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Analysis of regulatory mechanism for autoantibody production by using human T and B cell clones.

Research Project

Project/Area Number 61480177
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

SASAKI Takeshi  Instructor, Tohoku University School of Medicine, 医学部付属病院, 講師 (50110656)

Co-Investigator(Kenkyū-buntansha) ENDO Kazuyasu  Instructor, Tohoku University School of Medicine, 医学部付属病院, 講師 (60125507)
SUGAMURA Kazuo  Professor, Tohoku University School of Medicine, 医学部, 教授 (20117360)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordsanti-DNA antibody / B cell clone / T cell clone / autoantibody / 全身性エリテユトーデス / B細胞クローン / T細胞クローン / 全身生エリテマトーデヌ / SLE(全身生エリテマトーデス) / イディオタイプ / クローン / 全身性エリテマトーデス
Research Abstract

1. We obtained six human B cell clones producing monoclonal anti-DNA antibodies by clonal transformation methods. anti-DNA idiotypes expressed on these surface of the clones were widely ditributed among human B cell clones. Anti-idiotypic antibody producing clones were also detected in patients with inactive SLE as well as in healthy subjects, indicating the idiotypic regulation of anti-DNA autoantibody production.
2. We established T cell clones expressing anti-DNA idiotypes by using clonaltrasnformation methods, where SLE-drived T cells were cocultured with HTLV-1-infected T cell clones(MT-1 or KAN). The obtained T cell clones, however, failed to have ability to regulate anti-DNA antibody production in vitro. The studies are in progress for obtaining T cell clones responsible for autoantibody production.
3.Anti-idiotypic antibodies to numan anti-DNA antibodies had ability to regulate anti-DNA antibody production in human in vitro. We extended the application of regulatory ability of anti-idiotypic antibody to the therapy of autoimmune diseases in autoimmune mice in vivo. The administration of anti-idiotypic antibodies conjugated with neocarzino-statin brought into the elongation on the survival and the deterioration of development of lupus nephritis in the treated mice.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report

Research Products

(29 results)

All Other

All Publications

  • [Publications] T.Sasaki;E.Tamate,et al.: Journal of Immunology. 142. 1159-1165 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.Sano;T.Sasaki,et al.: Scandinavian Journal of Immunolgy. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Sasaki;Y.Koide;K.yoshinaga: Methods in Enzymoiogy. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Itoi;T.Sasaki,et al.: American Journal of Gastrnal of Gastroenterology. 84. 187-191 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Sasaki,et al.: Journal of Rheumatology. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Sasaki,et al.: Journal of Clinical Investigation. 82. 748-754 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Sasaki;E.Tamate et al.: "In vitro manipulation of human anti-DNA antibody production by antiidiotypic antibodies conjugated with neocarzinostatin." Journal of Immunology. 142. 1159-1165 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.Sana; T.Sasaki et al.: "Binding properties of human anti-DNA antibodies to cloned DNA fragments." Scandinavian Journal of Immunology.(1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Sasaki;Y.Koide; K.Yoshinaga: "Immune suppression of anti-DNA antibody production using anti-idiotypic antibo-neocarzinostatin-conjugates." Methods and Enzymology. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Sasaki et al.: "Binding specificity of anti-idiotypic autoantibodies to anti-DNA antibodies in humans." Journal of Clinical Investigation. 82. 748-754 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] O.Takai; T.Sasaki; et al.: "Epstein-Barr virus-transformed B cells bearing idiotypes of anti-DNA autoantibodies." Journal of Clinical Immunology.8. 193-199 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Sasaki;E.Tamate;et al: Journal of Immunology. 142. 1159-1165 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] H.San;T.Sasaki;et al: Scandinavian Jounal of Immunology. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Sasaki;Y.Koide;K.Yoshinaga.: Methods in Enzymology. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Itoi;T.Sasaki;et al.: American Journal Gastroenterology. 84. 187-191 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.sasaki;et al.: Journsl of Rheumatology. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.sasaki;et al.: Journal of Clinical Investigation. 82. 748-754 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kitagawa,Y.;T.Sasaki: J.Immunol.Methods. 96. 7-10 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Muryoi,T.;Sasaki,T.;et al: Tohoku J.Exp.Med.152. 253-258 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Muryoi,T.;Sasaki,T.;et al: Clin.Exo.Immunol.71. 67-72 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Takai,O;Sasaki,T.: J.Clin.Immunol.8. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sasaki,T.;et al: J.Clin.Invest.(1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 佐々木毅: Med.Immunol.13. 737-741 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yoshichika Kitagawa;Takeshi Sasaki: Journal of immunological Mehods. 96. 7-10 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Takeshi Sasaki;Tai Muryoi et.al.: Journal of Clinical Investigation. 77. 1382-1386 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 佐々木毅: 最新医学. 41. 1979-1986 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 佐々木毅: 臨床免疫. 18. 202-210 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 関口幸雄,佐々木毅: 臨床医. 12. 634-636 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 佐々木毅: 臨床医. 12. 662-663 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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