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Basic and practical studies on the immunological prevention of salmonids against bacterial diseases

Research Project

Project/Area Number 61480086
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

IZAWA Hisao  Fac. Vet. Med., Hokkaido Univ., 獣医学部, 教授 (50072351)

Co-Investigator(Kenkyū-buntansha) KODAMA Hiroshi  Fac. Vet. Med., Hokkaido Univ., 獣医学部, 助教授 (20091449)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsSalmonids / Vibrio Anguillarum / Aeromonas Salmonicida / Macrophage / Chemiluminescence / C-Reactive Protein / Activation of Complement / 細菌内毒素 / 防御免疫機構 / Vibrio anguillarum / 魚類のビブリオ病 / エンドトキシン
Research Abstract

Protective immune mechanisms of salmonids against fish pathogen Vibrio anguillarum and Aeromonas salmonicida were investigated.
1. Mechanism of activation of macrophages and the role of the macrophages in protection: Relationship between antibody production in rainbow trout and resistance against bacterial infection was investigated. Though the fish immunized with formalin-killed bacteria resisted the challenge with virulent bacteria, there was no obvious relationships between antibody titer and protection. Living V. anguillarum elicited strong chemiluminescent reaction in peritoneal macrophages of rainbow trout. The activation of macrophages seemed to be related to the resistance of rainbow trout.
2. Production of C-reactive protein (CRP) and the role of CRP in protection: CRP levels in rainbow trout sera increased significantly after bacterial infection. Purified CRP activated complement system of rainbow trout, and suppressed in vitro growth of bacteria. CRP levels were also affected by water temperature.
3. Mechanism of resistance of rainbow trout against bacterial endotoxin: Rainbow trout serum was found to contain higher levels of endotoxin than mammals. Though the amount of endotoxin in serum increased significantly by the injection of endotoxin, no clinical abnormality was observed in fish, indicating that the rainbow trout possessed high resistance to endotoxin.

Report

(3 results)
  • 1988 Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 児玉洋: 獣医学1987. 176-187 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kodama.H.;Honda,M.;Mikami,T.;Izawa,H.: Research in Veterinary Science. 43. 78-84 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kodama,H.;Yamada,F.;Kurosawa,T.;Mikami.T.;Izawa,H.: Journal of Applied Bacteriology. 63. 255-260 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamada,F.;Kodama,H.;Mikami,T.;Izawa,H.: Japanese Journal of Veterinary Science. 50. 1092-1098 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 児玉洋: 獣医学1989.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kodama.H.;Yamada,F.;Murai,T.;Nakanishi,Y.;Mikami,T.;Izawa,H.: Developmental and Comparative Immunology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Murai,T.;Kodama,H.;Naiki,M.;Mikami,T.;Izawa,H.: Developmental and Comparative Immunology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 伊沢久夫、児玉洋ら: "水生動物医学〈各論〉" 日本小動物獣医師会, 248 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 伊沢久夫、児玉洋ら: "獣医伝染病学(第3版)" 近代出版,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi Kodama: "Protective immune mechanisms of fish" Progress in Veterinary Science 1987. 176-187 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi Kodama: "Detection of fish antibody against protein antigen of Aeromonas salmonicida by enzyme-linked immunosorbent assay using biotin-avidin system" Research in Veterinary Science. 43. 78-84 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi Kodama: "Quantitative measurement of endotoxin in rainbow trout (Salmo gairdneri) serum by the chromogenic substrate method" Journal of Applied Bacteriology. 63. 255-260 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fumitaka Yamada: "Chemiluminescence of rainbow trout (Salmo gairdneri) phagocytes and factors affecting their response" Japanese Journal of Veterinary Science. 50. 1092-1098 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi Kodama: "C-reactive protein of fish" Progress in Veterinary Science 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi Kodama: "Activation of rainbow trout (Salmo gairdneri) macrophages and production of C-reactive protein in the early stage of immunization with Vibrio anguillarum" Developmental and Comparative Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takashi Murai: "Isolation and characterization of rainbow trout C-reactive protein" Developmental and Comparative Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yuki Nakanishi: "The activation of rainbow trout (Salmo gairdneri) complement by C-reactive protein"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hisao Izawa: Aquatic Animal Medicine, II. The Japan Small Animal Veterinary Association, 248 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hisao Izawa: Infectious Diseases of Domestic Animals. Kindai Shuppan,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kodama,H.: Journal of Applied Bacterriology. 63. 255-260 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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