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Prostaglandins and Platelet-activating Factor in Cultured Hepatocytes - Its Metabolism and Physiological Significance -

Research Project

Project/Area Number 61480125
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionKansai Medical School

Principal Investigator

SAITO Kunihiko  Kansai Medical School, 医学部, 教授 (80077560)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Reiko  Kansai Medical School, 医学部, 助手 (60172483)
SATOUCHI Kiyoshi  Kansai Medical School, 医学部, 講師 (50098125)
OKUMURA Tadayoshi  Kansai Medical School, 医学部, 助教授 (80113140)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordscultured hepatocytes / prostaglandins / receptor / pertussis toxin / 血小板活性化因子 / プロスタグランジンの代謝 / ラット肝細胞 / 初代培養
Research Abstract

I. Prostaglandin(PG) Metabolism in Primary Coultureed Rat Hepatocytes
(1) The hepatocytes could distinguish such active PGs as PGE_1, E_2, D_2 and F_<2<alpha>> from inacive ones, for example, 6-keto-PGF_<1<alpha>> and TXB_2.
(2) The hepatocyte-specific pathway of the active PGs. They were decomposed by a route of <beta>-oxidation to their dinor- and tetranor-compounds, which were quite different from the usual oxidative degradation pathway at C-15 hydroxy residure. The products were excluded to extrahepatic space (BBA, 1986).
(3) The hepatocytes had receptors for PGE and the apparent dissociation constant was 1.2x10^<-8> M (Biochem. Int., 1987).
(4) PGE series inhibited the hoemone-stimulated glycogenolysis of the hepatocytes, but PGD_2, F_<2<alpha>>, dinor- and tetranor-PGE_1 did not (BBA, 1988).
(5) The inhibitory activity was completely abolished when the primary cultured hepatocytes were preincubated with Pertussis toxin (in preparation).
II. Presence of platelet-activating factor (PAF) and Its Inhibitor in Normal Rat Tissues The hepatocytes contained a very minute amount of PAF and other normal rat tissues particularly uterus had a fairy lot of PAF quite independent with inflammatory or immunological stimuli. In the same tissue, the PAF inhibitors were found and identified as a mixture if various molecular species of lysolecithin and sphingomyelin (BBRC, 1986: JBC, 1987).

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Tomoko Sato;Reiko Nakayama;Takayoshi Okumura;Kunihiko Saito: Biochim. Biophys. Acta. 879. 330-338 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Katsuhiko Yasuda;Kiyoshi Satouchi;Kunihiko Saito: Biochim. Biophys. Res. Commun.138. 1231-1236 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kunihiko Saito;Kiyoshi Satouchi;Makoto Oda;Reiko Nakayama: Advances in mass spectrometry 1985, Part B. 1507-1508 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Reiko Nakayama;Katsuhiko Yasukap;Kunihiko Saito: J. Biol. Chem.262. 13174-13179 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tadayoshi Okumura;Tomoko Sago;Kunihiko Saito: Biochim. Biophys. Acta. 958. 179-187 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tadayoshi Okumura;Tomoko Sago;Kunihiko Saito: Submitted for Prostaglandins.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kunihiko Saito;Kiyoshi Satouchi;Minoru Kino;Hirotaro Ogino;Makoto Oda;ed. by A. Kuksis: "GC/MS of molecular species of glycerophospholipids, "Chromatography of Lipids in Biomedical Research and Clinical Diagnosis"" Elsevier, (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tomoko Sago, Reiko Nakayama, Tadayoshi Okumura, Kunihiko Saito: "Metabilism of prostaglandins D_2 and F_<2<alpha>> in primary cultures of rat hepatocytes" Biochim. Biophys. Acta. 879. 330-338 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Katsuhiko Yasuda, Koyoshi Satouchi, Kunihiko Saito: "Platelet-activating factor in normal rat uterus" Biochem. Biophys. Res. Commun.138. 1231-1236 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kunihiko Saito, Kiyoshi Satouchi, Makoto Oda, Reiko Nakayama: "Application of selected ion monitoring to determination of platelet-activating factor" Advances in mass spectrometry 198k, Part B. 1507-1508 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Reiko Nakayama, Katsuhiko Yasuda, Kunihiko Saito: "Existence of endogenous inhibitors of platelet-activating factor(PAF) with PAF in rat uterus" J. Biol. Chem.262. 13174-13179 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tadayoshi Okumura, Yomoko Sago, Kunihiko Saito: "Effect of prostaglandins and their analogues on hormone-stimulated blycogenolysis in primary cultures of rat hepatocytes" Biochim. Biophys. Acta. 958. 179-187 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kunihiko Saito, Kiyoshi Satouchi, Minoru Kino, Hirotaro Ogino, Makoto Oda: ed. by A. Kuksis, Elsevier. GC/MS of molecular species of glycerophospholipids, "Chromatography of Lipids in Biomedical Research and Clinical Diagnosis", (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tomoko Sago: Biochim.Biophys.Acta. 879. 330-338 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tadayoshi Okumura: Biochem.Int.14. 443-449 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Katsuhiko Yasuda: Biochem.Biophys.Res.Commun.138. 1231-1236 (1983)

    • Related Report
      1986 Annual Research Report
  • [Publications] Makoto Oda: J.Biochem.100. 1117-1123 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kunihiko Saito: Adv.Mass Spectrometry 1985,Part B. 1507-1508 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kunihiko Saito: 23rd International Symposium,Advances in Chromatography,abstracts. 37-38 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Kunihiko Saito: "GC/MS of molecular species of glycerophospoliPids in"Chromatography of Lipids in Biomedical Research"ed.A.Kuksis" Elsevier Science Publishers, (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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