| Project/Area Number |
61480348
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yamanashi Medical University |
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Principal Investigator |
JUNZO Kato Yamanashi Medical University, 医学部, 教授 (80082121)
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Noriaki Yamanashi Medical University (until 63.6), 医学部, 助手 (00161836)
MITSUHASHI Naoki Yamanashi Medical University (Present position, Tokyo University School of Medic, 医学部, 講師 (60114667)
YOSHIDA Koji Yamanashi Medical University, 医学部, 助手 (60166949)
KINOSHITA Toshihiko Yamanashi Medical University, 医学部, 講師 (90186289)
YASUMIZU Takehiko Yamanashi Medical University, 医学部, 助教授 (80107705)
小川 恵吾 山梨医科大学, 医学部, 助手
野沢 昭 山梨医科大学, 医学部, 助手
笠井 剛 山梨医科大学, 医学部, 助手 (20194699)
井川 名記 東京大学, 医学部, 助手 (80168096)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | Estrogen receptor / Progesterone receptor / Monoclonal antibody / Immunological Assay / human endometrium / Receptor messenger RNA / メッセンジャーRNA / ステロイドホルモン / 単クローン抗受容体抗体 / 受容体酵素免疫化学的測定法 / 免疫組織化学的ステロイド受容体検出法 / 子宮筋層 / 受容体局在 |
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| Research Abstract |
1. Enzyme immunological measurement of estrogen receptors in the human endometrium by the use of monoclonal antibody: - The concentrations of cytosol, nuclear, and total estrogen receptors (ER) determined by EIA assay were compared with those by a conventional ligand binding assay on LH20. There was good correlation between the EIA and LH20 assay data, indicating the usefulness of the ER-EIA because of no use of radioisotope and its simplicity.
2. Immunocytochemical detection of human endometrial estrogen receptors:- (1)Reactivity by anti-ER monoclonal antibody was observed only in nuclei of the gland epithelium and stroma cells. this finding supports the nuclear localization of estrogen receptors in the uterus. (2)Detection and intensity of ER staining correlated with the concentrations by the LH20 assay. This semi-quantitative ER assay is very useful, particularly when only a small amount of tissues is available. (3)Estrogen receptors in endometrial semars were positively stained. Clinical appli-cation is possible, e.g, for hormone responsiveness of the endometrium.
3. Progesterone receptor determination by enzyme immunoassay in human endometria: Correlation between the EIA and LH20 values was variable. Careful application of the method is needed for PR determination in human uterine tissues.
4. Estrogen receptors in pathologic uterine tissues and tamoxifen challenge test: - The ICA ataining was essentially similar to that in the normal tissue in terms of the receptor distribution and unclear localization. The tamoxifen challenge test revealed alternation in ER staining, indicating its usefulness for prediction of the efficacy of hormone therapy.
5. ER-mRNA in the uterus:- ER-mRNA tha been preliminary demonstrated in rat uterus.
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