• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

New drug-dilivery system for ganglionic viral infections.

Research Project

Project/Area Number 61480150
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

YUZO Iwasaki  Professor, Dept. of Neurological Sciences, School of Medicine, 医学部, 教授 (00142927)

Co-Investigator(Kenkyū-buntansha) 今野 秀彦  東北大学, 医学部, 助手 (10091688)
YAMAMOTO T.  Associate Professor, i.b.d. Dept. of Neurological Sciences, School of Medicine, 医学部, 助教授 (10106487)
KONNO H.  Assistant, i.b.d. Dept. of Neurological Sciences, School of Medicine (KAWAZOE,Yosh)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsHerpes simplex virus / persistent virus infection / rabies / 狂犬病 / 三叉神経節 / 免疫組織化学 / in situ hybridization / 逆行性軸索法
Research Abstract

For the study of eradication herpes simplex virus (HSV) persistence in the ganglion, A new rat model was established using the trigeminal ganglion and mental nerve. After intraneural injection of 2-3 micron of F strain of HSV, persistent infection was established in more than 80% of rats where viral DNA was detactable by in situ hybridization with biotinilated cDNA up to 502 after virus inoculation although no viral antigens were detectable 5 day after virus inoculation. A single injection of doxorubicin into the mental nerve reduced the incidence of virus isolation from cultured ganglion tissue from 31/37 (control) to 3/37. Therefore, retrograde axoplasmic transport of appropriate drugs can be used for the treatment of persistent virus infection in the ganglionic cells. Potential use of retrograde axoplasmic flow for acute infection of the CNS after neural spread of virus was explored in experimental rabies in rats.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Iwasaki, Y. et al.: Journal of Virology. 59. 242-248 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Iwasaki, Y et al.: Acta Neuropathologica(Berlin). 70. 79-81 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hayashi. K.;Iwasaki, Y.;Yanagi, I: Journal of Virology. 57. 942-951 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岩崎裕三: 神経疾患の進歩. 30. 996-1002 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岩崎裕三, 山本裕高: 薬局. 38. 709-713 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 岩崎裕三: 医学のあゆみ. 142. 557-559 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 伊藤正雄, 桑原武男編, 岩崎裕三: "脳の科学:ウイスルと神経疾患" 同文書院, 571-585 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Iwasaki, Y.;ed. G. Bear: ""Natural History of Rabies" Spred of virus within the central nervous system" CRC Press, U. S. A., (198-)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Iwasaki,Y.et al.: Journal of Virology. 59. 242-248 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Iwasaki,Y.et al.: Acta Neuropathologica(Berlin). 70. 79-81 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Hayashi,K.;Iwasaki,Y.;Yanagi,K.: Journal of Virology. 57. 942-951 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岩崎裕三: 神経研究の進歩. 30. 996-1002 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 岩崎祐三,伊藤正男,桑原武夫.編: "脳の科学 ウイルスと神経疾患" 同文書院, (1987)

    • Related Report
      1986 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi