| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Mineo Dept. of Medical Resources, Faculty of Pharmaceutical Sciences, Toyama Medical &, 薬学部, 助教授 (00019118)
YAMAMOTO Keiichi Dept. of Surgery, Toyama Medical & Pharmaceutical University Professor, 医学部(第1外科), 教授 (80019885)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1988: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Research Abstract |
INVESTIGATION OF TUMOR SPECIFIC SUBSTANCE IN GLYCOPROTEIN FRACTION OF SERA AND BRONCHIAL WASHING IN PATIENTS WITH DIFFERENT CARCINOMAS.
The sera of malignant, non-malignant and normal patients were analyzed, using high performance liquid chromatography (HPLC) with TSK-gel G 3000 SW column. This analysis demonstrated a fraction which shows a significantly low value at 34 minutes of retention time (RT) in patients with different carcinomas. An estimate of the molecular weight of this compound using several standard proteins revealed the substsnse has a molecular weight less than 20,000. RE-examination using a TSK-gel G 2000 SW column, which has an excellent analytical ability in the low molecular weight zone, made it possible to further divide the RT34 fraction into three components, occurring at RTs of 32 minutes, 34 minutes and 35 minutes. Among these three fractions, the 34 minutes RT fraction showed a significantly increased value in a patients with cancer. Those fractios at RT 32 min
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utes and RT 35 minutes showed a significantly low value. It was also found that the fraction at RT 34 minutes on a TSK-gel G 2000 SW column, revealed a high lavel in bronchial washings, which obtained from the affected side, malignant pleural effusion and ascites.
Consequently, we considered the possiblility that a cancer specific substance may be contained in the 34 minute RT fraction on a TSK-gel G 2000 W column. The molecular weight is less than 20,000, and it is entirely different substance from CEA (molecular weight 20,000), AFP (molecular weight 70,000), and other conventional cancer specific antigens that have been reported. In order to fractionate a large amount of 34 minutes RT material, we eliminate proteins with a molecular weight of more than 30,000 by filtration using a molecular cutter, and evaluate the filtrates, however we have not yet identified it. At present, we are now extracting this fraction in a gel filtration using a XK 26/100 column through sephacryl s-100 HR. After a large quantity of this substance is able to collect, we hope to identify the specific substance in sera of patients with different carcinomas. We hope these facts may lead to a clinically applicable study as a diagnostic aid for cancer, and further to apply it in cancer therapy in near future. Less
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