| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Research Abstract |
I. Prostaglandin(PG) Metabolism in Primary Coultureed Rat Hepatocytes
(1) The hepatocytes could distinguish such active PGs as PGE_1, E_2, D_2 and F_<2<alpha>> from inacive ones, for example, 6-keto-PGF_<1<alpha>> and TXB_2.
(2) The hepatocyte-specific pathway of the active PGs. They were decomposed by a route of <beta>-oxidation to their dinor- and tetranor-compounds, which were quite different from the usual oxidative degradation pathway at C-15 hydroxy residure. The products were excluded to extrahepatic space (BBA, 1986).
(3) The hepatocytes had receptors for PGE and the apparent dissociation constant was 1.2x10^<-8> M (Biochem. Int., 1987).
(4) PGE series inhibited the hoemone-stimulated glycogenolysis of the hepatocytes, but PGD_2, F_<2<alpha>>, dinor- and tetranor-PGE_1 did not (BBA, 1988).
(5) The inhibitory activity was completely abolished when the primary cultured hepatocytes were preincubated with Pertussis toxin (in preparation).
II. Presence of platelet-activating factor (PAF) and Its Inhibitor in Normal Rat Tissues The hepatocytes contained a very minute amount of PAF and other normal rat tissues particularly uterus had a fairy lot of PAF quite independent with inflammatory or immunological stimuli. In the same tissue, the PAF inhibitors were found and identified as a mixture if various molecular species of lysolecithin and sphingomyelin (BBRC, 1986: JBC, 1987).
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