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Roles of leukotrienes in pathological states and therapentic application of 5-lipoxygenase inhibitors.

Research Project

Project/Area Number 61480442
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用薬理学・医療系薬学
Research InstitutionKitasato University School of Medicine

Principal Investigator

KATORI Makoto  Kitasato University School of Medicine Professor (Dept. Pharmacology), 医学部, 教授 (50050365)

Co-Investigator(Kenkyū-buntansha) NISHIYAMA Kazuo  Kitasato University School of Medicine Assistant professor (Dept. Pharmacology), 医学部, 助手 (20180656)
KAWAMURA Michiko  Kitasato University School of Medicine Assistant professor (Dept. Pharmacology), 医学部, 助手 (00154104)
ODA Takaharu  Kitasato University School of Medicine Assistant professor (Dept. Pharmacology), 医学部, 助手 (60185600)
MAJIMA Masataka  Kitasato University School of Medicine Assistant professor (Dept. Pharmacology), 医学部, 助手 (70181641)
UENO Akinori  Kitasato University School of Medicine Senior lecturer (Dept. Pharmacology), 医学部, 講師 (00112657)
原田 芳照  北里大学, 医学部, 助教授 (20050677)
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsLTC_4 / LTB_4 / Simple purification method for LT / Rat cardiac infarction / Ethanol-induced gastric lesion / Microcirculation of hamster cheek pouch / 5ーリポキシゲナーゼ阻害薬 / ラット遅延型アレルギー / 胸膜炎 / LTB_4 / 補体 / 白血球血管外遊走 / ロイコトリエンB_4 / 5ーリポキシゲナーゼ / ラット遅延型アレルギー性胸膜炎 / プロスタグランジン / 血漿キニン / 微小循環 / 多核白血球血管外游走 / ロイコトリエン測定法 / 5-リポキシゲナーゼ阻害薬 / ロイコトリエン(LT)【B_4】 / 心筋梗塞
Research Abstract

Roles of leukotrienes(LTs) in pathological states were analyzed in the following models: 1) Development of a simple purification method for RIA: Samples were acidified to PH 3.5 and LTs were separated by SEP-PAKC18. Recoveries of each LTs were more than 90%. 2) Tuberculin-induced rat pleurisy: Rats, sensitized by M.Tuberculosis H37 RA, were challenged by intrapleural injection of the same antigen. Plasma leakage showed three peaks at 3, 9-18, 48 hr and bradykinin and prostaglandins were involved, but LTs were not. Neither LTB4 nor complement was involved in neutrophils and mononuclear leukocyte migration in the exudate. 3) Rat cardiac infarction: ligation of left main coronary artery caused ischemia of 50% LV(left ventricle), which was reduced to 27% at 48 hr by reflow from surrounding tissue. Forty-two% of the LV became necrotic at 12 hr. Neutrophils were accumulated biphasically (up to 12 hr and 12-24 hr) in the cardiac tissue. LTB4, not LTC4, was generated in the cardiac tissue at a … More peak of 8 hr. A 5-lipoxygenase inhibitor (AA-861) attenuated the initial increase of neutrophils, whereas the second large increase was attributed to the complement component (C5a). The first increase of neutrophils played a pivotal role in enlargement of infarct size at 48 hr. 4) Ethanol-induced gastric lesion: Thirty of ethanol was placed in rat gastric lumen and this caused immediate cessation and congestion of blood in capillaries connected in the collecting venules. The LTC4 content in the stomach increased up to 4.4 ng/stomach (6 hold). LTC4 may constrict the collecting venules at the level of lamina muscularis. AA-861 attenuated the congestion of the stomach. 5) Microcirculation in hamster cheek pouch: Topical application of LTB4 over microvasculature caused rolling and adhesion o f neutrophils on the venular wall. More than 90% of the neutrophil adhered crossed the endothelial gap and stayed between endothelial cells and pericytes. They began to migrate to the interstitial space 30 min later. Less

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (60 results)

All Other

All Publications (60 results)

