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Analysis of adaptive expression of a T cell self-recognizing structure in radiation bone marrow chimeras.

Research Project

Project/Area Number 61480155
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionUniversity of Tokyo

Principal Investigator

ASANO Yoshihiro  University of Tokyo, Associate Professor, 医学部, 助教授 (70114353)

Co-Investigator(Kenkyū-buntansha) TADA Tomio  University of Tokyo, Professor, 医学部, 教授 (10009136)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsMajor histocompatibility complex (MHC) / I-J / T cell receptor (TcR) / Ca^<++> / Influx / 主要組織適合遺伝子複合体(MHC) / I-J / T細胞抗原レセプター / サプレッサーT細胞 / ヘルパーT細胞 / T細胞クロン / MHC拘束 / 放射線照射骨髄キメラ / T細胞認識
Research Abstract

Monoclonal antibodies (mAb) against I-region-controlled epitopes expressed exclusively on mature functional T cells but not on B cells and macrophages (anti-Iat and anti-I-J) were found to inhibit class II MHC-restricted cell interactions in the immunological circuit. The present study was undertaken to analyze a mechanism of expression of Iat and I-J epitopes on T cells and to study a functional role of these molecules in immune regulation. Four essential findings were obtained: first, Iat/I-J epitopes undergo systematic alterations according to the environmental MHC in radiation bone marrow chimeras. Secondary, the I-J epitope is not an idiotypic determinant of T cell antigen-receptor (TcR) but is on a distinct molecule from TcR. Third, we have been able to identify the I-J molecule of cloned T cells by the specific immunoprecipitation. The I-J molecule detected is a 90K dimeric molecule composed of 45K glycopeptide subunits distinct from TcR or CD28. Finally, the cross-linking of I-J molecules with specific mAb resulted in the inhibition of Ca_<++> influx induced via the specific stimulation by antigen and MHC. These results indicated that I-J (probably Iat as well) is a T cell surface molecule that transduces a signal to regulate the hydrolysis of intracellular messengers of T cell activation induced via the TcR/T3 complex.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report

Research Products

(72 results)

