| Project/Area Number |
61480222
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Tokai University |
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Principal Investigator |
KATO Shunichi Tokai University School of Medicine, Assistant Professor, 医学部・小児学教室, 講師 (70096212)
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| Co-Investigator(Kenkyū-buntansha) |
YABE Miharu Tokai University School of Medicene, Instructor, 医学部・小児科学教室, 助手 (40172514)
INOKO Hidetoshi Tokai University School of Medicine, Assistant Professor, 医学部移・植学教室II, 講師 (10101932)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | gene transfer / electroporesis / リポゾーム法 / レトロウイルスベクター法 / 高電圧パルス法 / 骨髄幹細胞 / 骨髄細胞 / HLA抗原 |
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| Research Abstract |
Bone marrow transplantation has benn applied for several genetic diseases as a curative therapy in recent years. Because of lack of suitable histocompatible donors, BMT cannot be applied to all potential patients. An alternative treatment modality in such patients involves transfer of cloned genes into the patients' hematopoietic stem cells, namely gene therapy.
We have developed electric field-mediated DNA transfer technique as a model system of gene therapy, and could demontrate the successful introduction and expression of the HLA class II genes into human and mouse bone marrow cells in in vitro culture system at a frequency of 7%. The injected mouse cells were infused back into the lethally irradiated mice and then were shown to express the HLA genes stably in peripheral blood cells although not as efficient as expected(0.02%). This electroporation technique offers several advantages;it is very simple and free from oncogenicity in contrast to retrovirus-mediated gene transfer and avoids some of the disadvantages associated with other methods of gene transfer. These results suggest that electric field-mediated DNA transfer is the most suitable technique for gene therapy.
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