| Project/Area Number |
61480187
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | University of Tokyo |
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Principal Investigator |
TODA Gotaro Associate Professor Faculty of Medicine, University of Tokyo, 医学部(病), 助教授 (40090500)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Yoshihiro Medical Staff University of Tokyo Hospital, 医学部, 医員 (80177063)
IKEDA Yusei Assistant Professor Health Service Center, Univeristy of Tokyo, 保健センター, 助手 (80133073)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1987: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1986: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | Calmodulin / Cytoskeleton / Liver plasma membrane / Cytoskeleton-related proteins / Chronic hepatitis / Acute hepatitis / Liver cirrhosis / Calmodulin antibody / 自己抗体 / 肝疾患 / 【Ca^π】結合タン白 / 自己免疫性肝炎 |
|---|
| Research Abstract |
In this study, we found the autoantibody to calmodulin (CaM) which had never been described, and concentrated on clarification of clinical and pathophysiological significance of this autoantibody. The autoantibody to CaM (CaM antibody) was assayed by enzyme-linked immunosorbent assay (ELISA). Immunoglobulin bound to bovine brain CaM fixed to immunoplate was determined using HRP-conjugated antihuman IgG, IgM ad IgA. CaM antibody was decreased by absorption of serum with bovine brain CaM or human erythrocyte CaM, not with actin, tropnin C or DNA. The presence of CaM antiobody was also confirmed by immunoblotting method. IgG class antibody was found in the sera from the patients with collagen diseases as well as those with chronic liver diseases, whereas IgM class antiobody was detected more frequently in the pationts with chronic liver diseases than in those with collagen diseases, In some serum samples, the antibody titres were higher in the presence of free Ca^<++> than in its absence, suggesting that they recognized conformations of CaM. Using CaM-conjugated agarose column, we found that there were three types of antibody : antibodies that bound to Ca^<++>-bound CaM, Ca^<++>-free CaM and both of these. CaM antiobody inhibited phosphodiesterase activity. However it remained for future study whether CaM antibody was involved in the liver cell injury or not.
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