| Project/Area Number |
61480300
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | Kinki University School of Medicine |
|---|
Principal Investigator |
SHIROTANI Hitoshi Kinki University school of Medicino , Professor, 医学部, 教授 (60113156)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Osamu Kinki University School of Medicine , Assistant Professor, 医学部, 病院講師 (90177402)
|
|---|
| Project Period (FY) |
1986 – 1987
|
| Project Status |
Completed (Fiscal Year 1987)
|
| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥4,200,000 (Direct Cost: ¥4,200,000)
|
|---|
| Keywords | nuclear magnetic resonance / reperfusion injury / myocardial edema / 保存心 / 再灌流障害 |
|---|
| Research Abstract |
The reperfusion injury is one of the very important problems for open heart surgerys and heart transplantations. The preservation of reperfusion injury will lead these operations to good results. Because of this we have done research on myocardial edema caused by reperfusion injury. We have used nuclear magnetic resonance spectorometer (NMR) to evaluate intramyocardial high energy phosphate in rabit hearts. At this time we attempted to use NMR to evaluate intramyocardial edema by making good use of shift reagents. Poly-L-aspartic acid and poly-L-glutamic acid were used to distinguish between intravascular space and intramyocardial space. The results of thw experiment with some shift reagents is as follows.
shift(ppm) broadning (Hz)
0.1MCe 1.0
Nd 2.2
Fe(II) 4.3 62
Fe(III) wide
Co 5.4 76
Ni 2.1 89
Cu 0.8 80
Mn wide
As a result, we decided to use 40mM Co which is combined with poly-L-glutamic acid.
|
