe Open University of Japan (1987-1988)
Tokyo Institute of Technology (1986)

Principal Investigator

HIRAMOTO Yukio  The University of the Air, Professor, 教養学部, 教授 (50011440)

Co-Investigator(Kenkyū-buntansha) 浜口 みやこ  東京工業大学, 理学部, 技官 (60192275)
HAMAGUCHI Yukihisa (HAMAGUCHI Miyako)  Tokyo Institute of Technology, Associate Professor, 理学部, 助教授 (70016161)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsXenopus eggs / Sea urchin eggs / Mitotic apparatus / Mitotic spindle / Cvto-mechanical properties / カルシウム / 原形質の細胞外単離 / 原形質の力学的性質 / 遠心顕微鏡 / 細胞運動 / 細胞分裂 / 水晶体 / 原形質 / 星状体
Research Abstract

1. Experimental systems for keeping organelle activities outside the cell. Cytoplasm at the clear zone of centrifuged xenopus eggs was isolated in paraffin oil and used it as a medium for keeping activities of organelles isolated from living cells. Cytoplasm of lens cells of fish eggs was tried to use for the same purpose.
2. Micromanipulation of the mitotic apparatus and its mechanical properties. Effects of deforming and severing the mitotic apparatus on the chromosome movement in sea urchin eggs were analyzed by micromanipulation. The results suggested that the motive force for anaphase chromosome movement is located at or near the moving chromosomes. The measuement of mechanical properties of the mitotic apparatus revealed that the equatorial region of the spindle is more fragile than the remaining regicons of the spindle.
3. Quantitative measurement of the spindle birefringence. A novel method was developed to measure total birefringence within a microscopic area having heterogeneous birefringence, and it was applied to measure the birefringence of the spindles in normal and Colcemid-treated sea urchin eggs.
4. Measurement of mechanical properties of the cytoplasm. Movements of gold particles, oil droplets or the nucleus in the cytoplasm by centrifugal force were observed in sea urchin and starfish eggs with a centrifuge microscope. It was found that the distribution and nature of the cytoskeleton in the cytoplasm. are closely related to the movement of the particles etc. Causal relations betweenthe position of the cleavage furrow and that of the mitotic apparatus were analyzed by moving the mitotic apparatus in the cell by centrifugation at various stages of mitosis.
5. Roles of calcium ions during cellular activity. Spatial and temporal changes in intracellular calcium ion concentration during fertilization in fish eggs, protoplasmic streaming of slime mold and movement of amoebae were determined by measuring the luminescence of aequorin-loaded cells.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Takashi Iwamatsu: Developmental Biology. 125. 451-457 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yasiaki Yoshimoto: ptoceedings of the Japan Academy. 64. 109-112 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yukio Hiramoto: Cell Motility and the Cytoskeleton. 10. 172-184 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kiyoko Kuroda: Protoplasma. 144. 64-67 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Talashi Iwamatsu: Developmental Biology. 129. 191-197 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshitaro Nakamo: Zooiogical Science. 5. 539-544 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yukio Hiramoto 共著: "Eualuation ov cytomechanical properties" Springer-Verlag, 16 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yukio Hiramoto 共著: "Biomechamics of cell Division" Plenum, 20 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takashi Iwamatsu,: Developmental Biology. 125. 451-457 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yosuaki Yoshimoto,: Proceedings of the japan Academy. 64. 109-112 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yukio Hiramoto,: Cell Motility and the Cytoskeleton. 10. 172-184 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kiyoko Kuroda,: Protoplasma. 144. 64-67 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takashi Iwamatsu,: Developmental Biology. 129. 191-197 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshitaro Nakano,: Zoological Science. 5. 539-544 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yukio.Hiramoto: Cell Motility and the Cytoskeleton. 9. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yukio.Hiramoto: "Mechanical properties of the protoplasm of echinoderm eggs at various stages of cell cycle ″NATO ASI,Biomechanics of cell division″" Plenum,London, 374 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Miyako S.Hamaguchi;Yukihisa Hamaguchi;Yukio Hiramoto: Development,Growth and Differentiation. 28. 143-156 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Miyako S.Hamaguchi;Yukihisa Hamaguchi;Yukio Hiramoto: Developmental,Growth and Differentiation. 28. 461-470 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kiyoko Koroda;Noburo Kamiya;Yasuaki Yoshimoto;Yukio Hiramoto: Proceeding Japan Academy. 62B. 117-121 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 平本幸男,中野義太郎: 細胞. 18. 122-126 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yukihisa Hamaguchi;Masaru Toriyama;Hikoichi Sakai;Yukio Hiramoto: Cell Structure and Function. 11. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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