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Change of atrial natriuretic polypeptide receptors in the hypertensive state and the effect of drugs on these receptors.

Research Project

Project/Area Number 61480209
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionOkayama University,

Principal Investigator

OTA Zensuke  Third Dept of Int Med, Okayama Uni Med Sch, Prof., 医学部・第三内科, 教授 (90032870)

Co-Investigator(Kenkyū-buntansha) MAKINO Hirofumi  Third Dept of Int Med, Okayama Uni Med Sch, Instructor, 医学部, 助手 (50165685)
HIRAKAWA Syuzo  Third Dept of Int Med, Okayama Uni Med Sch, Instructor, 医学部附属病院, 助手 (60156651)
HASHIMOTO Kozo  Third Dept of Int Med, Okayama Uni Med Sch, Lecturer, 医学部附属病院, 講師 (60033370)
OGAWA Norio  Inst for Neurobiology, Okayama Uni Med Sch, Associate Prof., 医学部, 助教授 (90033208)
OGURA Toshio  Third Dept of Int Med, Okayama Uni Med Sch, Instructor, 医学部, 助手 (80214097)
高取 克彦  岡山大学, 医学部附属病院, 医員
鈴木 信也  岡山大学, 医学部, 助教授 (70032901)
片山 江里子  岡山大学, 医学部附属病院, 医員
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1986: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsatrial natriuretic polypeptide / hypertension / kidney receptor / autoradioqraphy / alpha-adrenerqic receptor / spontaneously hypertensive rat / DOCA salt hypertension / 画像解析装置 / 高血圧自然発症ラット(SHR) / 受容体 / 降圧利尿剤 / オートラジオグラフィー(ARG) / 高血圧自然発症ラット(SHR)腎
Research Abstract

To elucidate the characteristics of renal receptors for atrial natriuretic polypeptide(ANP) and their role in the regulation of blood pressure, we performed radiolabeled receptor assay(RRA) on rat renal membrane fractions and in vitro autoradiography (ARG) on frozen sections using 125I-labeled ANP. The density of 125I-labeled ANP in the kidneys of spontaneously hypertensive rats(SHR) was lower in all age groups(3-4, 5-7 and 12-15 weeks) than of control rats, suggesting that SHR have fewer binding sites genetically. A decrease in the number of ANP binding sites in 12 week-old SHR was observed following treatment with the anti-hypertensive diuretic indapamide. In DOCA salt hypertensive rats, plasma concentration of ANP was increased remarkably and the density of ANP receptors in the kidney was decreased. These result suggest that in this model ANP receptor activity was down regulated with increasing blood pressure. We also detected the ANP binding sites in the glomerulus of renal biopsy specimens, although a relationship between patients morbidity and the degree of ANP in the kidney was not determined. Differences of alpha adrenergic receptors in the kidneys of hypertensive and prehypertensive SHR were investigated and compared with those for ANP receptors. Both alpha1 and alpha2 adrenoceptors were more numerous in pre-hypertensive SHR as determined by computerized analysis of in vitro macro ARG of [3H -bunazosin and [3H -yohimbine.
We concluded that ANP play an important role in the control and progression of the hypertensive state.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Takatori,K.,Ogura,T.,Katayama,E.,Ota,Z.,Mizukawa,K.,Ogawa,N.: "Autoradiographic localization and computerized quantification of specific binding sites for alpha-1 and alpha2-adrenoceptors in spontaneously hypertensive rat kidney." The Journal of International Medical Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Katayama,E.,Ogura.,T.,Ota,Z.: "Characteristics of rat kidney dopamine receptors and the effects of renal denervation and dopamine infusion on these receptors." Nephron. Vol.53. 358-363 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Katayama,E.,Ogura,T.,Takatori,K.,Mitsui,T.,Yamamoto,I.,Ota,Z.,Ogawa,N.,Mizukawa,K.: "Localization of α1-adrenoceptor in the rat kidney:in vitro autoradiography using [3H]-bunazosin." Research Communication in Chemical Pathology and Pharmacology. Vol.64. 205-213 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogura,T.,Yamamoto,I.,Ota,Z.: "Developmental change of kidney receptor for atrial natriuretic factor in spontaneously hypertensive rat." Hypertension. Vol.13. 449-455 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogura,T.,Katayama,E.,Mitsui,T.,Takatori,T.,Ota,Z.,Mizukawa,K.,Ogawa,N.: "Properties of [3H]bunazosin binding in rat kidney." Clinical Therapeutics. Vol.10. 559-567 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 太田 善介: "腎疾患における心房性ナトリウム利尿ペプチドに関する臨床的実験的研究" 昭和63年度両備てい園記念財団研究助成金による研究報告書. 35-43 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takatori,K., Ogura,T., Katayama,E., Ota,Z., Mizukawa,K. and Ogawa,N.: "Autoradiographic localization and computerized quantification of specific binding sites for alpha1- and alpha2-adrenoceptors in spontaneously hypertensive rat kidney." The Journal of International Medical Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Katayama,E., Ogura,T. and Ota,Z.: "Characteristics of rat kidney dopamine receptors and the effects of renal denervation and dopamine infusion on these receptors." Nephron. Vol.53. 358-363 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Katayama,E. Ogura,T., Takatori,K., Mitsui,T., Yamamoto,I., Ota,Z., Ogawa,N. and Mizukawa,K.: "Localization of 1-adrenoceptor in the rat kidney: in vitro autoradiographyu sing[3H -bunazosin." Research Communications in Chemical Pathology and Pharmacology. Vol.64. 205-213 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogura,T., Yamamoto,I. and Ogawa,N.: "Developmental change of kidney receptor for atrial natriuretic factor in spontaneously hypertensive rat." Hhpertension. Vol.13. 449-455 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ogura,T., Katayama,E., Mitsui,T., Takatori,K., Ota,Z., Mizukawa,K. and Ogawa,N.: "Properties of [3H bunazosin binding in rat kidney." Clinical Therapeutics. Vol.10. 559-567 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Ogura;E.Katayama;T.Mitui;K.Takatori;Z.Ota;K.Mizukawa;M.Ogawa: Clinical Tnerapeutics. 10. 559-567 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Ogura;I.Yamamoto;N.Ogawa: Hypertension. 13. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Mizukawa;T.Ogura;I.Yamamoto;T.Mitui;E.Katayama;Z.Ota;N.Ogawa: Reguratory Peptides. 21. 167-172 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] E.Katayama;T.Ogura;K.Takatori;T.Mitui;I.Yamamoto;Z.Ota;et al.: Res.Commun.Chemi.Pathol. Pharmacol.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] E.Katayama;T.Ogura;Z.Ota: Nephron.

