| Project/Area Number |
61480141
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Institue of Tropical Medicine, Nagasaki University (1987-1988)
国立予防衛生研究所 (1986) |
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Principal Investigator |
WADA Yoshito Professor, Dept of Med. Entomol., Inst. Trop. Med., Nagasaki Univ., 病害動物学部門, 教授 (60039493)
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| Co-Investigator(Kenkyū-buntansha) |
SHONO Toshio Laboratory Chief, Dept of Med. Entomol., National Inst. Health, 衛生昆虫部, 殺虫剤室長 (80011922)
TOMITA Takashi Investigator, Dept of Med. Entomol., National Inst. Health, 衛生昆虫部, 研究員 (20180169)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Housefly / Insecticide resistance / Pyrethroids / kdr gene / 性決定機構 |
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| Research Abstract |
With a background that abnormal sex determination in the housefly (Musca domestica) became known in the 1960's and recently increased, 19 populations in Japan were examined by crossing method. the results revealed that abnormal mechanism is common and widespread, by crossing method. The results revealed that abnormal mechanism is common and widespread, in which male determining factor M on autosomes and female determining factor F epistatic to M factor(s) are involved. Two housefly populations in Yumenoshima (Tokyo) and Haga (Tochigi) were examined in 1986 for insecticide susceptibility and found to have high resistance to organophosphorus insecticides, and in addition, to pyrethroids in Haga population. The kdr gene responisible for pyrethroid resistance was found in the frequency of 0.2 - 1.0 in 10 populations in Gunma and Tochigi prefectures. it is the newly found fact that the kdr gene is widely distributed in high frequencies in Japan. The kdr gene of houseflies was monitored for
… More
two years from October 1986 in two animal houses in Haga. The increase in frequency was clearly associated with use of pyrethroid insecticides and the decrease seemed to be due to the migration of susceptible houseflies from the surroundings. The frequency of the kdr gene in the laboratory did not change up to the sixth generation of houseflies kept without selection by insecticide, but the evidence for the reduction in the fitness of houseflies with kdr could not be obtained. The linkage disequilibrium was not detected in two populations of Gunma and Tochigi prefectures between an abnormal sex determining factor m and a pyrethroid resistance gene kdr. Methods for delaying the development of insecticide resistance in housefly populations were theoretically considered. It was shown that when the frequency of a resitant gene is low, a high dosage of an insecticide should be applied so as to kill nearly all heterozygotes and a proportion of houseflies are allowed to escape from the insecticide by mosaic spray or rotation of two or more insecticides with different resistance mechanism. Less
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