| Co-Investigator(Kenkyū-buntansha) |
ARITA Mikio Lecturer, Department of Cardiology, 医学部, 講師 (40168018)
KUCHII Masato Lecturer, Department of Cardiology, 医学部, 講師 (90073684)
MASUYAMA Yoshiaki Professor, Department of Cardiology, 医学部, 教授 (30089164)
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| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Research Abstract |
In order to investigate role of vascular smooth muscle cells(VSMCs) in the pathogenesis of hypertension and atherosclerosis, the synthesis of DNA, protein and qlycosaminoglycans(GAGs) were tested in cultured VSMCs from spontaneously hypertensive rats(SHR) and Wistar Kyoto Rats(WKY). 1.Cultured VSMCs from SHR showed increased synthesis in DNA, protein and GAGs compared with WKY. 2. Dultured VSMCs from SHR exposed to centrifugal force, as a simulation of blood pressure, synthesized more DNA, protein and GAGs than that from WKY. 3.Noradrenaline, added to VSMCs, inhibited DNA svnthesis, while pretreatment by <beta>-blockade testored it. Noradrenaline treatment showed no chages in protein synthesis, though pretreatment by <alpha>-blockade inhibited and <beta>-blockade promoted it. GAGs synthesis was increased by noradrenaline, while pretreatment by <beta>-blockade restored it, and that by <alpha>-blockade in SHR accerelated it. These effects of <beta>-stimulation was confirmed by the experiments using db-cAMP or theophylline. 4.Vasopressin or angiotensin II added to cultured VSMCs potentiated DNA synthesis in WKY, dose-dependently, while not in SHR. Insulin augmented DNA synthesis both in SHR and WKY. 5.SHR-conditioned medium potentiated DNA synthesis in WKY, dosedependently. 6.VSMCs from SHR showed higher levels of concentration of cytosolic free calsium than those from WKY. These data suggest that VSMCs from SHR have more potent synthetic activities in DNA, protein and GAGs, against not only physical but also chemical stimulations, and that VSMCs from SHR have the characteristcs that they easily move from G_0 to G_1.
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