| Project/Area Number |
61480249
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Shinshu University School of Medicine |
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Principal Investigator |
HASHIZUME Kiyoshi Associate Proffesor, Department of Geriatrics, Endocrinology and Metabolism, Shinshu University School of Medicine, 医学部附属病院老年科, 講師 (60092889)
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| Co-Investigator(Kenkyū-buntansha) |
山内 恵史 信州大学, 医学部老年科, 助手 (30191191)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Cytosolic 3,5,3'-triiodo-L-thyronine(T_3)-binding protein(CTBP) / Acceptor protein / nuclear T_3 receptor / NADP / NADPH / Counterregulation / counterregulation / 遣伝子発現調節 / 甲状腺ホルモン受容体 / 細胞質受容体 / 細胞核受容体 / 遺伝子発現 / 受容体受容蛋白 / ADPリボシレーション / 核基質 / ホルモン能態輸送 / 活性型および非活性型受容体 / DNA修復 / リボシレーション / 情報処理機構 |
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| Research Abstract |
Cytosolic 3,5,3'-triiodo-l-thyronine(T_3)-binding protein(CTBP) was purified from rat kidney. The molecular weight of CTBP was estimated by gel exclusion study, sucrose density gradient centrifugation and SDS-polyacrylamide gel electrophoresis, and gave a result that MR was 58,000,which was different from that of nuclear T_3 receptor. The purified CTBP was activated by NADPH or by NADP in the presence of -SH residue. The maximal binding capacity was calculated and gave a result that one molecule of CTBP can bind one molecule of T_3. The affinity of T_3 binding to CTBP was identical to that of nuclear T_3 receptor, although nuclear T_3 receptor had a different molecular size.
These pyridine nucleotides increased the maximal binding capacity without changes in the affinity constant. NADPH-activated CTBP did not bind to nuclei. However, the NADP-activated CTBP could bind to the nuclei. By extraction with 0.5 M NaCl, acceptor protein for NADP-activated CTBP was isolated. The molecular weight of the acceptor protein was 200,000. NADPH-activated CTBP, therefore, may play a role as a reservoir for intracellular T_3. And NADPH-activated CTBP may play a role in the regulation of gene expression induced by T_3 binding to its specific receptor in nuclei. NADP and NADPH take play as counterregulatory factors for T_3-induced gene expression.
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