| Project/Area Number |
61480142
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | Gunma University |
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Principal Investigator |
HASHIMOTO Hajime Gunma University, 医学部, 教授 (90008235)
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| Co-Investigator(Kenkyū-buntansha) |
IKE Yasuyoshi Gunma University, 医学部, 講師 (60125820)
IYOBE Shizuko Gunma University, 医学部, 助教授 (90008318)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | Pseudomonas aeruginosa / Gentamicin resistance / New quinolone-resistance / Conjugative plasmid / Nonconjugative plasmid / Chromosomal resistance / 外膜タンパク / 伝達性プラスシド / 染色体性耐性 / 血清型 / ファージ型 / セラチア / 腸球菌 / 耐性獲得 / 外膜透過性 / ノルフロキサシン / フェロモン / Tn2001 |
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| Research Abstract |
We examined 385 clinical isolates of P.aeruginosa and selected 90 strains resistant to at least one of 23 antibacterials tested. Using these strains, we determined their serum type, phage type and plasmid type and analysed their relationship with their drug-resistances. There found greatest differences between drug-resistant and sensitive strains in the case of gentamicin. GM-resistant strains belonged mostly to serum type I or nontypable. All of the GM-resistant isolates from different origin isolated in 1984 expressed the same multiple resistance, were grouped to serum type I and phage nontypable, and possessed nonconjugative miniplasmid encoding the multiple resistance. The same type strains have been isolated sporadically since 1985. Conjugally transmissible GM-resistant isolates showing different multiple resistance were found since 1985 and most of them were serum-nontypable. Thus we suspect the nosocomial infection in our hospital.
Developement of drug-resistance owing to the abundant use of antimicrobials was demonstrated in the increase of norfloxacin-resistant P.aeruginosa accompanied with the increased use of new quinolone antimicrobials. The drug-sensitive clinical isolates were able to mutate to higher level of quinolone resistance with or without accompanying the change of sensitivities to other drugs. Mutants expressing the change of sensitivity to 2 or more kind of antimicrobials showed various changes in their outer-membrane proteins, resulting in the change of drug-permeability.
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