  • [Publications] K.Tanaka;A.Ueno;M.Katori: Prostaglandins. 31. 1099-1116 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Ueno;K.Tanaka;M.Katori: Prostaglandins. 31. 833-850 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 佐々木幸二、鹿取信、上野晃憲、重広世紀子、木川田隆一: 炎症. 6. 267-272 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Nagai;M.Katori: Adv.PG.TX.Res.17B. 905-907 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Oda;K.Nagai;M.Katori: In Microcirculation-an update. 1. 727-728 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Sasaki;A.Ueno;M.Katori;R.Kikawada: Cardi Ovasc.Res.22. 142-148 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Nagai;M.Katori: Int.J.Microcirc:Clin Exp. 7. 305-314 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Katori;T.Kanayama;K.Sasaki;A.Ueno;M.Takagi;S.Yamashina: Jarmacol.50. 234-238 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Oda;M.Katori: Microcirculation・annual 1988. 103-108 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 上野晃憲、佐々木幸二、鹿取信: 北里医学. 19. 327-335 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Katori;Y.Harada;A.Ueno;M.Majima;T.Oda;M.Kawamura: Dermatologica(suppl). 179. 60-63 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Katori;K.Nishiyama;A.Ueno;Y.Suzuki: J.Clin.Gastroenterol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Katori;T.Oda;K.Nagai: Agents and Actions. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 鹿取信、山本尚三 編: "プロスタグランジン研究法(上) プロスタグランジン研究法(下)" 東京化学同人現代化学, 253320 (19861987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 上野晃憲 鹿取信、山本尚三 編: "プロスタグランジン研究法(上)、III部、同定、定量法各論17.3、ロイコトリエンの分画と定量" 東京化学同人現代化学, 2 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 原田芳照 鹿取信、山本尚三 編: "プロスタグランジン研究法(上)、III部、同定、定量法各論20.1、連続表面灌流法によるPGの定量" 東京化学同人現代化学, 4 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 鹿取信: "炎症とプロスタグランジンー炎症の"黒子"をあやつる。…" スタンダードマッキンタイヤ, 99 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 日本語刊行委員会: "京都プロスタグランジン会議記録 プロスタグランジン、トロンボキサン、ロイコトリエン研究の進歩" 現代医療社, 599 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 鹿取信: "プロスタグランジンと血栓形成、Thrombiosis血栓症/背景" 治療, 6 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 鹿取信、山本尚三、佐藤和雄、阿部圭志: "プロスタグランジンの最近の研究の進歩" 講談社サイエンティフィク, 443 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Katori;Y.Suzuki;A.Ueno;K.Nishiyama: "Prostaglndinds and their role in the stomach.In Gastrointestinal Function.vol.6." Excerpta Medica, 12 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Tanaka; A. Ueno; M. Katori: "Roles of leukotrienes on two rat allergic inflammatory models; IgE-mediated and IgG-antigen complex-induced pleurisies." Prostaglandins. 31. 1099-1116 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Ueno; K. Tanaka; M. Katori: "Difference in the in vitro metabolism of leukotrienes in the exudates from allergic and nonallergic rat pleurisies." Prostaglandins. 31. 833-850 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Sasaki; M. Katori; A. Ueno; S. Shigehiro; R. Kikawada: "A Role of leukotriene B_4 in exacerbration of myocardial infarction of the rat." Jpn. J. Inflammation. 6. 267-272 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Nagai; M. Katori: "Chemotactic effect of leukotriene B_4 in the microcirculation." Adv. PG. TX. Res.17B. 905-907 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Oda; K. Nagai; M. Katori: "Time course of migration of polymorphonuclear leukocytes through venular endo-thelium in hamster cheek pouch." In Microcirculation - an update. 1. 727-728 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Sasaki; A. Ueno; M. Katori; R. Kikawada: "Detection of leukotriene B_4 in cardiac tissue and its role in infarct extention through leukocyte migration." Cardiovasc. Res.22. 142-148 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K. Nagai; M. Katori: "Possible chamges in the leukocyte membrane as a mechanism of leukocyte adhesion to the venular walls induced by leukotriene B_4 and fMLP in the microvasculature of the hamster cheek pouch." Int. J. Microcirc: Clin Exp. 7. 305-314 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Katori; T. Kanayama; K. Sasaki; A. Ueno; M. Takagi; S. Yamashina: "Biphasic accumulation of leukocytes in rat cardiac infarct tissue caused by leukotriene B_4 and complement." Jpn. J. Pharmacol.50. 234-238 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Oda; M. Katori: "Suppression of leukocyte adhesion to the venular walls by a cyclic AMP phosphodiesterase inhibitor in hamster cheek pouch." Microcirculation annual 1988. 103-108 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A. Ueno; K. Sasaki; M. Katori: "A simple method of eliminating substances of interference in the radioimmunoassay for leukotrienes as applied to biological specimens." Kitasato Med.19. 327-335 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Katori, Y. Harada, A. Ueno, M. Majima, T. Oda and M. Kawamura: "Modulation of plasma exudation by PGE_2 and that of leukocyte migration by LTB_4 in inflammatory models." Dermatologica (suppl). 179. 60-63 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Katori, K. Nishiyama, A. Ueno and Y. Suzuki: "Possible role of endogenous prostaglandins against ethanol injury in rat stomach." J. Clin. Gastroenterol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Katori, T. Oda and K. Nagai: "A site of action of dexamethasone on Jeukocyte extravasation in microcirculation." Agemts and Actions. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Katori; S. Yamamoto; K. Sato; K. Abe: Recent advances in prostaglandin research.Kodansha Scientific Publisher Co., 443 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Nagai,;M.Katori,: J,Microcirc.Clin.Exp.7. 305-314 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Sasaki,;A.Ueno,;M.Katori,;R.Kikawada,: Cardiovasc.Res.22. 142-148 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Hori,;H.Jyoama,;K.Yamada,;M.Takagi,;K.Hirose,;M.Katori,: Eur.J.Pharmacol.152. 235-245 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Katori,;T.Kanayama,;K.Sasaki,;A.Ueno,;M.Takagi,;S.Yamashina,: Jpn.J.Phrmacol.

