| Project/Area Number |
61480146
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | Shouwa University School of Dentistry (1987)
Tokyo Medical and Dental University (1986) |
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Principal Investigator |
GOTO Nobuichi Showa Universiy Shool of Dentistry, Professor, 歯学部, 教授 (10077175)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Transposon / Transposition immunity / <gamma><delta> sequence / Synthetic DNA / DNA塩基配列 |
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| Research Abstract |
A plasmid containing <gamma><delta> cannot acquire another copy of <gamma> <delta>by transposition (transposition immunity).We previously found that the 38-bp sequence of the <delta> terminal is sufficient to mediate the immunity. In the present study. however, the <gamma>-terminal 38 bp was shown to have weaker (1/45) immunity activity than the other. The <gamma>-terminal 38-bp sequence was then synthesized with and without a flanking 5-bp sequence and cloned into the SmaI-site of the plasmid pUC13 in order to see the effect of the flanking base sequence on the efficiency of transposition immunity. When the<gamma>-terminal 38 bp was cloned together with one of the natural flanking sequences GTAA. in stead of the artificial SmaI-site sequence TCCCC, immunity activity increased by 7-fold. It seems to suggest that the specificity of base sequence required for transposition immunity prefers AT-rich flanking sequence to GT-rich one. As for the base sequence between 36th and 38th, the substitution of TAT with ATG reduced the immunity by 1/28. Since the sequence ATG differs only one base from the sequnce AAG (the <delta>-terminal suquence), which had the strongest immunity, the specificity fo the base 37 must be extremely high.
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