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Structure and the regulation of the gene expression of the human insulin receptor gene and its abnormalities

Research Project

Project/Area Number 61480131
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionTokushima Univ. (1988)
Kumamoto University (1986-1987)

Principal Investigator

EBINA Yousuke  Tokushima Univ. Professor, 酵素科学研究センター, 教授 (00112227)

Co-Investigator(Kenkyū-buntansha) TAKIGUCHI Masaki  Kumamoto Univ. Assistant Professor, 医学部, 講師 (40179578)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsHuman insulin receptor gene / Regulation of the gene expression / 糖尿病 / プロモーター / インスリン受容体 / チロシンキナーゼ / インスリン作用機構 / インスリン受容体遺伝子 / site directed mutagenesis
Research Abstract

1. Analysis of the structure-function relationship of the human insulin receptor and its abnormality in Diabetes mellitus. We have already reported that the primary structure of the human insulin receptor and that the tyrosine kinase of the subunit of its receptor is essential for insulin signal transduction. We new report a patient with insulin resistant diabetes who has a deletion of a large part of the tyrosine kinase domain in one allele of the insulin receptor gene. This syndrome was transmitted from mother to daughter, with apparently autosomal dominant trait.
2. Regulation of the gene expression of the human insulin receptor gene. We have already reported that the promoter structure of the human insulin receptor gene and that the four G-C boxes which are located at -600 bp stimulate the transcription 10 times. We have identified the binding of the Sp1 transcription activating factor to these G-C boxes.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Ebina,Y.;et al.: Proc.Natl.Acad.Sci.USA. 84. 704-708 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Araki,E,;Ebina,Y.;et al.: J.Biol.Chem.262. 16186-16191 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Araki,E.;Ebina,Y.;et al.: Nucleic Acids Res.16. 1627 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Taira,M.;Ebina,Y.;et al.: Nature.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ebina Y. et al.: "Replacement of lysine residue 1030 in the putative ATP binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity" Proc. Natl. Acad. Sci. USA. 84. 704-708 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Araki E., Ebina Y. et al.: "Characterization of the promoter region of the human insulin receptor gene" J. Biol. Chem.262. 16186-16191 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Araki E., Ebina Y. et al.: "pSVOOCAT: low background CAT plasmid" Nucleic Acids Res.16. 1627 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Taira M., Ebina Y. et al.: "Insulin resistant diabetes due to a deletion of tyrosine kinase domain of the insulin receptor gene" Nature.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ebina,Y.;et al.: Proc.Natl.Acad.Sci.USA. 84. 704-708 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Araki,E.;Ebina,Y.;et al.: J.Biol.Chem.262. 16186-16191 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Araki,E.;Ebina,Y.;et al.: Nucleic Acids.Res.16. 1627 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Taira,M.;Ebina,Y.;et al.: Nature.

    • Related Report
      1988 Annual Research Report
  • [Publications] Eiichi Araki: The Journal of Biological Chemistry. 262. 16186-16191 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 蛯名洋介: 現代化学. 12. 61-68 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 蛯名洋介: 代謝. 24. 33-40 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yasuyoshi Nishida: Biochemical and Biophysical Research Communications. 141. 474-481 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yousuke Ebina: Proc.Natl.Acad.Sci.USA.

    • Related Report
      1986 Annual Research Report
  • [Publications] 蛯名洋介: 生化学. 58. 477-482 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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