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Takatori;T.Ogura;E.Katayama;Z.Ota;K.Mizukawa;M.Ogawa: Renal Pnysiology.

    • Related Report
      1988 Annual Research Report
  • [Publications] T.Ogura;T.Mitsui;I.Yamamoto;E.Katayama;Z.Ota;N.Ogawa: Life Sciences. 40. 233-238 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] I.Yamamoto;T.Ogura;Z.Ota: Res.Commun.Chemi.Pathol.Pharmacol.56. 291-298 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Barry M.Brenner;Jhon H.Laragh ED.;T.Ogura;T.Mitsui;I.Yamamoto;E.Katayama;Z.Ota;N.Ogawa: BIOLOGICA AVTIVE ATRIAL PEPTIDES. 493-495 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] K.Hashimoto;T.Hattori;M.Sugawara;S.Suemaru;T.Takao;J.Kageyama;Z.Ota: Regulatory Peptide. 17. 53-60 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] K.Mizukawa;T.Ogura;I.Yamamoto;T.Mitsui;E.Katayama;Z.Ota;N.Ogawa: Reguratory Peptide. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 小倉俊郎,三井健史,山本一郎,片山江里子,太田善介,小川紀雄: 日本臨床代謝学会記録. 23. 130-131 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Ogura;T.Mitsui;I.Yamamoto妙E.Katayama;Z.Ota,N.Ogawa: Res.Commun.Chemi.Pathol.Pharmacol.54. 291-298 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Ogura;T.Mitsui;I.Yamamoto;E.Katayama;Z.Ota;N.Ogawa: Life Sciences. 40. 233-238 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Hattori;K.Hashimoto;H.Inoue;M.Sugawara;S.Suemaru;J.Kageyama;Z.Ota: Endocrinol.Japn.33. 533-539 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Hashimoto;T.Hattori;M.Sugawara;S.Suemaru;T.Takao;J.Kageyama;Z.Ota: Regulatory Peptide. 17. 53-60 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Yamamoto;T.Ogura;Z.Ota: Res.Commun.Chemi.Pathol.Pharmacol. in press.(1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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