| Project/Area Number |
61480298
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
OGA Koichi Kyoto Prefectural University of Medicine, 医学部, 助教授 (40079843)
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| Co-Investigator(Kenkyū-buntansha) |
KANKI Yutaka Kyoto Prefectural University of Medicine, 医学部, 助手 (40169785)
WADA Yukio Kyoto Prefectural University of Medicine, 医学部, 助手 (90167213)
SHIRAKATA Shuji Kyoto Prefectural University of Medicine, 医学部, 講師 (80117887)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | Heart-lung transplantation / Japanese monkey / Histochemistry / モノクロナル抗体 / 心肺移植 / サル / 移植心の神経分布の変化 / モノクロナール抗体 / 酵素組織化学法 |
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| Research Abstract |
Orthotopic heart-lung transplantation is performed using Japanese monkeys. Bleeding was the first trouble, and weaning from extracorporeal circulation was difficult. By technical improvement including the proper selection of sutures, and using of another monkey as a blood donor, hemostasis was achieved, and weaning from ECC became easy. Then the next trouble was the deterioration of lung.
Atelectasis, edema, bleeding developed from just after recirculation, and resulted in the low PaO_2, elevation of inspiratory pressure of respirator. All cases died within 6 hours after weaning of ECC. Care was made not to touch the lungs as possible during resection and transplantation of heart and lungs, and Ep3 solution which was reported by Tohoku University was used to protect the lungs from injury caused by ischemia. Ice slush was also used to cool the lungs during transplantation. These procedures appearently improved the lungs condition of the heart and lungs after ECC, 44 hours was the longest survival time. Investigation of the early detection of rejection and the morphological aspects of nerve fibers in the transplanted, denervated heart and lungs were made by next experiments.
Left hemi-lung was transplanted orthotopically, and the heart was implanted in the abdomen. Five days after transplantation, nerve fibers in the transplanted bronchus and atrio-ventricular node of the heart showed almost normal Ch-E activity. Investigation to detect the rejection was made by histochemistry and using the monoclonal antibody.
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