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Ultrastructural study on the role of ground substance in the mineralization of collagenous matrix.

Research Project

Project/Area Number 61480376
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionTokyo Dental College

Principal Investigator

SHOSABURO Takuma  Tokyo Dental College, 歯学部, 教授 (60085694)

Co-Investigator(Kenkyū-buntansha) YASUO Miake  Tokyo Dental College, 歯学部, 講師 (00157421)
HISAKO Tohda  Tokyo Dental College, 歯学部, 講師 (80085828)
柳澤 孝彰  東京歯科大学, 歯学部, 助教授 (10096513)
Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsOsteodentin:Ground substance / Mineralization / Collagenous matrix / Non-collagenous matrix / Uitrastructure / ホスファターゼ / 超微構造 / 実験的骨様象牙質 / 球状石灰化物 / 類円形細胞 / 結晶含有小体 / 電子顕微鏡 / 分析電子顕微鏡
Research Abstract

The osteodentin, which is physiologically seen in the anterior extrimity of rat incisors and is formed experimentally by exposing the pulp chambers of rat molars, was used in the present study. The animals were fixed with glutaraldehyde; and their teeth were disseected out of the jaws, decalcified with EDTA, and prepared into ultrathin sections following usual procedures for electron microscopy. For demonstraton of phosphatase, the demineralized teeth were prepared into frozen sections of 20 m thick. The sections were incubated by means of the metal-salt technique for the demonstration of ACPase activity (Gomori, 1952) or ALPase activity (Mayahara et al., 1967), postfixed with OsO_4, and then ultrathin sections were preared for electron microscopy. Newly formed osteodentin was characterized by the presence of a number of globular clusters of apatite crystals distributed among mineralizing collagen fibrils. Most cells related to osteodentin formation had round profiles and a rich cytoplasm including a well-developed, ough-surfaced endoplasmic reticulum and a Golgi apparatus. Multivesicular bodies, cytosegresomes, and other sytoplasmic bodies classified by nagai (1970) as cytosome-3, -4 and -6, appeared in the cells. ACPase-activity reaction products were observed in such Golgi elements as lamellae, vacuoles, and vesicles. Cytosom-3, -4 and -6 and multivesicular bodies demonstrated reaction products. In the case of ALPase, reaction products were observed on the cell membraneand in cytosome-6.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] T. Yanagisawa: ELECTRON MICROSCOPY 1986. Vol. IV. 3067-3068 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Y. Miake: ELECTRON MICROSCOPY 1986. Vol. IV. 3075-3076 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T. Sawada: ELECTRON MICROSCOPY 1986. Vol. IV. 3077-3078 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 熊本順彦: 歯科学報. 86. 1823-1864 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] S. Takuma: J. Electron Microsc.36. 377-386 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 澤田 隆: 歯科学報. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 須賀昭一 編, 柳澤孝彰: "エナメル質, その形成, 構造, 組成と進化, 第2章エナメル質形成のメカニズム" クインテッセンス出版株式会社, 9 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 須賀昭一 編, 澤田隆: "エナメル質, その形成, 構造, 組成と進化, 第5章エナメル質の形成異常" クインテッセンス出版株式会社, 12 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takaaki Yanagisawa: "Intracellular apatite-containing vesicles related to osteodentin experimentally induced in rat molar pulp." ELECTRON MICROSCOPY 1986. 4. 3067-3068 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yasuo Miake: "Ultratructural changes in rat-incisor ameloblasts and odontoblasts following administration of vinblastine." ELECTRON MICROSCOPY 1986. 4. (1986 3075-3076)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takasi Sawada: "Ultrasdtructural changes in cementoblasts of rats to which antimicrotubular agents were administered." ELECTRON MICROSCOPY 1986. 4. 3077-3078 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yorihiko Kumamoto: "Electron-microscopic study of dentin-bridge formation observed during the self-healing process in exposed molar pulp." Shikwa Gakuho. 86. 1823-1864 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shosaburo Takuma: "Ultrastructural changes in smooth-ended and ruffle-ended maturing ameloblasts of rats after vinblastine administration." J. Electron Microsc.36. 377-386 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takasi Sawada: "Electron microscope study of changes of ameloblasts after administration of various kinds of drug." Shikwa Gakuho. in press. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takaaki Yanagisawa: Quintessence Publishing Co.Tooth enamel, its formation, sttructure, composition and evolution 2. Mechanisms of tooth enamel formation, 9 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takasi Sawada: Quintessence Publishing Co.Tooth enamel, its formation, sttructure, composition and evolution 5. Disturbed formation of tooth enamel, 12 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.Yanagisawa;Y.Kumamoto;T.Itaya;S.Takuma: Electron Microscopy 1986 T.Imura,S.Maruse,and T.Suzuki,eds.The Japanese Society of Electron Microscopy,Tokyo. vol.IV. 3067-3068 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 柳澤孝彰,熊本順彦,田中教順,東田久子: 歯科学報. 86. 1914 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Yanagisawa;T.Sawada;Y.Miake;S.Araki;S.Yama: J.Dent.Res.(1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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