| Project/Area Number |
61480189
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
OKAZAKI Yukinori First Department of Internal Medicine, Yamaguchi University School of Medicine, Associate Professor, 医学部, 助教授 (40035241)
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| Co-Investigator(Kenkyū-buntansha) |
嶋田 正勝 山口大学, 医学部附属病院, 医員
TADA Masahiro First Department of Internal Medicine, Yamaguchi University School of Medicine,, 医学部, 助手 (90179705)
SHIMADA Masakatsu First Department of Internal Medicine, Yamaguchi University School of Medicine,
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Photodynamic theraph(PDT) / Electron spin resonance(ESR) / Porphyrin polymer(N-HpD) / MBT-2 tumor / C3H / He mouse / Argon laser / Argon dye laser / ヘマトポルフィリン誘導体 / 電子スピン共鳴法 / 色素レーザー / 【R_2】.【NO_2】ラジカル / 実験小動物用内視鏡 / 電子スコープ |
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| Research Abstract |
We studied the mechanism of photodynamic therapy(PDT) for gastric cancer using electron spin resonance(ESR).
Characteristic spectrum of ESR was detected in the biopsy specimen after PDT of one case of gastric cancer. So, we investigated this ESR spectrum about experimental tumors which were subcutaneous MBT-2 tumors transplanted to C3H/He mice, because the clinical case of PDT was very limited by its indication.
Porphyrin polymer(N-HpD) of 5mg/Kg was injected into the abdominal cavity of mice accompanied with subcutaneous tumors, and then these tumors were irradiated each 12, 24, 36 and 48 hours later by argon laser and argon dye laser respectively After the irradiation, the intensity of ESR spectrum for these tumors examined.
As a result, the group after 12 hours revealed the highest intensity of ESR spectrum in comparison with control group. And, the next was the group after 24 hours. The intensities of ESR spectrum in the group after 36 hours and 48 hours were almost same to that of co
… More
ntrol group. This result means that N-HpD in the tumor cell brought about some chemical reactions by laser irradiation and produced the radical which was considered as R_2NO radical.
No difference about the effect between argon laser and argon dye laser were in this study.
And then, we tried to examine gastroscopy of rat because we had to confirm the gastric cancer in the stomach of experimental animal as the model of clinical case for ESR examination. At first, we used the petscope which was the smallest conventional scope with 4mm of caliber. But, we could not observe the stomach of rat by this scope. The petscope was too large for rats, so they died from suffocation during veryshort time on its insert. We tried again to observe the stomach of rat using the needle scope which was made from glassfiber covered by steal pipe and had 2mm of caliber. Almost area was observed by use of this scope.
From above studies, it is considered that the analysis of ESR spectrum makes clearer about the mechanism of PDT when ESR spectrum is measured more in detail. And it is considered, too, that the measurement of ESR spectrum in sequence of the observation of experimental cancer in rat stomach makes the suitable condition of PDT for the patient with gastric cancer. Less
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