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Suppression of chromosome aberrations in chromosome aberration syndromes by microcell mediated chromosome transfer

Research Project

Project/Area Number 61480437
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Human genetics
Research InstitutionHokkaido University

Principal Investigator

YOSHIDA Michihiro C.  Faculty of Science, Hokkaido University, 理学部, 助教授 (60001765)

Co-Investigator(Kenkyū-buntansha) ABE Syuichi  Faculty of Science, Hokkaido University, 理学部, 助手 (80125278)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsChromosome aberrations / microcell / mapping / SCE / マッピング / マイクロセル / 染色体異常症候群 / DINAトランスフェクション / マツピング / マイトマイシンC / ミニセル / DNAトランスフェクション
Research Abstract

(1) Effect of the introduction of microcells into BS and FA cells: Microcells contained several numbers of chromosomes prepared from normal human diploid fibroblasts were fused to fibroblasts derived from Bloom syndrome (BS) or Faconi anemia (FA). Such fusions showed a dramatic reduction of each frequency of sister chromatid exchanges (SCEs) in BS-heterokaryons and of mitomcyin C (MMC)-induced chromosome aberrations (CAs) in FA-heterokaryons in comparison to that of parental BS of FA cells. The low levels of SCEs or MMC-induced CAs appeared in 2-day cultures after fusion and lasted fro several days, although a bimodal(high and low)level of SCEs in BS and MMC-induced CAs in FA heterokaryons was observed. The low level of SCEs or CAs in heterokaryons could be ascirbed to minicells that contained chromosomes reponsible for correction or suppression of BS- or FA-specific chromosome aberrations, while the transfer of minicells contained other chromosomes had no effect on suppression of the high levels of BS-specific SCEs or FA-specific CAs. Although it was not easy to identify chromosomes contributed to the normalization because of high frequency of pulverization of the transferred chromosomes, nos. 21 and 22 and sex chromosomes did not carry information of the normalization of BS-SCEs or FA-CAs. We are now trying to transfer minicells containing a single human chromosome prepared from man-mouse hybrid cells, in which human chromosomes had neo-marker.
(2) Superoxide dismutase (SOD) activity in BS and FA cells: To elucidate the genotoxic action of active oxygen species (superoxide radicals, O^-_), activity of SOD was measured in BS and FA cells. An abnormally elevated SOD activity was found in bothe BS and FA cells, suggesting that an excess of O^-_ due to perturbation of oxygen metabolism in those cells may lead to the elevation of SOD activity, albeit insufficient to eliminate the high O^-_ levels, which results in enhanced chromosome damage.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Matsukage,A.: Jpn.J.Cancer Res(Gann). 77. 330-333 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Abe,S.: Mutation Res.173. 37-47 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sekiguchi,T.: Expt.Cell Res.169. 395-407 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kaneda,Y.: Chromosoma. 95. 8-12 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Matsuoka,R.: Amer.J.Med.Genet.29. 369-376 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshida,M.C.: Proc.Natl.Acad.Sci.USA. 85. 4861-4864 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshida,M.C.: "Genetics of Human Tumors in Japan" Japan Scientific Socities Press, 225 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 吉田廸弘 口野嘉幸、手井久、櫻林陏之介: "実験操作ブロッティング法" ソフトサイエンス社, 361 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshida,M.C.: "Human HST1 (HSTF1) gene maps to chromosome band 11q13 and coamplifies with the INT2 gene in human cancer" Proc. Natl. Acad. Sci. USA. 85. 4861-4864 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yoshida,M.C.: "Chromosome localization of newly isolated protooncogenes in src family" Gann Monograph on Cancer Res.35. 209-220 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Matsuoka,R.: "Molecular cloning and chromosomal localization of a gene coding for human cardiac myosin heavy-chain" Amer. J. Med. Genet.29. 369-376 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Maru,Y.: "Evolution, expression, and chromosomal location of a novel receptor tyrosine kinase gene, eph" Mol. Cell Biol.8. 3770-3776 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tosu,M.: "Clonal isolation and characterization of myoblast-like reconstituted cells formed by fusion of karyoplasts from mouse tetratocarcinoma cells with rat myoblast cytoplasts" Cell Struct. Funct.13. 246-266 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yodhida,M.C.: Proc.Natl Sci USA. 85. 4861-4864 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tosu,M.: Cell Structure Funct.13. 249-266 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Maru,Y.: Mol.Cell.Biol.8. 3770-3776 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshida,M.C.: Gann Monograph Cancer Res.35. 209-220 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Abe,S.: Cancer Genet.Cytogenet.1988.

    • Related Report
      1988 Annual Research Report
  • [Publications] Abe,S.: Cancer Genet.Cytogenet.1988.

    • Related Report
      1988 Annual Research Report
  • [Publications] Sekiguchi,T.: Extl.Cell Res.169. 395-407 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Tani,K.: Biochem.Biophys.Res.Comm.143. 431-438 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kukita,A.: Hum.Genet.76. 20-26 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kaneda,Y.: Chromosome. 95. 8-12 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Ino,T.: Proc.Natl.Acad.Sci.USA. 84. 4264-4267 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yoshida,M.C.: J.Hered.78. 361-365 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 吉田〓弘;宮崎保;監修: "白血病細胞の生物医学的特性とその制御" 癌と化学療法社, 453 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 吉田廸弘;村松正実;編: "医科分子生物学" 南江堂, 390 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nishizawa,M.: Molec.Cell.Biol.6. 511-517 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Fukushige,C.: Molec.Cell.Biol.6. 955-968 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Semba,K.: Proc.Natl.Acad.Sci.USA. 83. 5459-5463 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yoshida,M.C.: Jpn.J.Cancer Res.77. 1059-1061 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Abe,S.: Mutation Res.173. 55-60 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 吉田迪弘: 蛋白質核酸酵素. 31. 1226-1244 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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