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Study on a new release mechanism abnormality (unresponsiveness to thromboxane A_2) of platelet

Research Project

Project/Area Number 61480257
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionNiigata University

Principal Investigator

HATTORI Akira  First Department of Internal Medicine, 医学部, 助教授 (10018731)

Co-Investigator(Kenkyū-buntansha) KOIKE Tadashi  First Department of Internal Medicine, 医学部付属病院, 助手 (30170161)
TAKAHASHI Hoyu  First Department of Internal Medicine, 医学部付属病院, 助手 (70163285)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsPlatelet dysfunction / Ca^<++> mobilization / unresponsiveness to thromboxane A_2 / 放出機構障害 / 血小板 / 出血性素因 / 放出反応 / Ca2+動員 / Aequorin 08711794000600長山礼三: 第28回日本臨床血液学会総会抄録集. 281 (1986) / 【Ca^(++)】動員 / Aequorin
Research Abstract

In order to clarify the mechanism of defect in a new type of platelet release mechanism abnormality reported by us in 1980-1981, intracellular Ca^<++>,[Ca^<++>]i, mobilization was analysed in cases of thrombin, A23187, stable thromboxane analog SAT_2 or arachidonate-induction using aequorin loading method. In presence of L mM Ca^<++>, patient (P) platelets showed normal [Ca^<++>]i elevation (influx and intracellular mobilization) in response to thrombin, but suppressed one to low concentration of A23187 or STA_2. The decreased mobilization in absence of Ca^<++> was enhanced to normal by addition of 2mM Ca^<++> or by adrenalin potentiation. TMB-8 which caused reduced aggregation similar to P platelets in normal platelets was not sensitive to adrenalin potentiation. Various but slight degree of abnormalities in [Ca^<++>]i mobilization were found in Bernard-Soulier syndrome, cyclooxygenase deficiency, and Hermansky-Pudlak syndrome, suggestkng different characters of Ca^<++> mobilization measured by present method. In conclusion P platelets were partially defective in Ca^<++> mobilization (probably Ca influx) in responses to A23187 or STA_2, and different from TMB-8 treated normal paltelets. Closer relation between[Ca^<++>]i mobilization and aggregation or release in various agonists is neccessary to be investigated.

Report

(3 results)
  • 1988 Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 長山礼三 他: 第28回日本臨床血液学会総会抄録集. 281 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 服部晃 他: 第28回日本臨床血液学会総会抄録集. 282 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nagayama,R.,et α-.: Thrombosis and Haemostasis. 58. 478 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 服部晃 他: 血小板1987-血小板と脂質-. 166-188 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 服部晃 他: 病態生理. 6. 577-579 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hattori,A.et al.: "Post thrombin-binding delay in shape change and intracellular Ca^<++> mobilization in Bernard-Soulier syndrome." Proc. of 28th Meeting of Jap. Soc. Clin. Haematol.282 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hattori,A.et al.: "Platelet cyclo-oxygenase deficiency. Studies on the first case in Japan." Platelet 1987 -- Platelet and Lipids (Kagakuhyoronsya, Tokyo). 166-188 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hattori,A.et al.: "Studies on a new type of platelet release mechanism defect (irresponsibility to thromboxane A_2)." Medicina Philosophica. 6. 577-579 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nagayama,R.et al.: "Platelet ionized calcium mobilization (Aequorin method) in patients with primary platelet dysfunction" Thrombosis and Haemostasis. 58. 478 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 服部晃: 第28回日本臨床血液学会総会抄録集. 282 (1986)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nagayama,R.: Thrombosis and Haemostasis. 58. 478 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 服部晃: 日本血液学会雑誌. 50. 282 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 長山礼三: 第28回 日本臨床血液学会総会抄録集. 281 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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