| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI OSAMU Juntendo Univ.,School of Med.,Dept. of Med.,Assist. Prof., 医学部・内科, 助手 (80163624)
HIRANO TAKAO Juntendo Univ.,School of Med.,Dept. of Med.,Assist. Prof., 医学部・内科, 講師 (10165186)
TAKASAKI YOSHINARI Juntendo Univ.,School of Med.,Dept. of Med.,Assist. Prof., 医学部・内科, 講師 (80154772)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
Circulating immunecomplexes (CIC) have been detected among the patients with autoimmune diseases such as Systemic lupus erythematosus (SLE) and Rheumatoid arthritis(RA). In spite of the development of the assay system, etiology of organ involvemeent has not been fully characterized yet. Utilizing novel CIC detection assay with murine menoclonal anti-human C3dg antibody and monoclonal anti-human IgG Fc antibody (AC3dg), we havve tried to investigate the characterization of CIC and to study the effect of CIC for organ involvements. The assay (AC3dg) was sensitive and specific and has not been suffered by Rheumatoid factors in vivo and also in vitro. CIC level in each disease is as fllows; in SLE AC3dg 5.49+5.68ug/ml, Clg solidphase assay(ClqS) 3.74+4.00 ug/ml, in RA, AC3dg 5.56+4.51ug/ml,ClqS 2.43+1.96ug/ml, in mixed connective tissuee disease AC3dg 6.02+3.68ug/ml, ClqS 2.92+3.42ug/ml, in Progressive systemic sclerosis AC3dg 4.25+1.31ug/ml, ClqS 2.77+1.87ug/ml,in polyarteritis nodosa AC3dg 3.90+2.74ug/ml, ClqS 1.90+0.14ug/ml, and in Behchet's disease, AC3dg 4.98+2.26ug/ml, ClqS 2.38+1.72ug/ml. In RA patients, double filtration plasmapheresis decreased the CIC concentration from 6.01+3.62ug/ml down to 4.10+2.41ug/ml (decreace rate of 33%). And in SLE patients, low complementemia group showed higher CIC levels (AC3dg 7.62+11.81ug/ml, ClqS 4.17+2.91ug/ml) than normocomplementemia group (AC3dg 5.49+5.83ug/ml, ClqS 2.58+1.94ug/ml). On the other hand interestingly, patients with proteinuria showed lower CIC level(AC3dg 4.14+2.86ug/ml,ClqS 2.58+1.94ug/ml) than without proteinuria (AC3dg 5.76+6.09ug/ml, ClqS 3.84+4.24ug/ml). These data including the result in which AC3dg allows us to detect the antigen specific immunecomplexes formed in vitro such as CIC including PCNA (proliferating cell nuclear antigen), suggested that this may be te useful tool to study the etiology of the organ involvementand to follow up the patients.
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