| Project/Area Number |
61480120
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Fukuoka University |
|---|
Principal Investigator |
KAMIYA Hiro-o FUKUOKA UNIVERSITY, 薬学部, 教授 (90028366)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Atsushi FUKUOKA UNIVERSITY, 医学部, 助手 (20148878)
TAGAWA Takasuke FUKUOKA UNIVERSITY, 医学部, 教授 (20078736)
HONDA Kenji FUKUOKA UNIVERSITY, 薬学部, 助手 (60140761)
TAKANO Yukio FUKUOKA UNIVERSITY, 薬学部, 助手 (50113246)
|
|---|
| Project Period (FY) |
1986 – 1988
|
| Project Status |
Completed (Fiscal Year 1988)
|
| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥5,500,000 (Direct Cost: ¥5,500,000)
|
|---|
| Keywords | Substance P / Substance K (neurokinin A) / Tachykinins / Receptor subtype / 心循環器調節(血圧調節) / タキキニン・ペプチド / 孤束核 / 神経刺激伝達物質 / 脳動脈 / ペプチド二量体 / サデスタンスKまたはニューロキニンA / タキキニン・ペプチド受容体 / 圧受容器反射 / 化学受容器反射 / 体性ー自律神経反射 / 平滑筋組織 / サブスタンスKまたはニューロキニンA / サブスタンス性ニューロン / サブスタンスP性ニューロン / 中枢神経系 / カプサイシン / 線条体ー黒質系サブスタンスP性ニューロン |
|---|
| Research Abstract |
We studied the physiological characteristics of substance K (SK; neurokinia A) in comparison with those of substance P (SP), because these two tachykinin peptides are derived from a common tachykinin precursor.
(1) The regional localization of SK- and SP-like immunoreactivities (SK-LI and SP-LI) in the brain and peripheral tissues of rats was investigated, and followed by the effects of neonatal treatment of rats with capsaicin on SK-LI and SP-LI. The distribution of SK-LI was similar to that of SP-LI, and ratio of SK to SP was about 1: 1-4. However, the capsaicin treatment of rat caused different changes in the content in various regions of the CNS between SK-LI and SP-LI. (2) Pathways of the striatonigral neuron and of the afferent neuron to the nucleus tractus solitarii (NTS) were demonstrated by a combination of pharmacological and immunochemical studies. These results suggest that SK and SP may be neurotransmitters in these neurons. (3) Neonatal treatment with capsaicin reduced the content of SP-LI in the NTS. Under these conditions, capsaicin-treated rats maintained reflex responses of the renal sympathetic nerve activity to stimulation of baroreceptor. (4) Dimeric analogues of SK and SP COOH-terminal fragments were synthesized and examined the in vivo and in vitro biological activities. Some of dimers showed a highly POtent analogue for a tachykinin receptor subtype.
|