All Other

All Publications

  • [Publications] Tada,Tomio: BioEssays. 4. 283-285 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: Journal of Molecular and Cellular Immunology. 2. 232-233 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Immunology. 138. 667-673 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Abe,Ryo: Journal of Immunology. 138. 2964-2969 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Experimental Medicine. 165. 87-96 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Iwashima,Makio: International Archives of Allergy and Applied Immunology. 83. 129-137 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koyasu,Shigeo: J.Biol.Chem.262. 4689-4695 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sano,Kunio: Journal of Experimental Medicine. 165. 1284-1295 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuji,Moriya: European Journal of Immunology. 17. 675-681 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama,Toshinori: Journal of Immunology. 139. 3935-3941 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Experimental Medicine. 166. 1613-1626 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Immunology. 140. 419-426 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama,Toshinori: European Journal of Immunology. 18. 761-765 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Immunology. 142. 365-372 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama,Toshinori: Immunology. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama,Toshinori: International Immunology. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Idiotypes" Academic Press, 249-262 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Progress in Immunology V" Academic Press, 341-347 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "New Horizons in Animal Models for Autoimmune Disease" Academic Press, 341-347 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Hー2 Antigens" Plenum Press Corp, 435-440 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Cellular Basis of Immune Modulation." Alan R.Liss,Co., 341-351 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada,Tomio: "Proceedings of the 19th International Symposium of the Princess Takamatsu Cancer Research Fund" (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: "Somatic generation of a genetic polymorphism: Towards the solution of the I-J enigma." BioEssays. 4. 283-285 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: "The origin of MHC-restriction of suppressor T cells. A commentary." Journal of Molecular and Cellular Immunology. 2. 232-233 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano, Yoshihiro: "Analysis of two distinct B cell activation pathways mediated by a monoclonal T helper cell. I. MHC-restricted activation of B cells by an IL-2 dependent pathway." Journal of Immunology. 138. 667-673 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Abe, Ryo: "Epitopes associated with the MHC restriction site of T cells. I. Selecrive expression of Iat epitopes on H-2-restricted helper T cells." Journal of Immunology. 138. 2964-2969 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano, Yoshihiro: "Epitopes associated with the MHC restriction site of T cells. II. Somatic generation of Iat epitopes on T cells in radiation bone marrow chimeras." Jounral of Experimental Medicine. 165. 87-96 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Iwashima, Makio: "Alterations in the proliferative responses of T cells from aged and chimeric mice." International Archives of Allergy and Applied Immunology. 83. 129-137 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koyasu, Shigeo: "Signals for activation and proliferation of murine T lymphocyte clones." J. Biol. Chem.262. 4689-4695 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sano, Kunio: "Major histocompatibility complex(MHC)-restricted minimal regulatory circuit initiated by a class II-autoreactive T cell clone." Journal of Experimental Medicine. 165. 1284-1295 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsuji, Moriya: "Shared idiotopes between anti-class II monoclonal antibodies and major histocompatibility complex (MHC) recognition site of T cells." European Journal of Immunology. 17. 675-681 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama, Toshinori: "Major histocompatibility complex-restricted augmenting T cells induced by in vitro cultivation of antigen-primed T cells. A new parallelism between suppressor and augmenting circuits." Journal of Immunology. 139. 3935-3941 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano, Yoshihiro: "Epitopes associated with MHC restriction site of T cells. III. I-J epitopes on MHC-restricted T helper cells." Journal of Experimental Medicine. 166. 1613-1626 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano, Yoshihiro: "Analysis of two distinct B cell activation pathways mediated by a monoclonal T helper cell. II. T helper cell secretion of interleukin 4 selectively inhibits antigen-specific B cell activation of cognate, but not noncognate, interaction with T cells." Journal of Immunology. 140. 419-426 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama, Toshinori: "Epitopes associated with MHC restriction site of T cells. IV. I-J epitopes on MHC-restricted cloned T cells." European Journal of Immunology. 18. 761-765 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano, Yoshihiro: "Generation of T cell repertoire: Two distinct mechanisms for generation of T suppressor cells, T helper cells, and T augmenting cells." Journal of Immunology. 142. 365-372 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama, Toshinori: "I-J and mechanism of immunosuppression." Immunology. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakayama, Toshinori: "Biochemical identification of I-J as a novel dimeric surface molecule on mouse helper and suppressor T cell clones." International Immunology. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Utsunomiya, Naoko: "Unidirectional inhibition of early signal transduction of helper T cells by cloned suppressor T cells."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Furutani, Makoto: "Coexpression of two T-cell receptor -chain alleles in a single functional T cell clone."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: Idiotypes. Academic Press (New York), 249-262 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: Progress in Immunology V. Academic Press (New York), 341-347 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: H-2 Antigens.Plenum Press Corp., 435-440 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tada, Tomio: Cellular Basis of Immune Modulation.Alan R. Liss, Co., 341-351 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Asano,Yoshihiro: Journal of Immunology. 140. 419-426 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakayama,Toshinori: European Journal of Immunology. 18. 761-765 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Asano,Yoshihiro: Journal of Immunology. 142. 365-372 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakayama,Toshinori: Immunlogy. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakayama,Toshinori: International Immumology. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Utsunomiya,Naoko: Submitted.

    • Related Report
      1988 Annual Research Report
  • [Publications] Furutani,Makoto: Submitted.

    • Related Report
      1988 Annual Research Report
  • [Publications] 浅野喜博: 臨床免疫. 20. 221-228 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 浅野喜博: 感染・炎症・免疫. 18. 263-275 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 浅野喜博: "現代免疫学" 医学書院, 57-67 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tada,Tomio: "Cellular Basis of Immune Modulation." Alan R.Liss,Co., 341-351 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 浅野喜博: "Annual Review 免疫1988" 中外医学社, 68-78 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tada,Tomio: "1989" Proceedings of the 19th International Symposium of the Phincess Takamatsu Cancer Research Fund,

    • Related Report
      1988 Annual Research Report
  • [Publications] Asano.Y: Journal of Immunology. 138. 667-673 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nakayama.T: Journal of Immunology. 139. 3935-3941 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Abe.R: Journal of Immunology. 138. 2964-2969 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Asano.Y: Journal of Experimental Medicine. 165. 87-96 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Asano.Y: Journal of Experimental medicine. 166. 1613-1626 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Iwashima.M: International Archives of Allergy and Applied Immunology. 83. 129-137 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sano.K: Journal of Experimental Medicine. 165. 1284-1295 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Tsuji.M: European Journal of Immunology. 17. 675-681 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Asano,Y.: J.Exp.Med.165. 87-96 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Sano,K.: J.Exp.Med.165(5). (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Asano,Y.: J.Immunol.138. 667-673 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Abe,R.: J.Immunol.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tsuji,M.: Eur.J.Immunol.17. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Koyasu,S.: J.Biol.Chem.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tada,T.: "Idiotypes.(The immune circuit:Somatic generation of epitopes involved in the mutual recognition by different subsets of T cells)" Academic Press, -262)408 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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