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Epidemiological and genetical analyses of clinical isolates resistant to newly developed antimicrobials

Research Project

Project/Area Number 61480142
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionGunma University

Principal Investigator

HASHIMOTO Hajime  Gunma University, 医学部, 教授 (90008235)

Co-Investigator(Kenkyū-buntansha) IKE Yasuyoshi  Gunma University, 医学部, 講師 (60125820)
IYOBE Shizuko  Gunma University, 医学部, 助教授 (90008318)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥3,300,000 (Direct Cost: ¥3,300,000)
KeywordsPseudomonas aeruginosa / Gentamicin resistance / New quinolone-resistance / Conjugative plasmid / Nonconjugative plasmid / Chromosomal resistance / 外膜タンパク / 伝達性プラスシド / 染色体性耐性 / 血清型 / ファージ型 / セラチア / 腸球菌 / 耐性獲得 / 外膜透過性 / ノルフロキサシン / フェロモン / Tn2001
Research Abstract

We examined 385 clinical isolates of P.aeruginosa and selected 90 strains resistant to at least one of 23 antibacterials tested. Using these strains, we determined their serum type, phage type and plasmid type and analysed their relationship with their drug-resistances. There found greatest differences between drug-resistant and sensitive strains in the case of gentamicin. GM-resistant strains belonged mostly to serum type I or nontypable. All of the GM-resistant isolates from different origin isolated in 1984 expressed the same multiple resistance, were grouped to serum type I and phage nontypable, and possessed nonconjugative miniplasmid encoding the multiple resistance. The same type strains have been isolated sporadically since 1985. Conjugally transmissible GM-resistant isolates showing different multiple resistance were found since 1985 and most of them were serum-nontypable. Thus we suspect the nosocomial infection in our hospital.
Developement of drug-resistance owing to the abundant use of antimicrobials was demonstrated in the increase of norfloxacin-resistant P.aeruginosa accompanied with the increased use of new quinolone antimicrobials. The drug-sensitive clinical isolates were able to mutate to higher level of quinolone resistance with or without accompanying the change of sensitivities to other drugs. Mutants expressing the change of sensitivity to 2 or more kind of antimicrobials showed various changes in their outer-membrane proteins, resulting in the change of drug-permeability.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 塚田勝彦,伊豫部志津子: 北関東医学. 38. 157-163 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 塚田勝彦,伊豫部志津子 他: 北関東医学. 38. 269-274 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 加藤広行,伊豫部志津子 他: 感染症学雑誌. 62. 938-943 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Ike,;H.Hashimoto. 他: J.Clin.Microbiol. 25. 1524-1528 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 橋本一: "新キノロン剤の臨床" ライフサイエンス, 15-26 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Katsuhiko Tsukada: "Bacteriological studies on Pseudomonas aeruginosa isolated from patients in Gunma University Hospital" The Kitakanto Medical Journal. 38. 157-163 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Katsuhiko Tsukada: "Relationship between the drug-resistance and the serotype of Pseudomonas aeruginosa isolated from patients in Gunma University Hospital" The Kitakanto Medical Journal. 38. 269-274 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroyuki Katoh: "The phage typing of Pseudomonas aeruginosa isolated from patients in Gunma University Hospital" The Journal of the Japanese Association for Infectious diseases. 62. 938-943 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yasuyoshi Ike: "High incidence of hemolysin production by E.faecalis strains associated with human parenteral infections" J. Clin. Microbiol.25. 1524-1528 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hajime Hashimoto: Clinical application of new quinolones. Life Science, 15-26 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 塚田勝彦,伊豫部志津子 他: 北関東医学. 38. 157-163 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 塚田勝彦,伊豫部志津子 他: 北関東医学. 38. 269-274 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 加藤広行,伊豫部志津子 他: 感染症学雑誌. 62. 938-943 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.IKe,H.Hashimoto 他: J.Clin.Microbiol.25. 1524-1528 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 橋本一: "新キノロン剤の臨床" ライフサイエンス, 15-26 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 加藤広行;伊予部志津子;橋本一他: 日本感染症学雑誌.

    • Related Report
      1987 Annual Research Report
  • [Publications] Silvia.Trati;伊予部志津子;橋本一他: J.Clin.Microbiol.

    • Related Report
      1987 Annual Research Report
  • [Publications] 代居敬子;井上松久;橋本一: J.Antimicrob.Chemother.

    • Related Report
      1987 Annual Research Report
  • [Publications] 代居敬子;井上松久;橋本一: Antimicrob.Ag.Chemother.

    • Related Report
      1987 Annual Research Report
  • [Publications] 池康嘉: 日本細菌学雑誌. 41. 497-511 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Hirai: Antimicrobial Agents and Chemotherapy. 29. 535-538 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Hirai: Antimicrobial Agents and Chemotherapy. 30. 248-253 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 田中徳滿,橋本一: "赤痢菌の薬剤耐性とRプラスミド/日本の感染性腸炎" 菜根出版, 17 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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