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Alterations of non-collagenous phosphoproteins in human dentin coupled with the occurance of dentin hypersensitivity

Research Project

Project/Area Number 61480398
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionTokushima University

Principal Investigator

YOSHIYAMA Masahiro (1988)  Research Assistant, Dept. of Operative Dentistry, Tokushima Univ. School of Dentistry, 歯学部, 助手 (10201071)

内田 昭次 (1986-1987)  徳島大学, 歯学部, 教授 (90112060)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Reiko  Research Assistant, Dept. of Operative Dentistry, Tokushima Univ. School of Dent, 歯学部附属病院, 助手 (20200850)
ISHIKAWA Yasuko  Lecturer, Dept. of Pharmacology,Tokushima Univ. School of Dentistry, 歯学部, 講師 (40144985)
ISHIDA Hajime  Professor, Dept. of Pharmacology, Tokushima Univ. School of Dentistry, 歯学部, 教授 (70028364)
宇野 義治  徳島大学, 歯学部, 助手 (60183208)
井上 尚也  徳島大学, 歯学部, 助手 (00183187)
高橋 良明  徳島大学, 歯学部附属病院, 助手 (20175433)
長谷川 清  徳島大学, 歯学部附属病院, 助手 (20172885)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥5,700,000 (Direct Cost: ¥5,700,000)
KeywordsDentin / Dentin Hypersensitivity / Non-collagenous protein / Ca-binding ability / Osteonectin / Phosphophoryn / 管状膜様構造物
Research Abstract

In this investigation, we have studied the ultrastructural changes of dentinal tubules and tubular membranes in hypersensitive area in exposed human dentin, the purification of noncollagenous proteins from tubular membranes, and their characteristics.
The results obtained from the biopsy of hypersensitive teeth showed the opening of dentinal tubules and the appearance of tubular membranes in the hypersensitive area of exposed dentin.
On the other hand, dentinal tubules were occluded with superfine or rhombohedral crystals in naturally desensitized area on the same dentin surface and the mineralization of the dentinal tubules was obseved in the area.
These results suggested that the appearance of tubular membranes may be closely involved in occurance of dentin hypersensitivity through the control of remineralization of dentinal tubules.
Extraction and separation of noncollagenous proteins from tubular mem-branes were carried out to investigate the physiological function of tubular membranes. An osteonectin was purified from the noncollagenous proteins. We also purified osteonectin from human dentin powder. It was shown that dentin osteonectin was highly phosphorylated sialoprotein more than other hard con-nective tissues and the molecular weight of it was 23K daltons. Moreover, dentin osteonectin was able to bind calcium ions strongly and selectively.
In conclusion, the presence of dentin osteonectin in tubular membranes suggested that tubular membranes were closely related to the occurance of dentin hypersensitivity, and that dentin osteonectin may play important roles on the re-calcification of the dentinal tubules through the transportation of calcium ions.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 吉山昌宏,内田昭次,石田甫: 日本歯科保存学雑誌. 29. 567-580 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 吉山昌宏,長谷川清,高橋良明,正田二郎,井上尚也,宇野義治,石田甫,内田昭次: 日本歯科保存学雑誌. 29. 1218-1229 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 吉山昌宏,正田二郎,内田昭次,石川康子,石田甫: 日本歯科保存学雑誌. 30. 725-736 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.,Yoshiyama;J.,Masada;A.,Uchida;H.,Ishida: Journal of Dental Research(投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 内田昭次: "デンタルダイヤモンド増刊号:歯科における痛み 第1章 歯と歯周組織の痛み【○!2】知覚過敏のとき" デンタルダイヤモンド社, 26-29 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 正田二郎,吉山昌宏,内田昭次: "デンタルアスペクト 第3巻2号:特集 知覚過敏症への対応 1.知覚過敏症の診断とその処置法" デンタルアスペクト社, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Yoshiyama; A.Uchida; H.Ishida: "Tubular Membranes and Calcium-binding Phosphoproteins in Human Dentin" Japanese Journal of Conservative Dentistry. 29(2). 567-580 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Yoshiyama; K.Hasegawa; Y.Takahashi; J.Masada; N.Inoue; Y.Uno; H.Ishida; A.Uchida: "Ultrastructural Changes of Dentinal Tubules in Dentin Hypersensitivity" Japanese Journal of Conservative Dentistry. 29(4). 1218-1229 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Yoshiyama; J.Masada; A.Uchida; Y.Ishikawa; H.Ishida: "Purification and Characteristics of Osteonectin from Human Dentin" Japanese Journal of Conservative Dentistry. 30(2). 725-736 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Yoshiyama; J.Masada; A.Uchida; H.Ishida: "Ultrastructural Changes in Dentinal Tubules in Dentin Hypersensitivity I. SEM and Microradiographic Considerations" Journal of Dental Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] J.Masada; M.Yoshiyama; A.Uchida; H.Ishida: "Purification and Characteristics of Phosphophoryn from Human Dentin"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Uchida: Pain in Dentistry: Chapter I. Pain of Tooth and Periodontal tissue 2 Hypersensitivity. Dental Diamond Corporation, 26-29 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 正田二郎,吉山昌宏,内田昭次,石川康子,石田甫: 準備中.

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Yoshiyama;J.Masada;A.Uchida;H.Ishida: Journal of Dental Research.

    • Related Report
      1988 Annual Research Report
  • [Publications] 内田昭次: "デンタルダイヤモンド増刊号:歯科における痛み 第1章歯と歯周組織の痛み【○!2】知覚過敏のとき" デンタルダイヤモンド社, 26-29 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 正田二郎,吉山昌宏,内田昭次: "デンタルアスペクト 第3巻2号:特集 知覚過敏症への対応 1.知覚過敏症の診断とその処置法" デンタルアスペクト社, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 吉山昌宏他: 日本歯科保存学雑誌. 30. 725-736 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 吉山昌宏,内田昭次,石田甫: 日本歯科保存学会誌. 29. 567-580 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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