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Katori,;Y.Harada,;A.Ueno,;M.Takahara,;T.Oda,;M.Kawamura,: Dermatologica.

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Katori,;M.Majima,;Y.Harada,;A.Ueno,: "A significant role of plasma Kallikrein-Kinin system in plasma exudation of rat carrageenin-induced pleurisy.Kinins V.Exp.Med.Biol." Plenum Press,

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Majima,;Y.Tani,;M.Katori,: "Degradation of hageman factor by chymotrypsin-like protease released from rat mast cells during degranulation.Kinins V. EXP.Med.Biol." Plenum Press,

    • Related Report
      1988 Annual Research Report
  • [Publications] M.Katori,;A.Hang,;Y.Harada,;M.Kawamura,: "Rapid increase in the intracellular calcium concentration by endogenous thromboxane A_2 in aggregation induced by threshold doses of thrombin." Trans membrane Signaling,

    • Related Report
      1988 Annual Research Report
  • [Publications] Majima.M;Tani.Y;Katori.M: Thrombosis Res. 46. 855-867 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sasaki.K;Ueno.A;Katori.M;Kikawada.R: Gardiovasc.Res.

    • Related Report
      1987 Annual Research Report
  • [Publications] Nagai.K;Katori.M: Microcirculation-clinical and experimental.

    • Related Report
      1987 Annual Research Report
  • [Publications] Hori.Y;Jyoyama.H;Yamada.K;Kageyama.H;Kurosawa.A;Hirose.K;Katori.M: Europ J.Phrmacol.

    • Related Report
      1987 Annual Research Report
  • [Publications] Oda.T;Nagai.K;Katori.K: "Microdirculation-an update(Time course of migration of polymorphonuclear leukocytes through venular endothelium in hamster chiik pouch)" Elsvier Science Pub, 727 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Katori.M;Nagai.K;Oda.T: "Microdirculation-an update(Roles of endothelial cells against platelets or polymorphonuclear leukocytes in microcirculation)" Elsvier Science Pub, 751 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kunio Tanaka: Prostaglandins. 31. 1099-1116 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Y.Uchida: Pharmacological Research Communications,. 18. 831-846 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yutaka Suzuki: Prostaglandins. 32. 401-414 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Kawana: Int.J.Tiss.Reac.8. 119-128 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Yozo Hori: Eur.J.Pharmacol.132. 47-52 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Akinori Ueno: Prostaglandins. 31. 833-850 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 川村道子: 血液と脈管. 17. 479-481 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 佐々木幸二: 炎症. 6. 267-272 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 谷義彦: 炎症. 7. 41-46 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 上野晃憲: Jap.J.Pharmacal.40. 88-

    • Related Report
      1986 Annual Research Report
  • [Publications] 山本尚三,鹿取信 編: "プロスタグランジン研究法(上)" 東京化学同人, 253 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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