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Chemical structures of carbohydrate epitopes of host cell membrane recognized by fimbriae of pathogenic bacteria

Research Project

Project/Area Number 61480081
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionHokkaido University

Principal Investigator

NAIKI Masaharu  Faculty of Veterinary Medicine, Hokkaido University, 獣医学部, 助教授 (10020752)

Co-Investigator(Kenkyū-buntansha) ONO Eturo  Faculty of Veterinary Medicine, Hokkaido University, 獣医学部, 助手 (00160903)
YANAGAWA Ryo  Faculty of Veterinary Medicine, Hokkaido University, 獣医学部, 教授 (30001514)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsPathogenic E / coli / fimbrial vaccination / Diarrhea / Pyelonephritis / Crynebacterium / Gene recombination / Glycosphingolipid / 線毛抗原 / コリネバクテリューム / 牛腎孟腎炎 / 牛下痢症 / 単クローン抗体
Research Abstract

1. Uropathogenic E. coli P fimbriae -- E. coli with P fimbriae which recognize Gal -4Gal sequence of CTH(P^k antigen), globoside (P antigen) and P_1 glycolipid (P_1 antigen) were frequently isolated from human pyelonephitis urines. We identified that some E. coli strains with same P fimbrial antigen recognize Forssman glycolipid but not any Pantigenic glycolipid and other strains recognize potently Forssman glycolipid and weakly CTH and globoside. These fimbrial adhesin was different from P adhesin.
2. Enterotoxigenic E. coli K99 fimbrial--K99 fimbrial E. coli is a causative agent of calf and piglet diarohea. This fimbrial adhesin was found to recognize N-glycolylneuraminyl-lactosylceramide, GM_3(NeuGc) of host intestinal mucosa. GM_3(NeuGc) was detected in intestinal mucosa of newborn calf and piglet but it completely disappeared at the 2nd week after birth. The adhesin factor was concentrated in recombinant bacteria by recombination DNA technique. It became applicable for the fimbrial vaccination.
3. Corynebacterium renale fimbriae--C. renale is a causative agent of bovine pyelonephritis. The bacteria adhere to urinary track epitherium with their fimbriae. We prepared monoclonal antibodies to the fimbriae, which can inhibit the adhesion to host cells. The monoclonal antibody recognized 48000 fimbrial protein. Recobination DNA technique alsp showed that 1.4K bp of C. renale chromosomal DNA codes the same protein product.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Ono,E.: Infection and Immunity. 39(3). (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Orino,K.: Infection and Immunity.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kubota,T.: Jpn.J.Vet.Sci.,. 50. 199-207 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koga,T.: Jpn.J.Vet.Sci.,. 50. 1277-1278 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kudo,Y.: Vet.Microbiol. 13. 75-85 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ito,H.: Vet.Microbiol. 14. 165-171 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 内貴正治: "細胞工学「糖脂質の細胞工学的応用」" 秀潤社, 664-669 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yanagawa,R: "Progress in Veterinary Microbiology and Immunology 「Causative agents of bovine pyel」nephritis COrynebacterium renale,C.pilosum and C.cystitidis" S.Kargar, 158-174 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ono, E.: "Ganglioside epitope recognized by k99 fimbriae from enterotoxigenic Escherichia coli" Infection and Immunity. 39(3). (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Orino,K.: "Two kinds of P-fimbrial variants of uropathogenic Escherichia coli recognizing Forssman glycosphingolipid" Infection and Immunity. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kubota, T.: "Phagocytosis of piliated and non-piliated Corynebacterium renale by murine polymorphonuclan leukocytes" Jpn. J. Vet. Sci.,. 50. 199-207 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koga, T.: "Population shift between piliated and non-piliated bacteria in various culture conditions of Corynebacterium renale" Jpn. J. Vet. Sci.,. 50. 1277-1278 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kudo. Y.: "Isolation and characterization of monoclonal antibodies against pili of Corynebacterium renale and Corynebacterium pilosum" Vet. Microbiol. 13. 75-85 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yanagawa, R: Progress in Veterinary Microbiology and Immunology Causative agents of bovine pyelonephritis Corynebacterium renale, C. pilosum and C. cystitidis. S. Kargar, Basel, 158-174 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ono,E.: Infection and Immunity. 57(3). (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Orino,K.: Infecton and Immunity.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kubota,T.: Jpn.J.Vet.Sci.,. 50. 199-207 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Koga: Jpn.J.Vet.Sci.,. 50. 1277-1278 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 小野恵利子;内貴正治;中沢宗生: 脂質生化学研究. 29. 101-104 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kudo,Y;Yanagawa,R;Hiramura,T: Veterinary Microbiology. 13. 75-85 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kubota,T;Yanagawa,R: Japanese J.of Veterinary Science. 49. 663-672 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ito,H,Ono,E.and Yanagawa,R: Veterinary Microbiology. 14. 165-1710 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Fukuoka,T.;Yanagawa,R: Japanese J.of Veterinary Science. 49. 1073-1079 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Fukuoka,T.;Yanagawa,R: Japanese J.of Veterinary Reseach. 35. 79-86 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 内貴正治: "癌細胞における糖脂質抗原" Oncologia 20巻(No.2)19-32, 14 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 折野宏一: 脂質生化学研究. 28. 157-160 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Orino,K.: Jpn.J.Vet.Res.34. 153 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kudo,Y.: Veterinary Microbiology. 13. 75-85 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Ochanda,J.: J.Biochem.100. 27-33 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Nishimori,K.: Infect.Immun.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Ono,E.: J.Gen.Microb.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 内貴正治: "細胞工学:糖脂質の細胞工学的応用" 秀潤社, 664-669 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yanagawa,R.: "Causative agents of bovine pyelonephritis:Corynebacterium renale,C.pilosum and C.cystitidis in Progress in Veterinary Microbiology and Immunology" S.Kargar,Basel, 158-174 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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