| Project/Area Number |
61480340
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Kyoto University |
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Principal Investigator |
YOSHIDA Osamu Faculty of Medicine, 医学部, 教授 (70025584)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIO Yasunori Faculty of Medicine, 医学部, 助手 (50180584)
MIYAKAWA Mieko Faculty of Medicine, 医学部, 講師 (10127144)
OKADA Kenichiro Faculty od Medicine, 医学部, 助教授 (60026838)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | Clonogenic assay / Chemical carcinogenesis / Tumor-promoter |
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| Research Abstract |
Colony formation in soft agar was observed with bladder cells prior to papilloma development in rats treated with N- Butyl-N-(4-hydroxybutyl)nitrosamine(BBN), and the number of colonies formed was dependent on the quantity of BBN consumed. It was then demonstrated that sodium saccharin and several amino acids, which are known to act as rat bladder cancer promoters, induce colony formation of rat bladder cells after treatment with subcarcinogenic dose of BBN, suggesting that there may be a correlation between tumor-promoting activity and colony inducing activity.
Effect of anti-tumor-promoter on colony formation was then assessed in two-stage mouse skin carcinogenesis experiment. Soft agar colony formation of mouse epidermal cells induced by 12-0- tetradecanoylphorbol-13-acetate(TPA) after single application of 7,12-dimethylbenz[a]anthracene(DMBA) was inhibited by treatment of skin with retinoic acid. Effect of dietary retinyl acetate. nordihydroguaiaretic acid(NDGA) and quinacrine hydrochloride on soft agar colony formation of rat bladder cells was also eaaluated. Soft agar colony formation of bladder cells induced by sodium saccharin after treatment with subcarcinogenic dose of BBN was inhibited by the administration of retinyl acetate or NDGA. suggesting the possible anti-tumor-promoting activity of the two agents.
Thus, an application of clonogenic assay to carcinogenesis experiment could be useful to analyse early events in carcinogenesis and provide a short-term screening method for detecting new tumor-promoters and anti-tumor-promoters.
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