| Project/Area Number |
61480457
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | University of Shizuoka (1987-1988)
Shizuoka Women's University (1986) |
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Principal Investigator |
ISEMURA Mamoru School of Food and Nutritional Sciences, 食品栄養科学部, 教授 (40028197)
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| Co-Investigator(Kenkyū-buntansha) |
KOSAKAI Masashi Tohoku University School of Medicine, 医学部, 助手 (20004624)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | Placenta / Fibronectin / Tissue organization / Aminotic epithelial cell / Laminin / Proteoglycan / 胎盤 / フィブロネクチン / コラーゲン / 基底膜 / フィブロヌクチン / 細胞外マトリックス成分 / 細胞増殖 |
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| Research Abstract |
In order to reveal the difference between plasma fibroenctin and tissue fibronectin, we isolated placental fibronectin and investigated the chemical sturctures of its carbohydrate chains. The oligosaccharides liberated by hydrazinolysis were radiolabeled and examined by sequential enzymatic degradation and methylation analysis. The results indicated that the placenatal and plasma fibronectins have quite different carbohydrate chains. The fibronectin synthesized in the tissue culture of the placenta also had the different sugar chains from those of plasma fibronectin as revealed by their binding nature to lectins. The immunohistochemical localization of fibronectin in the placenta identified fibronectin-producing cells including endothelial and fibroblastic cells. These results indicate that placenta tissue fibronectin is not derived from plasma, but that own tissue fibronectin is produced and retained. We have isolated and characterized a fibronectin-binding proteoglycan of the human placenta. to examine the biological activity of this proteoglycan, FL amnion epithelial cells were culauted on plastic dishes coated with fibronection or laminin with or without preincubation with this proteoglycan. FL cells were well attached to the dishes coated with fibronectin or laminin, but spread to a minor extent. When the coated dishes were preincubated with this proteoglycan, FL cells became spread well. Thus, the biological activities of the proteoglycan appeared to include the promotion of the cell spreading. Through our investigation on this project for 3 years, we have demonstrated that the placental tissue synthesizes and retains the fibronectin of its own, that the fibronectin has an important role in the placental tissue organizttion particularly in the epithelial formation, and that the function of the fibronectin is modulated by various extracellular amatrix components including a fibronectinbinding proteoglycan newly found by us.
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